- 18 -
e
Blot
TM
Protein Transfer System
www.genscript.com
- 17 -
e
Blot
TM
Protein Transfer System
www.genscript.com
3. At the end of the transfer, current automatically shuts off and the
e
Blot
TM
Protein Transfer Device signals the end of transfer with repeated beeping
sound. The right status light stops flashing and the lower five digits show
text (00:00).
4. Press any button on the control panel to stop the beeping.
5. Proceed to disassemble the stack and clean the device.
To obtain good transfer and detection results, disassemble the transfer stack
right away after ending the blotting procedure.
1. Open the closed lid by pressing the
Open
button.
2. Carefully separate the transferred membrane from the transfer stack and
proceed with further protein detection procedures.
Note: If you are using PVDF membrane, place the membrane immediately into the
blocking solution (or water) as PVDF membrane dries quickly. If the PVDF
membrane is dried, rewet the membrane with methanol and then rinse it with
distilled water to wet it completely before use.
3. Discard the gel and the used
e
Blot
TM
Protein Transfer Pad.
Note: Do not re-use the eBlot
TM
Protein Transfer Pad after blotting. Discard after each
use.
4. Clean the titanium cathode plate, graphite anode plate and its surrounding
area with a dry cloth or paper tissue.
5. Replace the Absorbent Filter Paper in the Waste Tray with a new one when
it has soaked up the waste from 20 times of transfer. Pull out the Waste
Tray from the right side of the device to perform the replacement.
Note: After about 40 runs, take out the graphite electrode, soak in distilled water for 30
minutes, dry the surface with a clean paper towel, then leave air dry overnight with
graphite side up.
At this point, the
e
Blot
TM
Protein Transfer Device is ready for another run.
If you are not using the device, turn off the Power Switch located on the back
of the device.
For any other repairs and service, contact Technical Support. Do not perform
any repairs or service on the
e
Blot
TM
Protein Transfer Device by yourself to
avoid any possible damages to the device.
Disassembling and
Cleaning the
eBlot
TM
Device
Protocols,
continued
Performing Blotting,
continued
Protocols,
continued
Performing Blotting
2. Press the
Start/Stop
button to start the transfer. The running time begins to
count down and right Status Light keeps flashing during the whole transfer
program.
Perform protein blotting as described below within 15 minutes of assembling
the transfer stack.
1. Press the
Min.
and
Sec.
buttons to set appropriate running time based on
the protein size (see table below). If an undesired running time is set by
mistake, press
Reset
button to clear the wrong time, and then press the
Min.
and
Sec.
buttons to choose the desired running time.
Note: Based on the initial results, the transfer time may need to be optimized to make best
transfer results by pressing the Time button in 5-second increment. For proteins greater
than 200 kDa, we recommend to start with 11 minutes.
Protein size (kDa)
< 80
80-160
> 160
Recommended
start running time (min)
7
8-9
10
11. Press the
Open
button, and then push back and close the lid of
e
Blot
TM
Protein Transfer Device.
Assembling the Transfer
Stack,
continued
