- 20 -
e
Blot
TM
Protein Transfer System
www.genscript.com
- 19 -
e
Blot
TM
Protein Transfer System
www.genscript.com
4. Switch
On
the
e
Blot
TM
Device. Press and hold
Min.
button for 2 seconds
to toggle to numbering mode. When the upper three digits are flashing,
press
Reset
button to zero the transferring times.
Protocols,
continued
Replacing the Graphite
Electrode,
continued
After successfully installing Graphite Electrode into the
e
Blot
TM
Device, you are
ready to use the
e
Blot
TM
Device for another blotting application.
2. Switch
Off
the
e
Blot
TM
Protein Transfer Device. Open the lid of the device
and take the worn Graphite Electrode out of the device.
3. Tear the sealing of a new Graphite Electrode package and take the new
Graphite Electrode out of the package. Place the new Graphite Electrode
into the anode tank and close the lid of the device.
1. If the
e
Blot
TM
Protein Transfer Device works at transferring mode, press and
hold
Min.
button for 2 seconds to toggle to numbering mode. If the upper
three digits show “100” or a number greater than “100”, perform the replacing
protocol as describe below.
Protocols,
continued
During the transfer process, the Graphite Electrode will absorb ions from anode
pad as well as lose carbon composition of the anode buffer. For best blotting
results, after having been used for
100 times
of transfer, the worn Graphite
Electrode should be replaced by a new one.
Replacing the Graphite
Electrode
