8
PIPETMAN
®
P
6 - GeneraL GUIdeLInes For Good
PIPettInG
Make sure that you
operate the push-
button slowly and
smoothly.
When aspirating,
keep the tip at a
constant depth
below the surface
of the liquid (refer
to the table).
Change the tip before aspirating a different liquid,
sample, or reagent.
Change the tip if a droplet remains at the end of
the tip from the previous pipetting operation.
Each new tip should be pre-rinsed with the liquid
to be pipetted.
Liquid should never enter the tip-holder; to
prevent this:
- press and release the push-button slowly and
smoothly,
- never turn the pipette upside down,
- never lay the pipette on its side when there is
liquid in the tip.
If you use the same tip with a higher volume,
pre-rinse the tip.
For volatile solvents you should saturate the
air-cushion of your pipette by aspirating and
dispensing the solvent repeatedly before
aspirating the sample.
When pipetting liquids with temperatures
different to the ambient temperature, pre-rinse
the tip several times before use.
You may remove the tip-ejector (
see Chapter 11 -
Maintenance
) to aspirate from very narrow tubes.
Table - Immersion Depth
and Wait Time
Model
Immersion Wait Time
Depth (mm) (seconds)
P2
1
1
P10
1
1
P20
2-3
1
P100
2-4
1
P200
2-4
1
P1000
2-4
2-3
P5000
3-6
4-5
P10mL
5-7
4-5
Summary of Contents for PIPETMAN P Series
Page 23: ...23 notes...
