Hanna Instruments HI 83742 Instruction Manual Download Page 11

11

The instructions listed below should be carefully followed during testing to ensure best accuracy.

• For a correct filling of the cuvet: the liquid in the

cuvet forms a convexity on the top; the bottom of
this convexity must be at the same level of the
10 mL mark.

• For dosing the wine sample, we recommend to

use the supplied Hanna automatic pipettes
(HI 731340 - 200 µL, HI 731342 - 2000 µL).
For a correct use of the Hanna automatic pipette,
please follow the related Instruction Sheet.

• In order to avoid reagent leaking and to obtain

more accurate measurements, it is recommended

to close the cuvet first with the supplied HDPE
plastic stopper 

and then with the black

cap.

• In order to measure exactly 5 mL of reagent with the 5 mL syringe:

(a) push the plunger completely into the syringe and insert the tip into the reagent bottle.
(b) pull the plunger up until the lower edge of the seal is exactly on the 5 mL mark.
(c) take out the syringe and clean the outside of the syringe tip. Be sure that no drops are

hanging on the tip of the syringe, if so eliminate them. Then, keeping the syringe in
vertical position above the cuvet, push the plunger completely down into the syringe. Now
the exact amount of 5 mL has been added to the cuvet.

GENERAL TIPS FOR AN ACCURATE MEASUREMENT

Hanna
automatic pipette

(a)

(b)

(c)

10 mL

18

COLOR HUE

SPECIFICATIONS

SPECIFICATIONS

SPECIFICATIONS

SPECIFICATIONS

SPECIFICATIONS
Range

0.00 to 9.99

Resolution

0.01

Precision

±0.03 @ 0.75

Light Source

Tungsten lamp with narrow band interference filters @ 420 and 520 nm

Method

Direct reading.

REQUIRED REAGENTS

REQUIRED REAGENTS

REQUIRED REAGENTS

REQUIRED REAGENTS

REQUIRED REAGENTS

Code

Description

Quantity/test

HI 83742-0

Wine Solvent-1

8 mL

For reagent set and other accessories see page 26.

MEASUREMENT

MEASUREMENT

MEASUREMENT

MEASUREMENT

MEASUREMENT

• Press “RANGE” to select the parameter code “P3” for

color hue (see page 13).

SAMPLE PREPARATION

SAMPLE PREPARATION

SAMPLE PREPARATION

SAMPLE PREPARATION

SAMPLE PREPARATION

• Replace the cap. This is the sample.

#2

(Sample)

• Use the 2000 µL automatic pipette to add exactly

2 mL of wine sample to an empty cuvet. For a correct
use of the automatic pipette please follow the related
Instruction Sheet.

• Then use the 1 mL plastic pipette to fill the cuvet

with HI 83742-0 Wine Solvent-1 up to the 10 mL
mark.

10 mL

Summary of Contents for HI 83742

Page 1: ...Instruction Manual HI 83742 COLOR PHENOLS ISM for wine analysis w w w h a n n a i n s t c o m This Instrument is in Compliance with the CE Directives MAN83742R3 04 06 w w w h a n n a i n s t c o m...

Page 2: ...tampering or lack of prescribed maintenance are not covered If service is required contact your dealer If under warranty report the model number date of purchase serial number and the nature of the f...

Page 3: ...hazardous area The blinking will appear when the batteries power gets low When batteries are completely discharged 0 bAtt will appear and after two seconds the instrument is switched off Remove the b...

Page 4: ...ecommends usingthespecialwinesolventtominimizepossibleerrorsduetodilution Phenoldeterminationofwine Phenoliccompoundsareimportantforseveralreasonssincethey i affectthecolorofthewine ii have anastringe...

Page 5: ...ungstenicacid H3PW12O40 andphosphomolybdenicacid H3PMo12O40 to thequinoidform R O TheconcomitantreductionoftheFolin Ciocalteureagentcausesabluecolorin thesamplethatisproportionaltothetotalphenoliccont...

Page 6: ...s colorimeters is based on special subminiature tungsten lamps and narrow band interference filters to guarantee both high performance and reliable results Block diagram optical layout 23 TOTAL PHENOL...

Page 7: ...This to avoid measurement interference due to unwanted reflection and absorption of light It is recommended not to touch the cuvet walls with hands Furthermore in order to maintain the same conditions...

Page 8: ...to 20V DC 2 5 Watt FUNCTIONAL DESCRIPTION FRONT REAR INSTRUMENT DESCRIPTION 21 Add 6 drops of HI 83742B 0 Folin Ciocalteu s Reagent to the cuvet Replace the cap and shake gently to mix x 6 Wait for 1...

Page 9: ...replaced 20 TOTAL PHENOLS WHITE WINE SPECIFICATIONS SPECIFICATIONS SPECIFICATIONS SPECIFICATIONS SPECIFICATIONS Range 0 000 to 0 750 g L Resolution 0 001 Precision 0 015 0 350 g L Light Source Tungste...

Page 10: ...e cuvet for reference zero and measurement Inverted the sample and the zero cuvet are inverted b on sample reading b on sample reading b on sample reading b on sample reading b on sample reading Indic...

Page 11: ...hen keeping the syringe in vertical position above the cuvet push the plunger completely down into the syringe Now the exact amount of 5 mL has been added to the cuvet GENERAL TIPS FOR AN ACCURATE MEA...

Page 12: ...nual are referred to 20 C 68 F As a general rule of thumb they should be doubled at 10 C 50 F and halved at 30 C 86 F 17 MEASUREMENT MEASUREMENT MEASUREMENT MEASUREMENT MEASUREMENT Press RANGE to sele...

Page 13: ...nm Method Direct reading REQUIRED REAGENTS REQUIRED REAGENTS REQUIRED REAGENTS REQUIRED REAGENTS REQUIRED REAGENTS Code Description Quantity test HI 83742 0 Wine Solvent 1 8 mL HI 83742 3 Wine Solven...

Page 14: ...the zero zero zero zero zero Insert the zero cuvet into the holder and close the lid Press ZERO and will blink on the display SAMPLE PREPARATION SAMPLE PREPARATION SAMPLE PREPARATION SAMPLE PREPARATIO...

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