29-0047-78 AC 04-2014
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All spectrophotometers are operated in the same generic way by comparing the
amount of light Absorbed by a sample when compared to a reference solution:
2.1. Preparation before starting
• The instrument is switched on and allowed to initialise, Ultrospec 10,
Novaspec III/Plus, GeneQuant 100/1300, SimpliNano and NanoVue Plus units have
the on/off switch on the keypad.
• Ultrospec 2100 units have an on/off switch on the back of the instrument on the
right hand side.
• Ultrospec 7000/8000/9000 instruments have the on/off switch on the left hand
side of the instrument.
• The relevant software module for the application being carried out is selected from
within the instrument software e.g. fixed wavelength measurement, concentration
measurement, wavelength scan, kinetics measurement or a dedicated method
such as DNA concentration & purity. The options within the application set up the
instrument parameters such as wavelength and define any calculations taking
place on the measured results.
2.2. Performing a run - cuvettes
• On the Ultrospec 10, Novaspec III/Plus, GeneQuant 100/1300 and Ultrospec
2100/7000/8000/9000 units, samples are measured in a cuvette. A suitable
cuvette must be used for the application to be carried out – it must be of the
correct material to allow the selected wavelength of light to pass through and
must be of the correct physical size to fit the relevant instrument and the sample
volume available. The cuvette is filled with a solution of reference material which
is typically the solution that the sample is dissolved in, the cuvette is placed in the
cell holder in the correct orientation as per the diagrams below:
Ultrospec 10, front to back All other units, right to left
• The instrument is then zeroed by pressing the Zero key on the keypad:
2. OPERATING AND
MAINTENANCE
Ultrospec 10 Novaspec III/Plus GeneQuant 100/1300 Ultrospec 2100
