GenScript eBlot User Manual Download Page 1

Page: 1 / 16

GenScript USA Inc.

860 Centennial Ave.

Piscataway, NJ 08854

Tel: 732-885-9188, 732-885-9688

Fax: 732-210-0262, 732-885-5878

Version No. 08152014

For fast semi-dry electroblotting of proteins from mini polyacrylamide

gels to nitrocellulose or PVDF membranes

Summary of Contents for eBlot

Page 1: ...iscataway NJ 08854 Tel 732 885 9188 732 885 9688 Fax 732 210 0262 732 885 5878 Email product genscript com Version No 08152014 For fast semi dry electroblotting of proteins from mini polyacrylamide ge...

Page 2: ...ions eBlotTM Protein Transfer Device Accessory Products Introduction Quick Reference Guide Protocols Troubleshooting Examples of Results Technical Support Warranty Appendix 1 3 5 7 8 11 12 21 22 23 24...

Page 3: ...mponent eBlotTM Protein Transfer Pad Nitrocellulose membranes 20 sheets PVDF membranes 20 sheets eBlotTM Equilibration Buffer 125 ml Gel Window 66 mm 56 mm Gel Window 76 mm 64 mm Gel Window 88 mm 78 m...

Page 4: ...ely from GenScript The specifications for eBlotTM Graphite Electrode are listed below eBlotTM Protein Transfer Pads Specifications eBlotTM Anode Pad eBlotTM Cathode Pad Materials 90 mm l 80 mm w 2 5 m...

Page 5: ...both left and right status lights are on the device is working at numbering mode The Reset button is used to clear parameters The Min button is used to shift between transferring and numbering mode a...

Page 6: ...s Equilibration Buffer and Gel Window Each pack of eBlotTM Protein Transfer Pad contains an 1 eBlotTM Cathode Pad and an 1 eBlotTM Anode Pad presoaked with proprietary cathode buffer and anode buffer...

Page 7: ...etary fast semi dry blotting technique developed by GenScript To use the eBlotTM Protein Transfer System for rapid protein blotting assemble the eBlotTM Protein Transfer Pad with your pre run gel and...

Page 8: ...ng the number of uses of the graphite anode Replacement of worn graphite anode Action Connect eBlotTM Device to an electrical outlet and power switch is on Assemble transfer stack on the device and cl...

Page 9: ...e Protocols continued Protocols continued 8 Attach the power cord to the AC inlet and then to the electrical outlet Use only properly grounded AC outlets and power cords 9 When the electrophoresis of...

Page 10: ...nd cathode pad to prevent short circuit Note During assembling of the transfer stack make sure to remove all the air bubbles trapped between the transfer pads pre run gel and the membrane which may pr...

Page 11: ...o perform the replacement Note After about 40 runs take out the graphite electrode soak in distilled water for 30 minutes dry the surface with a clean paper towel then leave air dry overnight with gra...

Page 12: ...phite Electrode out of the device 3 Tear the sealing of a new Graphite Electrode package and take the new Graphite Electrode out of the package Place the new Graphite Electrode into the anode tank and...

Page 13: ...ansfer stack is assembled correctly use the eBlotTM Anode Pad first followed by the membrane the pre run gel Gel Window and eBlotTM Cathode Pad Check the transfer stack and ensure Gel Window covered c...

Page 14: ...vent or sample For any inquiry or request for repair service contact GenScript after confirming the model and serial number of your instrument For your protection items being returned must be insured...

Page 15: ...26 25 Appendix Appendix continued eBlotTM Protein Transfer System www genscript com eBlotTM Protein Transfer System www genscript com...

Page 16: ...27 Appendix continued eBlotTM Protein Transfer System www genscript com...