GenScript eBlot User Manual Download Page 3

Product Contents 

Product Contents,

 

continued

Type of products

eBlot

TM

 Protein Transfer 

Device Contents

Upon Receiving 

the Instrument

eBlot

TM

 Protein Transfer 

Pads 

Component

e

Blot

TM

 Protein Transfer Device

e

Blot

TM

 Graphite Electrode (Installed inside the device)

Sponge Cushion (Installed inside the device) 

Regional specific power cord 

Forceps

Shovel

Shallow Tray

Quantity

1 each

1 each

2 each

1 each

1 each

1 each

2 each

Product

e

Blot

TM

 Protein Transfer Pads

(Basic, without membrane, 20-pak)

e

Blot

TM

 Protein Transfer Pads (Nitrocellulose, 20-pak)

e

Blot

TM

 Protein Transfer Pads (PVDF, 20-pak)

Cat. No.

L03011

L03013

L03014

This manual is supplied with the 

e

Blot

TM

 Protein Transfer Device (L03010).

Components for 

e

Blot

TM

 Protein Transfer Pad are as follows:

The contents of the 

e

Blot

TM

 Protein Transfer Device are listed below

Examine the unit carefully for any damage incurred during transit. File any 

damage claims with the carrier. The warranty does not cover in-transit

damage.

The following 

e

Blot

TM

 Protein Transfer Pads are available from GenScript:

Component

e

Blot

TM

 Protein Transfer Pad

Nitrocellulose membranes (20 sheets)

PVDF membranes (20 sheets)

e

Blot

TM

 Equilibration Buffer (125 ml) 

Gel Window (66 mm×56 mm)

Gel Window (76 mm×64 mm)

Gel Window (88 mm×78 mm)

Absorbent Filter Paper

Sponge Cushion

Basic

20

-

-

2

1

1

1

1

2

PVDF

20

-

1

2

1

1

1

1

2

Nitrocellulose

20

1

-

2

1

1

1

1

2

The 

e

Blot

TM

 Protein Transfer Pads come with the following components. 

eBlot

TM

 Graphite Electrode

eBlot

TM

 Equilibration Buffer

The 

e

Blot

TM

 Graphite Electrode (L03012) is available separately from GenScript:

For best results, when a graphite electrode has been used for 100 times of 

protein transfer, replace it with a new one. 

The 

e

Blot

TM

 Equilibration Buffer (M01078, 125 ml) is available separately from 

GenScript.

Store the 

e

Blot

TM

 Protein Transfer Pads at room temperature. For best results, 

use the 

e

Blot

TM

 Protein Transfer Pads before the expiration date printed on the 

package.  

Component

1× 

e

Blot

TM

  Cathode Pad

1× 

e

Blot

TM

  Anode Pad

e

Blot

TM

 Protein Transfer System 

www.genscript.com

e

Blot

TM

 Protein Transfer System 

www.genscript.com

Quantity

1

1

- 2 -

-1 -

DO NOT start a run without  properly assembled Transfer Stacks in place!

  

Important 

Product

e

Blot

TM

 Graphite Electrode

Cat. No.

L03012

Product

e

Blot

TM

 Equilibration Buffer

Cat. No.

M01078

Summary of Contents for eBlot

Page 1: ...iscataway NJ 08854 Tel 732 885 9188 732 885 9688 Fax 732 210 0262 732 885 5878 Email product genscript com Version No 08152014 For fast semi dry electroblotting of proteins from mini polyacrylamide ge...

Page 2: ...ions eBlotTM Protein Transfer Device Accessory Products Introduction Quick Reference Guide Protocols Troubleshooting Examples of Results Technical Support Warranty Appendix 1 3 5 7 8 11 12 21 22 23 24...

Page 3: ...mponent eBlotTM Protein Transfer Pad Nitrocellulose membranes 20 sheets PVDF membranes 20 sheets eBlotTM Equilibration Buffer 125 ml Gel Window 66 mm 56 mm Gel Window 76 mm 64 mm Gel Window 88 mm 78 m...

Page 4: ...ely from GenScript The specifications for eBlotTM Graphite Electrode are listed below eBlotTM Protein Transfer Pads Specifications eBlotTM Anode Pad eBlotTM Cathode Pad Materials 90 mm l 80 mm w 2 5 m...

Page 5: ...both left and right status lights are on the device is working at numbering mode The Reset button is used to clear parameters The Min button is used to shift between transferring and numbering mode a...

Page 6: ...s Equilibration Buffer and Gel Window Each pack of eBlotTM Protein Transfer Pad contains an 1 eBlotTM Cathode Pad and an 1 eBlotTM Anode Pad presoaked with proprietary cathode buffer and anode buffer...

Page 7: ...etary fast semi dry blotting technique developed by GenScript To use the eBlotTM Protein Transfer System for rapid protein blotting assemble the eBlotTM Protein Transfer Pad with your pre run gel and...

Page 8: ...ng the number of uses of the graphite anode Replacement of worn graphite anode Action Connect eBlotTM Device to an electrical outlet and power switch is on Assemble transfer stack on the device and cl...

Page 9: ...e Protocols continued Protocols continued 8 Attach the power cord to the AC inlet and then to the electrical outlet Use only properly grounded AC outlets and power cords 9 When the electrophoresis of...

Page 10: ...nd cathode pad to prevent short circuit Note During assembling of the transfer stack make sure to remove all the air bubbles trapped between the transfer pads pre run gel and the membrane which may pr...

Page 11: ...o perform the replacement Note After about 40 runs take out the graphite electrode soak in distilled water for 30 minutes dry the surface with a clean paper towel then leave air dry overnight with gra...

Page 12: ...phite Electrode out of the device 3 Tear the sealing of a new Graphite Electrode package and take the new Graphite Electrode out of the package Place the new Graphite Electrode into the anode tank and...

Page 13: ...ansfer stack is assembled correctly use the eBlotTM Anode Pad first followed by the membrane the pre run gel Gel Window and eBlotTM Cathode Pad Check the transfer stack and ensure Gel Window covered c...

Page 14: ...vent or sample For any inquiry or request for repair service contact GenScript after confirming the model and serial number of your instrument For your protection items being returned must be insured...

Page 15: ...26 25 Appendix Appendix continued eBlotTM Protein Transfer System www genscript com eBlotTM Protein Transfer System www genscript com...

Page 16: ...27 Appendix continued eBlotTM Protein Transfer System www genscript com...

Reviews: