Hanna Instruments HI 83742 Instruction Manual Download Page 7

7

A microprocessor controlled special tungsten lamp emits radiation which is first optically conditioned
and beamed to the sample contained in the cuvet. The optical path is fixed by the diameter of the
cuvet. Then the light is spectrally filtered to a narrow spectral bandwidth, to obtain a light beam of
intensity 

I

o

 or 

I.

The photoelectric cell collects the radiation 

I

 that is not absorbed by the sample and converts it

into an electric current, producing a potential in the mV range.
The microprocessor uses this potential to convert the incoming value into the desired measuring
unit and to display it on the LCD.
The measurement process is carried out in two phases: first the meter is zeroed and then the actual
measurement is performed.
The cuvet has a very important role because it is an optical element and thus requires particular
attention. It is important that both the measurement and the calibration (zeroing) cuvets are
optically identical to provide the same measurement conditions. Whenever possible use the same
cuvet for both. It is necessary that the surface of the cuvet is clean and not scratched. This to avoid
measurement interference due to unwanted reflection and absorption of light. It is recommended
not to touch the cuvet walls with hands.
Furthermore, in order to maintain the same conditions during the zeroing and the measuring
phases, it is necessary to close the cuvet to prevent any contamination.

degree Celsius
degree Fahrenheit
grams per liter. g/L is equivalent to ppt (part per thousand)
milliliter
Liquid Crystal Display

°C

:

°F

:

g/L

:

mL

:

LCD

:

ABBREVIATIONS

22

MEASUREMENT

MEASUREMENT

MEASUREMENT

MEASUREMENT

MEASUREMENT

• Press “RANGE” to select the parameter code “P4” for

total phenols, white wine (see page 13).

• After a few seconds the display will show “-0.0-”.

The meter is now zeroed and ready for measurement.

• Remove the cuvet from the instrument.

• Insert the sample (cuvet #2) into the holder and

close the lid.

#2

(Sample)

• Press READ/TIMER and “----” will blink during

measurement.

• The instrument directly displays concentration in g/L

of GAE (gallic acid equivalent) on the Liquid Crystal
Display.

Note

: If the phenols concentration is 0,750 g/L or

higher, please repeat the test following the instruction
for red wine (page 23).

• Fill an empty cuvet with deionized water and replace

the cap. This is the zero

zero

zero

zero

zero.

#1

(Zero)

• Insert the zero cuvet into the holder and close the lid.

• Press ZERO and “----” will blink on the display.

Summary of Contents for HI 83742

Page 1: ...Instruction Manual HI 83742 COLOR PHENOLS ISM for wine analysis w w w h a n n a i n s t c o m This Instrument is in Compliance with the CE Directives MAN83742R3 04 06 w w w h a n n a i n s t c o m...

Page 2: ...tampering or lack of prescribed maintenance are not covered If service is required contact your dealer If under warranty report the model number date of purchase serial number and the nature of the f...

Page 3: ...hazardous area The blinking will appear when the batteries power gets low When batteries are completely discharged 0 bAtt will appear and after two seconds the instrument is switched off Remove the b...

Page 4: ...ecommends usingthespecialwinesolventtominimizepossibleerrorsduetodilution Phenoldeterminationofwine Phenoliccompoundsareimportantforseveralreasonssincethey i affectthecolorofthewine ii have anastringe...

Page 5: ...ungstenicacid H3PW12O40 andphosphomolybdenicacid H3PMo12O40 to thequinoidform R O TheconcomitantreductionoftheFolin Ciocalteureagentcausesabluecolorin thesamplethatisproportionaltothetotalphenoliccont...

Page 6: ...s colorimeters is based on special subminiature tungsten lamps and narrow band interference filters to guarantee both high performance and reliable results Block diagram optical layout 23 TOTAL PHENOL...

Page 7: ...This to avoid measurement interference due to unwanted reflection and absorption of light It is recommended not to touch the cuvet walls with hands Furthermore in order to maintain the same conditions...

Page 8: ...to 20V DC 2 5 Watt FUNCTIONAL DESCRIPTION FRONT REAR INSTRUMENT DESCRIPTION 21 Add 6 drops of HI 83742B 0 Folin Ciocalteu s Reagent to the cuvet Replace the cap and shake gently to mix x 6 Wait for 1...

Page 9: ...replaced 20 TOTAL PHENOLS WHITE WINE SPECIFICATIONS SPECIFICATIONS SPECIFICATIONS SPECIFICATIONS SPECIFICATIONS Range 0 000 to 0 750 g L Resolution 0 001 Precision 0 015 0 350 g L Light Source Tungste...

Page 10: ...e cuvet for reference zero and measurement Inverted the sample and the zero cuvet are inverted b on sample reading b on sample reading b on sample reading b on sample reading b on sample reading Indic...

Page 11: ...hen keeping the syringe in vertical position above the cuvet push the plunger completely down into the syringe Now the exact amount of 5 mL has been added to the cuvet GENERAL TIPS FOR AN ACCURATE MEA...

Page 12: ...nual are referred to 20 C 68 F As a general rule of thumb they should be doubled at 10 C 50 F and halved at 30 C 86 F 17 MEASUREMENT MEASUREMENT MEASUREMENT MEASUREMENT MEASUREMENT Press RANGE to sele...

Page 13: ...nm Method Direct reading REQUIRED REAGENTS REQUIRED REAGENTS REQUIRED REAGENTS REQUIRED REAGENTS REQUIRED REAGENTS Code Description Quantity test HI 83742 0 Wine Solvent 1 8 mL HI 83742 3 Wine Solven...

Page 14: ...the zero zero zero zero zero Insert the zero cuvet into the holder and close the lid Press ZERO and will blink on the display SAMPLE PREPARATION SAMPLE PREPARATION SAMPLE PREPARATION SAMPLE PREPARATIO...

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