56
oPeration
|
PLC 2050/2250/2500 PurifiCation SyStemS
LO
O
p
IN
je
C
tI
O
N
w
It
h
m
a
N
U
a
L V
a
LV
e
4. insert the syringe with the needle into the needle port and load the sample into the
sample loop. any surplus will flow through the waste tubing (Port 6).
5. Switch the valve from the LoaD to inJeCt position with a 60° rotation of the handle.
●
the sample is pumped into the fluid path and pushed into the chromatography column
via the mobile phase used.
●
When the injection mode
wait for Injection
is selected in the method edition (refer to
the
Gilson Glider Prep Software (GGP) User’s Guide
), the elution phase will start upon
user confirmation that the valve has been manually switched.
COLU
MN
LOAD
P
W
W
3
4
5
1
2
6
COLU
MN
INJ
P
W
W
3
4
5
1
2
6
Figure 54
Manual Injection - Load and Inject Diagrams
Figure 55
Injection Mode Setting - Wait for Injection Message
