Genevac HT-24 User Manual Download Page 24

 Genevac HT-24 

 

User Manual 

 
 

22

 

 

10-1562  Issue 3-7  March

 

2014 

Sample Volume 

Enter a value for the volume of sample in each individual well / tube / vial. 
 

 

 

 

 

 
Note:

  Once selections for 

Sample Holder Type

Sample Volume

 and 

Solvent

 have been 

made from the drop down lists, an option to 

Auto Program

 is offered.   

 
 
Select 

Y (Yes)

 for the evaporator software to auto programme the remaining run parameters 

Auto programmed run parameters are shown in blue but can be manually overwritten, in 
which case they revert to being shown in white.   
  
Alternatively, select 

N (No)

 to continue entering the remaining parameter fields manually.  

 
 

Note: 

 If the 

Auto Program

 function is used with more than one solvent selected, the 

evaporator sets run parameters for the higher boiling point solvent.  However, it is usually 
more efficient to set-up a run for the more volatile solvent and link this to another run (or 
runs) for the remaining component(s) of the mixture. 
 

 

Summary of Contents for HT-24

Page 1: ...Genevac HT 24 Evaporation System User Manual Original instructions Part number 10 1562 Issue 3 7 March 2014...

Page 2: ......

Page 3: ...ent container 32 Loading Samples 34 Sample holders 34 Use of correct accessories 34 Mass limit 35 Sample holders and swings 35 Loading the rotor 36 Balancing the rotor 37 Method guide 38 Pre programme...

Page 4: ...with the option of an integrated fume hood The workstation also accommodates two condensers a high vacuum scroll pump and a waste solvent container Scroll pump catch pot door open Workstation front vi...

Page 5: ...ing each evaporator independently to a PC Rear view with fume hood rear panel removed for clarity Two data connections waste solvent pump Three mains power supply connections electrical circuit breake...

Page 6: ...load samples select the appropriate run and press start Evaporator screen and keypad Evaporation progress is shown by a graphical display with plots for temperature and pressure The evaporator control...

Page 7: ...evaporation system Only operate the system in a ventilated space Make sure exhaust ventilation hoses are securely fitted and take solvent vapours away from the equipment and from the operator Only us...

Page 8: ...tion Only operate the system in a well ventilated environment and consider safety when evaporating any combustible solvents Genevac s position regarding the evaporation of such solvents particularly w...

Page 9: ...ar fumes before opening the fume hood doors In addition the following optional specifications can be added at the time of ordering Consult your Genevac representative for advice on any other requireme...

Page 10: ...shows just a few of the sample holders available Sample holders are manufactured in solid aluminium and are anodised to improve heat absorption The contact area is maximised to provide good physical s...

Page 11: ...o power the system down on a regular basis Note The workstation power switch can be left on at all times Power distribution to the entire system can be controlled the evaporator power switches To powe...

Page 12: ...perature The system will not allow an evaporation run to commence until the condenser reaches 0 C if an attempt is made to start the run will commence once operational temperature has been reached To...

Page 13: ...e evaporator doors before closing the fume hood doors Warning Risk of hand entrapment between the left hand evaporator and the side of the fume cupboard Take care when opening the evaporator door Stan...

Page 14: ...to open evaporator 2 right hand side evaporator 3 ON indicator Lights up to indicate workstation is powered 4 EMERGENCY STOP Press to isolate the workstation and stop both evaporators in an emergency...

Page 15: ...show different segments in time 5 SETTINGS Press to display the Run Data screen for the selected run 6 Keypad Enter data for setting up or editing runs 7 Evaporator power switch Controls distribution...

Page 16: ...y selected method number 2 Status shows current evaporator status 3 Graphical data Shows progression of evaporation run with data for Channel 1 temperature Channel 2 temperature Pressure and Spin Rate...

Page 17: ...SampleGuard channel 2 is optional SampleGuard probes are available in lengths to suit different types of sample holder Caution Risk to sample integrity make sure the probes are long enough to allow t...

Page 18: ...contact your local Genevac representative to request a Genevac Accessories Brochure or visit the Genevac website at www genevac com Selecting a run From the SELECT RUN screen use the cursor direction...

Page 19: ...races begin to converge this indicates the sample is approaching dryness Run parameters can be changed whilst the evaporator is operating refer to Advanced Operation Setting up a run Stopping a run To...

Page 20: ...ed Skip The evaporator returns to the Run Select screen Drain The system opens the drain valve and pumps any waste solvent into the waste solvent container Defrost Enter the defrost menu Select START...

Page 21: ...d The following section describes how to set up a new method the process is similar for overwriting an existing method Begin by selecting an empty slot from the SELECT RUN screen or select an existing...

Page 22: ...xt Run Number Runs can be linked together enter the number of the run that requires to be linked and it will commence when the current run finishes Any number of runs can be linked together in this wa...

Page 23: ...selected solvents are highlighted in blue Select Done when the solvent or solvents have been selected For solvents that are not listed select Other and enter the solvent name manually no more than 31...

Page 24: ...emaining run parameters Auto programmed run parameters are shown in blue but can be manually overwritten in which case they revert to being shown in white Alternatively select N No to continue enterin...

Page 25: ...SampleGuard Control Channel Channel 1 is the default setting The option to change to channel 2 is provided only to allow continued operation should a fault arise with channel 1 or its associated Samp...

Page 26: ...cuum chamber to cool before evaporating volatile solvents or mixtures that may be prone to bumping this is unnecessary for most applications function Automatic Control To optimise the cryo pumping eff...

Page 27: ...ly if Dri Pure is selected see Pressure Control Regime Low Samples are subject to lower centrifugal force approximately 350 G This should be used for applications that do not require high rotor speed...

Page 28: ...uired to maintain the sample holders at control temperature When sample dryness is detected the system automatically turns off the IR lamps and performs a final drying stage before stopping the run Sa...

Page 29: ...The evaporator selects high rotor speed and reduces the chamber pressure slowly The IR lamps do not switch on during the Dri Pure ramp Select this setting if bumping is anticipated Variable Dri Pure...

Page 30: ...ges This Stage time elapsed since start of current stage changes to End of Run or End of Stage and begins to count down when final run time is known Graphical display for Channel 1 Temperature Channel...

Page 31: ...nu screens Menu bar The default position for the horizontal menu bar cursor is with START highlighted Press the cursor direction left and right keys to return the cursor to START after using any of th...

Page 32: ...ta key evaporator methods will remain unchanged all other methods will be overwritten COPY ALL METHODS TO DATAKEY Methods can be copied for safekeeping or for cloning another system COPY LOG FILES TO...

Page 33: ...in one operation but using automatic defrost and drain methods can be set up to perform multiple operations without the need for manual intervention between stages Automatic defrost and drain also hel...

Page 34: ...e will continue until the system senses that all solvent in the condenser pot is liquid or can be stopped manually by pressing the STOP button To drain liquid solvents from the condenser without going...

Page 35: ...urn counter clockwise to release the drain pipe then pull the drain pipe out of the container Pull the breather pipe out of the container and disconnect the electrical earth strap from the container l...

Page 36: ...le contact your local Genevac representative and request a Genevac Accessories Brochure Use of correct accessories Take care to make sure that the correct sample holders are used follow this guidance...

Page 37: ...per swing This is the total mass that can be loaded onto each position of a swung rotor and includes The swing The sample holder The tubes or vials The sample solutions Do not exceed the mass limit un...

Page 38: ...tribute tubes symmetrically in sample holders Make sure tubes or vials are inserted fully into the sample holder wells If using separate swings make sure the sample holders are located correctly in th...

Page 39: ...tribute the samples into four holders to help distribute the heat flow evenly between samples and to reduce mechanical stress on the rotor Alternatively two empty swings can be placed on the unused ro...

Page 40: ...cetate or methanol Single volatile solvents with no risk of bumping 52 Very volatile DCM 40 to 90 C DCM methylene chloride or chloroform Volatile solvent mixtures and DCM Methylene chloride where risk...

Page 41: ...lyophilisation methods it will also indicate when the monitored sample has frozen and when the lyophilisation process ends Use the data to optimise the method for example if it can be proven that a f...

Page 42: ...yl chloride SOCl2 Dichloromethane DCM Toluene Diisopropyl ether DIPE Trichloroacetic acid TCA Dimethyl acetamide Triethylamine Et3N N N Dimethyl formamide DMF Trifluroacetic acid TFA Dimethyl sulfoxid...

Page 43: ...orate a mixture of DMSO and DCM Specific method settings would depend on variables such as volume mixture ratio and the type sample holder used 1 Select Variable Dri Pure and set a ramp from 750 mbar...

Page 44: ...Your Volume Your Volume Your Volume Solvent MeOH H20 MeOH H20 MeOH H20 SampleGuard Control Temp C 40 40 40 SampleGuard Control Channel 1 1 1 CoolHeat Enable Pressure mbar 50 50 50 Chamber Temperature...

Page 45: ...40 0 40 SampleGuard Control Channel 1 1 1 CoolHeat Enable Pressure mbar 50 50 50 Chamber Temperature control c preheat to 20 preheat to 0 preheat to 30 Rotor Speed High High Low Heat Off Option Elapse...

Page 46: ...r Volume Your Volume Solvent DMSO or DMF DCM DMSO or DMF DCM DMSO or DMF DCM SampleGuard Control Temp C 40 40 40 SampleGuard Control Channel 1 1 1 CoolHeat Enable Pressure mbar 50 50 50 Chamber Temper...

Page 47: ...e of solvent is evident on the inside of the evaporation chamber or if any solvent is spilled For further instructions refer to the following sections General cleaning and Sample holders Periodic main...

Page 48: ...as been in contact with biological agents a bleach solution may be used IR lamp lenses Using acetone applied to a paper towel or cloth remove all traces of solvent deposit from the IR lamp lenses Depe...

Page 49: ...ll not affect performance however if there is any structural damage if any part of the swing or sample holder is bent or deformed or if there is any sign of a crack do not use it Contact Genevac Servi...

Page 50: ...l position The back of the system may be positioned against a wall although provision must be made for maintenance access Leave sufficient space on either side of the workstation to allow the waste so...

Page 51: ...the unlikely event that an error occurs that that could affect the physical condition of the evaporator or its ability to safeguard the integrity of the samples a critical error the system will stop...

Page 52: ...ol Check the sample swings are able to move freely Clean the quartz glass using a suitable solvent such as methanol or acetone and a lint free cloth or paper towel Clean the swings and rotor in the sa...

Page 53: ...power In the event of power failure the vacuum system vents automatically the rotor may take up to two minutes to stop spinning Once the rotor is stationary and the vacuum system is at atmospheric pr...

Page 54: ...fault this is normal Wait a few minutes for the condenser to reach operating temperature Condenser not ready after 10 minutes Contact Genevac service Out of balance error Rotor imbalance detected at...

Page 55: ...er fails to drain Possible failure of condenser drain pump or blockage of drain pump filter Contact Genevac Service for assistance Samples not dry on lower rotor levels only Lamp layers disabled Go to...

Page 56: ...e Sea level to 12000 m Operating environment Ambient temperature 15 C to 30 C Relative humidity 10 to 60 non condensing Altitude Sea level to 1600 m Ingress protection rating IP30 Note This evaporator...

Page 57: ...0 Safety requirements for electrical equipment for measurement control and laboratory use General requirements BS EN 60204 1 2006 A1 2009 Safety of machinery Electrical equipment of machines General r...

Page 58: ...ailed in the Maintenance section of this User manual Power leads supplies with the system are for use with that system only If items are replaced by the owner only Genevac approved parts may be used I...

Page 59: ...release only update waste solvent container instructions for manual release connector add Troubleshooting note 01 May 12 3 4 Power specifications revised Screen shots updated to reflect software chan...

Page 60: ...oing to press and may be subject to change without notice Some of the features and software functions described within this user manual may not apply to equipment manufactured before this manual s pub...

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