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Gilson PLC 2050, User Manual

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51

PLC 2050/2250/2500 PurifiCation SyStemS 

| uSer’S GuiDe

Chapter 3

Chapter 3 | 

OperatION

IN thIS Chapter

 

●

Start up

 | 

52

 

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Prime the Pump

 | 

53

 

●

Loop injection with automatic Valve

 | 

54

 

●

Loop injection with manual Valve

 | 

55

 

●

Power Down

 | 

57

Summary of Contents for PLC 2050

Page 1: ...PLC 2050 2250 2500 Purification Systems User s Guide...

Page 2: ...l product and company names are trademarks or registered trademarks of their respective holders Use of the trademark s in this document does not imply any affiliation with or endorsements by the trade...

Page 3: ...ns of Damage 10 Site Requirements 10 Spacing 10 Storage and Movement 10 CHAPTER 1 Introduction 11 Description 12 Unpack 15 Customer Service 19 Terms and Abbreviations 20 Technical Specifications 21 De...

Page 4: ...ER 4 Maintenance 59 Helpful Hints 60 Maintenance Schedule 61 Replace a Pump Seal 62 Clean the Pump Pistons 67 Replace the Mixing Chamber Piston 68 Test and Repair the UV VIS Detector 69 CHAPTER 5 Trou...

Page 5: ...of VERITY 4020 Single Syringe Pump 101 APPENDIX E PLC with MS Detector Configuration 103 Unpack and Place the System 104 Control Software 104 Installation 104 Electrical and Gas Connections 106 Plumb...

Page 6: ...PLC 2050 2250 2500 Purification Systems USER S GUIDE...

Page 7: ...rvice and repair of the instrument Failure to comply with these precautions or with warnings described in the user s guide violates safety standards of design manufacture and intended use of the instr...

Page 8: ...pear on the instrument or in this document SYMBOL EXPLANATION Direct Current Alternating Current Protective Conductor Terminal Electrical Power ON O Electrical power OFF Caution Caution Risk of Electr...

Page 9: ...Administration United States Electrical Hazards Unless specifically instructed do not remove any protective covers Detach all sources of voltage from the instrument before the service repair or exchan...

Page 10: ...to Technical Specifications on page 21 for more details Spacing Allow sufficient spacing around the system for proper cooling and for the connection of power cords plumbing injection pump liquid hand...

Page 11: ...50 2500 Purification Systems USER S GUIDE Chapter 1 Chapter 1 INTRODUCTION IN THIS CHAPTER Description 12 Unpack 15 Customer Service 19 Terms and Abbreviations 20 Technical Specifications 21 Detailed...

Page 12: ...diagrams provide a general description of the PLC Purification System with standard components Figure 1 PLC 2050 Purification System with Standalone Column Holder Prime and Purge Valve and Pump Heads...

Page 13: ...fer to page 46 for installation details Backflush Valve Refer to page 30 for a detailed description Standalone Column Holder Not Shown Refer to page 47 for installation instructions Funnel Rack Not Sh...

Page 14: ...ngle stroke The syringe pump enables automatic unattended injections on PLC Purification Systems allowing users to purify more compounds with less manual interaction For more information refer to the...

Page 15: ...ately 65 kg 143 lbs and an additional 10 kg 22 lbs with packaging The system is too heavy to be lifted or moved by one person safely To avoid personal injury and for general safety if moving or liftin...

Page 16: ...SS ballast at one end with colored cable ties for identification PEEK nuts and ETFE ferrules for 3 16 tubing Waste outlet 1 8 OD 2 4 mm ID 2 m qty 1 ETFE or PTFE with an SS ballast at one end SS fitt...

Page 17: ...w USB drive qty 1 Documentation Control software installation file Documentation PLC 2050 2250 2500 Purification Systems User s Guide Gilson Glider Prep GGP Software User s Guide UV VIS Detector Servi...

Page 18: ...1 8 tubing special screws and cable Control module power supply bracket and cables Tubing kit 1 mm ID PLC 2050 or 2 1 mm ID PLC 2250 and PLC 2500 Quaternary gradient system 1 8 PLC 2050 and PLC 2250 o...

Page 19: ...500 Standalone column holder for LC and flash columns qty 1 ETFE or PTFE tubing kit 1 8 OD VERITY 4020 Syringe Pump with GX 241 Liquid Handler qty 1 VERITY 1910 MS Detector System qty 1 VERITY 1910 MS...

Page 20: ...light scattering detector PEEK Polyether ether ketone EMC Electromagnetic compatibility PFA Perfluoroalkoxy alkane EPI Equipment de protection individuelle PLC Preparative liquid chromatography ETFE...

Page 21: ...ions available Refer to Fraction Collector on page 84 in the Replacement Parts and Accessories appendix Rack overlays for easy tube identification optional Refer to Install the Rack Overlays on page 4...

Page 22: ...24 4 x 23 2 x 26 in Electrical Protection General Delayed action fuses 6 3A H 250 V T type qty 2 24 V and 5 V Delayed action fuses with different ratings L 250 V T type Environmental Conditions SPECIF...

Page 23: ...Hastelloy C Pump pistons Zirconium oxide Pump check valves PCTFE ruby and sapphire Purge valve Ketron CA30 PEEK and Kalrez Tubing fittings sample loops and unions 316 SS PEEK ETFE PTFE PFA and FEP UV...

Page 24: ...Flow Rate Performance Accuracy 2 with H2O degassed at 20 C Repeatability Better than 0 5 RSD Safety and Compliance The instrument has been certified to safety standards specified for Canada Europe and...

Page 25: ...leys belts and a stepper motor which drives the cams and pistons For plumbing connections refer to the table on page 26 A manual valve at the outlet black knurled knob allows users to easily purge or...

Page 26: ...C 2500 K ETFE junction tubing for cleaning discs C1 PEEK nuts Long for 1 8 tubing PN 21040195 for PLC 2050 and PLC 2250 Standard for 3 16 tubing PN 49040132 for PLC 2500 L Outlet check valve housings...

Page 27: ...modified by replacing the chamber piston Refer to Replace the Mixing Chamber Piston on page 68 Refer to the diagram below for components and connections to the dynamic mixing chamber Dynamic Mixing Ch...

Page 28: ...and PLC 2250 PN 21040175 for PLC 2500 is also available For plumbing connections refer to Gradient Valve to Pump Heads on page 39 Binary and Quaternary Low Pressure Valves PART NUMBER BINARY VALVES P...

Page 29: ...2050 SS preformed tubing 1 8 OD 2 1 mm ID PN 21040120 for PLC 2250 and PLC 2500 F Sample loop preformed SS 1 8 OD 2 1 mm ID Refer to Sample Loops on page 85 for a complete listing B Dynamic mixing ch...

Page 30: ...sample can be manually injected into the column by switching the valve BACKFLUSH VALVE The four way automatic backflush valve allows users to change the pumping direction elution mode into the connect...

Page 31: ...by default standard UV dual wavelength 254 280 nm by default optional UV detector four wavelength DAD 200 400 nm optional UV VIS detector four wavelength DAD 200 600 nm optional UV VIS detector 4 wav...

Page 32: ...flow cell Racks The integrated collector is provided with three SS racks standard PN 21040019 designed for 18 mm diameter tubes accommodating up to 192 tubes and a maximum volume of 6 1 L Refer to Fra...

Page 33: ...e direct data transfer to a private server ANALOG SIGNAL INPUTS The analog input connectors red black can be used for signal acquisition of additional detectors 1 V 1V A 10 bit A D converter on an ele...

Page 34: ......

Page 35: ...components should be set up and installed in the following order Complete instructions for each step are included in this chapter IN THIS CHAPTER Place the Solvent Bottle Tray 36 Plumbing Connections...

Page 36: ...ach inlet line place the ETFE ferrule into the PEEK body of the gradient valve and screw on the PEEK nut finger tight and slide the spring to place its end in the knurled part of the PEEK nut Springs...

Page 37: ...BING CONNECTIONS AND COMPONENTS DIAGRAM Refer to the following diagram and table before connecting the inlet tubing A B C D E F H F K A1 1 B1 1 C1 D1 G F M N K L Figure 26 Component and Connection Dia...

Page 38: ...C 2500 K SS ballast E Plug PN 49041019 for PLC 2050 and PLC 2250 PN 21040196 for PLC 2500 L Solvent bottle tray F PEEK nut PN 21040194 short and PN 21040195 long for PLC 2050 and PLC 2250 PN 49040132...

Page 39: ...e outlet on the left side of the PLC Purification System 3 Finger tighten the three fittings and then ensure that the three fittings on the tee union are fully tightened Waste Outlet Tubing The waste...

Page 40: ...stems come with the sample loop pre installed at the factory however for custom orders please follow the instructions below To make the sample loop connection Connect a sample loop to Port 3 and Port...

Page 41: ...shut off valve outlet and finger tighten the PEEK nut The shut off valve is connected to the port 2 of the automatic loop injection valve Column Setup A wide range of columns can be used with PLC Pur...

Page 42: ...TE If additional valves are installed such as the automatic backflush valve PN 21040005 for PLC 2050 PN 21040006 for PLC 2250 and PLC 2500 or manual column switching valve PN 21040013 for PLC 2050 PN...

Page 43: ...ble To install the racks 1 Place the tubes in the rack holes and then place the racks on the collector plate 2 Locate the middle slot in the rear of the rack Slide the slot over the back positioning g...

Page 44: ...y A voltage selector located near the power receptacle allows users to adjust the input voltage of the system Check that the position of the voltage selector corresponds to the voltage of the mains To...

Page 45: ...to disconnect it from mains supply at any time In the event of an emergency the power connector of the instrument must be easily accessible and removable The system must never be operated from a powe...

Page 46: ...ed with a 3 mm Allen wrench 2 Connect the manual column switching valve inlet Port 1 to the loop injection valve outlet 3 Connect the column switching valve outlet Port 4 to the bulkhead union detecto...

Page 47: ...odule for Additional Detectors The system is equipped with a built in analog input to acquire the signal from an additional detector To improve the quality of the external detection and increase the n...

Page 48: ...Rack Overlays Rack overlays for 18 16 mm tubes and 13 12 mm tubes are designed for glass tubes identification They correspond to the default collection probe trajectory called Alternating Vertical in...

Page 49: ...the standard racks To install the funnel rack 1 Use a 2 5 mm Allen wrench to remove the two cylindrical head screws from the front positioning guide 2 Remove the front positioning guide from the tray...

Page 50: ......

Page 51: ...0 2250 2500 Purification Systems USER S GUIDE Chapter 3 Chapter 3 OPERATION IN THIS CHAPTER Start Up 52 Prime the Pump 53 Loop Injection with Automatic Valve 54 Loop Injection with Manual Valve 55 Pow...

Page 52: ...rive Activity light flashes to indicate that the storage device is being used The screen switches on and displays the Microsoft Windows logo After one minute the Windows desktop appears 2 Wait for Gil...

Page 53: ...e Mode in GGP Software Refer to the Gilson Glider Prep Software GGP User s Guide 2 Loosen the knurled knob one half turn counter clockwise of the purge valve located above the pump heads 3 Once solven...

Page 54: ...ivated To inject the sample in the column 1 Fill a syringe with the desired volume of sample 2 Place the syringe in the Luer female fitting in the top position of the injection valve Port 1 3 Load the...

Page 55: ...n the valve is turned from the INJECT to LOAD position NOTICE Rinse the valve with water after using buffer solutions to prevent crystals from forming which can cause scratches on the rotor seal of th...

Page 56: ...handle The sample is pumped into the fluid path and pushed into the chromatography column via the mobile phase used When the injection mode Wait for Injection is selected in the method edition refer...

Page 57: ...nd the running method 2 Close the control software 3 Turn off the PC and wait for the touchscreen to turn off The UV VIS detector lamp will remain on 4 Switch the main power switch to 0 Refer to Exter...

Page 58: ......

Page 59: ...use good laboratory practice GLP including but not limited to wearing protective clothing and preparing the maintenance space for service After completing the maintenance operation verify the safe an...

Page 60: ...ccidentally spilled on the instrument wipe it up immediately If a contamination is suspected the external surfaces of the instrument should be wiped down with 10 aqueous IPA to remove any residue Flui...

Page 61: ...ed Replace the detector deuterium lamp when efficiency is unreliable As needed Check the suitable tightening of fittings and the proper state of tubing not kinked damaged etc Perform maintenance any t...

Page 62: ...ronment There are four seals one for each pump head and one for each cleaning disc To disassemble a pump head with cleaning disc and to replace a seal follow the instructions below Remove the Pump Hea...

Page 63: ...screws 7 Carefully remove the pump head The piston and the cleaning disc PEEK part will be visible 8 Carefully remove the cleaning disc to fully expose the piston NOTE If needed disconnect the ETFE j...

Page 64: ...orn seal particles and then plunge the new seal in IPA for lubrication 2 Correctly position the new seal on the pump head or cleaning disc 3 Use the special tool A and B pictured below 4 Position the...

Page 65: ...ressure fluctuations and irregular flow There are four check valves two per head one for each inlet and one for each outlet To clean or replace a check valve follow the instructions below 1 Purge the...

Page 66: ...w is always directed upwards 7 Tighten the valve housing in the pump head with a 17 mm spanner until you hear a little crunch which means that the new check valve is properly seated This noise corresp...

Page 67: ...ypically used when aqueous buffers are pumped and function to manually wash the buffers from the back of the seals and to prevent early wear of these seals 1 Fit the 1 8 PEEK nuts and ETFE ferrules su...

Page 68: ...hamber piston to ensure better mixing 1 Purge the system with a suitable solvent 2 Switch off the system and disconnect it from the mains 3 Disconnect the SS tubing from the chamber outlet with a 3 8...

Page 69: ...sions up to 800 nm a secondary halogen lamp is used The efficiency of the lamp and the cleanliness of the flow cell must be checked periodically If efficiency of detection is unreliable detector lamp...

Page 70: ......

Page 71: ...71 PLC 2050 2250 2500 Purification Systems USER S GUIDE Chapter 5 Chapter 5 TROUBLESHOOTING IN THIS CHAPTER Troubleshooting Table 72 Repair and Return Policies 77...

Page 72: ...fitting The fluid path is partially or totally blocked Tighten the fittings and check the state of the tubing Replace the fittings and or the tubing if damaged Clean the fluid path Refer to page 60 Th...

Page 73: ...chamber piston seal if damaged Refer to page 68 Leakage near a high pressure valve The fittings are not sufficiently tightened Improper state of tubing or fitting The fluid path is partially or totall...

Page 74: ...he light intensity curve Refer to the UV VIS Detector Service Guide No signal during UV acquisition UV detector lamp does not switch on Worn UV deuterium lamp Replace the UV deuterium lamp and perform...

Page 75: ...te and cleanliness of collector sensors Fraction collector does not collect properly into tubes Wrong racks used Wrong racks positioning Wrong collection parameters Check rack code in the software mat...

Page 76: ...ep Software GGP User s Guide The touchscreen PC does not turn on at the same time the PLC Purification System turns on An unexpected shutting down of the PC or the system General outage Manually reboo...

Page 77: ...tive Non Warranty Repair For out of warranty repairs contact your local Gilson representative who will discuss service options with you and can assist in making arrangements to return the equipment if...

Page 78: ......

Page 79: ...rrect AC power supply Failure due to mechanical force applied to the unit Failure due to improper handling by the user or an individual not authorized to operate the equipment Failure due to the use o...

Page 80: ...ositioning Valves TERMS OF USE Normal tightening of fittings Use of clean solvents and containers Following the preventative maintenance schedule DAMAGES NOT COVERED Damaged threads or crushed interna...

Page 81: ...ms listed must only be supplied by Gilson or an agent thereof Use of alternative parts may lead to improper operation of the system or failure to comply with safety or EMC regulations NOTICE The manuf...

Page 82: ...r adapter ETFE female to 1 4 28 male 21040202 Luer adapter male with lock to 1 4 28 female 490410675 Luer adapter male with lock to 1 4 28 male 49060022 Reducer 5 16 24 to 10 32 1 mm bore 21040201 Tee...

Page 83: ...00 Ferrule SS for 1 8 tubing qty 10 21040194 Nut PEEK short for 1 8 tubing 21040195 Nut PEEK long for 1 8 tubing 49040132 Nut PEEK for 3 16 tubing 49041123 Nut black PEEK for 1 4 tubing 21040197 Nut S...

Page 84: ...21040026 Rack set 13 x 73 mm hemolysis tubes 21040191 Rack set 12 x 65 mm tubes 21040027 Rack funnel 16 outlets 21040028 Rack overlays 18 mm tubes 21040029 Rack overlays 13 mm tubes 21010189 Test tub...

Page 85: ...c 14 mm PLC 2500 21040107 Piston 4 5 x 65 mm PLC 2050 21040108 Piston 10 x 65 mm PLC 2250 21040109 Piston 14 x 65 mm PLC 2500 21040114 Piston seal for mixing chamber PTFE 21040104 Seal 4 5 mm PLC 2050...

Page 86: ...119 Preformed transducer to mixer PLC 2250 2500 21040120 Preformed mixer to injection valve PLC 2250 2500 21040121 Preformed right head to transducer PLC 2050 21040122 Preformed left head to transduce...

Page 87: ...Manual column selection with tubing kit PLC 2250 2500 21040181 Manual injection with tubing kit PLC 2050 21040182 Manual injection with tubing kit PLC 2250 2500 21040172 Shut off injection valve outle...

Page 88: ......

Page 89: ...hemicals The glass may be clear or translucent in which case it is often referred to as fused quartz The glass has very high viscosity and this property allows the glass to be formed cooled and anneal...

Page 90: ...nd stiffness of PEEK and dramatically lowers its expansion rate It offers designers optimum wear resistance and load carrying capability in a PEEK based product This grade provides more thermal conduc...

Page 91: ...m oxides practically pure for the sapphire 99 99 Al2O3 The color of the ruby is produced by adding a few ppm parts per million of chromium oxide CrO3 Synthetic rubies and sapphires have a hexagonal rh...

Page 92: ...the nature of this metal valves made of titanium typically have a shorter lifetime than HPLC grade stainless steel or Hastelloy Zirconium Oxide The zirconium oxide or zirconia ZrO2 has a tetragonal cr...

Page 93: ...ack and Place the System 94 Plumb the Instruments 94 Connect the Transfer Tubing 94 Make the Communication and Power Connections 94 Install the Racks 94 Control Software 94 Installation 95 Plumbing Co...

Page 94: ...iquid Handler User s Guide 2 Make all plumbing connections described in the Installation chapter of this user s guide Connect the Transfer Tubing Connect the transfer tubing as described in Transfer T...

Page 95: ...he liquid handler to the left of the PLC Purification System 3 Place the 3 x 100 mL solvent reservoir rack PN SPL 2334 HDW which must be installed adjacent to the rinse station 4 Set up the VERITY 402...

Page 96: ...Plumbing Connections Connection Tubing Fittings A FROM Reservoir Solvent inlet tubing assembly 3 mm OD 2 mm ID PN 499484021 20 L filter PN 4957226 TO Syringe pump inlet PEEK B FROM Syringe pump outle...

Page 97: ...model and connected column Varies based on PLC model and connected column TO Column F FROM Port 3 Automated sample loop injection valve Sample loop tubing 5 16 24 SS 1 8 OD 1 mL PN 21040030 2 mL PN 21...

Page 98: ...To install the transfer tubing 1 Connect one end with 1 4 28 fitting to the transfer tubing side of the valve of the VERITY 4020 Syringe Pump right hand port and finger tighten the nut 2 Connect the o...

Page 99: ...er outlet G Unit ID GX 241 Liquid Handler The GX 241 Liquid Handler is shipped configured for RS 232 communication with a unit ID of 8 which is the supported configuration in the PLC Purification Syst...

Page 100: ...isconnect pull back on the locking collar and then disconnect the cable from the rear panel of the GX 241 Liquid Handler Locate the appropriate power cord for the line voltage and then make the connec...

Page 101: ...ation System NOTE It is impossible to dispense to the reservoir When aspirating from the probe it is important to take into account the volume of transfer tubing connecting the pump outlet to the prob...

Page 102: ......

Page 103: ...Systems USER S GUIDE Appendix E Appendix E PLC WITH MS DETECTOR CONFIGURATION IN THIS CHAPTER Unpack and Place the System 104 Control Software 104 Installation 104 Electrical and Gas Connections 106 P...

Page 104: ...below for details on placing the system Control Software Control of the PLC Purification System with VERITY 1910 MS Detector and MiDas pump is via Gilson Glider Prep GGP Software accessed via the touc...

Page 105: ...avoid personal injury and for general safety if moving or lifting the VERITY 1910 MS Detector always get another person to assist you Always follow local health and safety regulations The maximum wei...

Page 106: ...libration and ports for PC peripherals P2 D I O USB B Type Connector H1 USB A Type Connector or H2 H G H H A B C D E F H G J L K N P1 Q M G D P1 N G G P3 P3 PLC Purification System MiDas VERITY 1910 M...

Page 107: ...ort Description A PS 2 Keyboard J Analog In B PS 2 Mouse K Analog Out C VGA Monitor L Microphone In D RS 232 for calibration M Gas E eSATA not used N Exhaust F HDMI P Power P1 VERITY 1910 MS Detector...

Page 108: ...at is connected to the VERITY 1910 MS Detector through the rear panel must itself also provide double reinforced insulation to the mains supply Make the following rear panel connections in sequence Re...

Page 109: ...a purity greater than or equal to 99 5 In addition an in line gas filter can be used to trap common contaminants found in labs Gas supply tubing to the instrument should be 6 mm or 1 4 OD PTFE supplie...

Page 110: ...g a split flow interface SFI set up with the correct split ratio The outlet from the column is directed to the splitter module in the MiDas where the fluid stream is split between the integrated UV de...

Page 111: ...Power on the MS detector by pressing the ON OFF power button Y on the front panel The green LED status indicator on the front panel of the MS detector illuminates hardware interlock engaged The green...

Page 112: ......

Page 113: ...ER Technical Specifications 114 Description 115 Make the Rear Panel Connections 116 Install the Nebulizer and Nebulization Chamber 119 Front Panel Connections 120 Install the Chamber Shield 122 Operat...

Page 114: ...ate Up to 30 Hz Detection Photodiode with high sensitivity Filter Moving average Gain setting Dynamic gain management SAGA SEDEX Automated Gain Adjustment Gas requirements For oil free dry and filtere...

Page 115: ...ion such as UV it is not dependent on the absorption of radiation nor do the absorption characteristics of the solvent affect detection Thus solvents which absorb UV radiation can be used As the solve...

Page 116: ...fication Systems PLC with Integrated ELSD Configuration Make the Rear Panel Connections Rear Panel Diagram The rear panel provides connections for a gas inlet and exhaust outlet The exhaust outlet eva...

Page 117: ...rmit gas to leak into the laboratory The gas supply must include a pressure gauge that is stable and regulated by an external manometer The ELS detector is typically operated with a gas pressure of 2...

Page 118: ...phon or to prevent liquid from spilling into the evaporation tube Avoid loops or bends in the black exhaust tubing to avoid condensation traps that can result in poor measurement results Negative pres...

Page 119: ...amber should be pushed as far as possible 4 Rest the nebulization chamber on its inclined support and then tighten the small black nut at the bottom 5 Place the nebulizer on the top of the nebulizatio...

Page 120: ...ste NOTICE Ensure that the waste container is directly below the siphon outlet and that there are no loops or bends in the drain tubing Affix the drain tubing to the inlet of the waste container so th...

Page 121: ...detector while the remainder flows to the UV VIS detector and then on to the fraction collector Use the supplied 1 16 OD PEEK tubing to connect the splitter outlet to the ELS detector nebulizer taking...

Page 122: ...ld diagonally downward To install the chamber shield slide the chamber shield into the exposed notches of the two shield holders Operation Start Up Make all rear and front panel connections before sta...

Page 123: ...hat the end of the tubing is never submered in the liquid Drain tubing that is bent or immersed in the waste container will create pressure fluctuations in the ELS detector and result in poor measurem...

Page 124: ...lly labile or semi volatile a lower temperature could be used to improve the sensitivity by reducing the thermal decomposition or evaporation For a given flow rate and solvent there is however a point...

Page 125: ...n max using the 1 16 OD 0 005 ID 35 cm PEEK splitter tubing Split Flow Rates and Ratios with Acetonitrile ACN ELUTION FLOW RATE mL min SPLIT FLOW RATE IN ELSD mL min SPLIT RATIO 5 0 08 1 62 10 0 29 2...

Page 126: ...tector After each run and before shutting down the PLC Purification System the ELS detector should be cleaned to ensure good performance Follow the preventative maintenance steps below to clean the EL...

Page 127: ...t using a backpressure loop instead of a column Alternatively the light source may need to be replaced or the nebulizer could be obstructed 6 A decrease in the sensitivity is often caused by the nebul...

Page 128: ...C Purification System 2 Disconnect the PLC Purification System from the mains 3 Stop the flow of gas to the PLC Purification System 4 Remove the chamber shield 5 Disconnect the nebulizer liquid inlet...

Page 129: ...is working properly NOTICE Replace the nebulizer if the cleaning and preventative maintenance steps provided are ineffective Decontaminate the Nebulizer Set the evaporation temperature to 95 C and the...

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Page 132: ...www gilson com ContactUs LT380160 01...

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