GE TYPHOON FLA 7000 Getting Started Manual Download Page 91

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Reading phosphorimaging samples

In this chapter

This chapter contains the following sections:

See page

Section

7.1 Set the reading conditions

7.2 Place the storage phosphor screen on the phosphor stage

7.3 Place the phosphor stage in the Typhoon FLA 7000

7.4 Read the sample

Getting Started with Typhoon FLA 7000 28-9607-64 AD

7 Reading phosphorimaging samples

Summary of Contents for TYPHOON FLA 7000

Page 1: ...Getting Started with Typhoon FLA 7000 Original instructions ...

Page 2: ...A 7000 Control Software for Windows 7 58 3 5 4 Installing 64 bit Typhoon FLA 7000 control software for Windows 7 70 3 6 Preparations before starting Typhoon FLA 7000 71 4 Operation 72 4 1 Operation overview 73 4 2 Starting the instrument and the Typhoon FLA 7000 control software 76 5 Reading fluorescent samples 77 5 1 Set the reading mode and reading conditions 79 5 2 Place the sample on the stage...

Page 3: ...ace the stage in the Typhoon FLA 7000 103 8 4 Read the sample 104 Appendix A Other settings 106 Appendix B Daily maintenance 107 Appendix C Periodic maintenance 108 Appendix D Installing and replacing filters 113 Appendix E Default sample detection methods 115 Appendix F Specifications Getting Started with Typhoon FLA 7000 28 9607 64 AD 3 Table of Contents ...

Page 4: ... in a safe way Prerequisites In order to operate the Typhoon FLA 7000 in the way it is intended the following prereq uisites must be fulfilled You have read and understood the safety instructions outlined in this manual The instrument is installed according to the instructions in this manual In this chapter This chapter contains important user information and a general description of the Typhoon F...

Page 5: ...safety notices WARNING CAUTION and NOTICE concerning the safe use of the product See definitions below WARNING WARNING indicates a hazardous situation which if not avoided could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood CAUTION CAUTION indicates a hazardous situation which if not avoided could result in minor or mode...

Page 6: ... optimize your procedures Tip Typographical conventions Software items are identified in the text by bold italic text A colon separates menu levels thus File Open refers to the Open command in the File menu Hardware items are identified in the text by bold text for example Power 6 Getting Started with Typhoon FLA 7000 28 9607 64 AD 1 Introduction 1 1 Important user information ...

Page 7: ...nized standards Title Directive Machinery Directive MD 2006 42 EC Electromagnetic Compatibility EMC Directive 2004 108 EC Low Voltage Directive LVD 2006 95 EC International standards This product fulfills the requirements of the following standards Notes Description Standard EN 61010 1 harmo nized with EU directive 2006 95 EC Safetyrequirementsforelectri cal equipment for measure ment control and ...

Page 8: ...ed to other products recommended or described in the user documentation and used in the same state as it was delivered from GE except for alterations described in the user documentation FCC compliance This device complies with part 15 of the FCC Rules Operation is subject to the following two conditions 1 This device may not cause harmful interference and 2 this device must accept any interference...

Page 9: ... is likely to cause harmful interference in which case the user will be re quired to correct the interference at his own expense Environmental requirement Title Requirement Restriction of Hazardous Substances RoHS Directive 2011 65 EU Waste Electrical and Electronic Equipment WEEE Directive 2012 19 EU Administration on the Control of Pollution Caused by Elec tronic InformationProducts China Restri...

Page 10: ... CW 650 nm LD laser class 3B The beam divergence of all laser modules is collimated Note Regulatory compliance of connected equipment Any equipment connected to Typhoon FLA 7000 should meet the safety requirements of EN IEC 61010 1 or relevant harmonized standards Within EU connected equipment must be CE marked 10 Getting Started with Typhoon FLA 7000 28 9607 64 AD 1 Introduction 1 2 Regulatory in...

Page 11: ...phor screens Four lasers and multiple filters enable the use of a variety of fluorescent dyes and detection methods Data is aquired through high resolution sensitivity and wide range linearity Compatibility with various types of samples for example fluorescent labels fluores cent dyes CBB stain silver stain X ray film and storage phosphor screens Getting Started with Typhoon FLA 7000 28 9607 64 AD...

Page 12: ...F G H I J K L M N O P 1 2 3 4 5 6 7 8 9 Function Part Power switch located between the AC connector and the USB connector 1 Indicator lamps 2 Knob 3 Lid 4 Setting block 5 Filter change door 6 Filter module slot 7 Filter module 8 Filter 9 12 Getting Started with Typhoon FLA 7000 28 9607 64 AD 1 Introduction 1 3 The Typhoon FLA 7000 laser scanner ...

Page 13: ... Protection cover for storage phosphor screen 12 Fluor Stage 13 Digitizing fluorescent plate 14 Membrane weight included in Fluor stage 15 Getting Started with Typhoon FLA 7000 28 9607 64 AD 13 1 Introduction 1 3 The Typhoon FLA 7000 laser scanner ...

Page 14: ...a Business SP2 32 bit or Microsoft Windows 7 Professional 32 bit or Microsoft Windows 7 Professional 64 bit 1 GB Internal memory Intel Core 2 Duo processor Processor 80 GB Hard drive 1280 1024 pixels Monitor resolution One USB 2 0 port Other requirements DVD ROM drive 14 Getting Started with Typhoon FLA 7000 28 9607 64 AD 1 Introduction 1 4 Typhoon FLA 7000 control software ...

Page 15: ...k to perform digitization 3 Method button click to register or erase a combination of laser and filter 4 Filter Module button click to change or register a filter 5 Preferences button click to set the image file format scan mode correction mode and to enable or disable the ND filter 6 Filter displays the loaded filters 7 Laser displays the status of the loaded laser units 8 Getting Started with Ty...

Page 16: ...the file where the image is saved and can be viewed with the analyzing software 3 Method set the method to use in the scan Up to 4 scans can be performed in a row all with individual methods 4 PMT set the voltage of the photo multiplier tube The higher the value the higher the sensitivity 5 Click to increase the number of scans Click to decrease the number of scans 16 Getting Started with Typhoon ...

Page 17: ...the mode for the reading area borders 9 Select Grid to limit the borders to the grid lines Select Free for an arbitrary area Select All to use the entire Fluor stage as the reading area Save Condition click this button to save the current reading conditions in a file if desired For details refer to the User Manual 10 Load Condition click this button to load previously saved reading conditions if d...

Page 18: ... the following sections See page Section 19 2 1 Safety precautions 25 2 2 Labels 30 2 3 Emergency procedure 31 2 4 Recycling information 32 2 5 Declaration of Hazardous Substances DoHS 18 Getting Started with Typhoon FLA 7000 28 9607 64 AD 2 Safety instructions ...

Page 19: ...t in fire or electric shock Stop using the equipment immediately turn off the power switch and unplug the equipment from the power outlet Contact your local GE representative to request repair WARNING Do not damage the power supply cord by bending twisting heating or allowing them to become pinned under the equipment Using damaged power cords could result in fire or electric shock If the power sup...

Page 20: ...h as lenses filters or lights Laser safety WARNING Never detach the inner cover screwed to this instrument If it is detached laser beam may leak with a risk of loss of vision CAUTION Never cancel the interlocks in this instrument laser beam may leak with a risk of loss of vision CAUTION Using procedures or adjustments other than those specified in this manual may result in hazardous exposure to la...

Page 21: ...w the instructions of your radiation administra tor Personal protection CAUTION Always wear gloves protective glasses and a lab coat or similar when handling samples CAUTION Always wear cotton gloves when handling Storage phosphor screens CAUTION Wear gloves protective glasses and a lab coat or similar when disposing of the Typhoon FLA 7000 Installing and moving the instrument WARNING The Typhoon ...

Page 22: ...AUTION Do not connect any USB devices other than the Typhoon FLA 7000 to the computer in which the Typhoon FLA 7000 Control Software is installed Use only the Typhoon FLA 7000 Control Software during read ing System operation WARNING Do not use the equipment with a power supply other than that recommended Fire and electric shock could result WARNING Do not use the equipment within or near a sink o...

Page 23: ...age phosphor screens in places where the en vironmental radiation may be increased for example rooms with concrete walls or in basements Maintenance WARNING Do not attempt to modify the equipment or fire and electric shock could result WARNING Do not use excessive amounts of liquids for cleaning the Typhoon FLA 7000 this may result in product malfunction or electric shock CAUTION Wear gloves to pr...

Page 24: ...er wise the equipment may be influenced by electrical nosie CAUTION Turn off the power switch and remove connecting cables before moving the equipment CAUTION Turn the power switch off before cleaning the inside of the equip ment CAUTION Unplug the equipment if it will not be used for an extended period 24 Getting Started with Typhoon FLA 7000 28 9607 64 AD 2 Safety instructions 2 1 Safety precaut...

Page 25: ...he user documentation before using the system Do not open any covers or replace parts unless specifically stated in the user documentation The system complies with the requirements for electromagnetic compli ance EMC in Australia and New Zealand The system complies with applicable European directives Getting Started with Typhoon FLA 7000 28 9607 64 AD 25 2 Safety instructions 2 2 Labels ...

Page 26: ...rd SJ T11363 2006 Requirements for Concentration Limits for Certain Hazardous Substances in Electronics Labels concerning laser light Meaning Label CAUTION Avoid exposure to the laser beam when the lid is open CAUTION Class 3B Laser product when open and interlock defeated Avoid expo sure to the beam Do not attempt to defeat the safety inter locks under the sample lid or behind the filter door or ...

Page 27: ...Labels at the front of Typhoon FLA 7000 Getting Started with Typhoon FLA 7000 28 9607 64 AD 27 2 Safety instructions 2 2 Labels ...

Page 28: ...Labels at rear of Typhoon FLA 7000 28 Getting Started with Typhoon FLA 7000 28 9607 64 AD 2 Safety instructions 2 2 Labels ...

Page 29: ...Labels on left side of Typhoon FLA 7000 I Power ON O Power OFF USB Getting Started with Typhoon FLA 7000 28 9607 64 AD 29 2 Safety instructions 2 2 Labels ...

Page 30: ... from the power outlet 3 Power failure The results of a power failure depends on the unit or units affected Results Unit affected by power fail ure The reading is interrupted immediately The in strument is in an undefined state The data collected up to the time of the power failure is available in the file created when starting the scan Typhoon FLA 7000 instrument The computer shuts down immediate...

Page 31: ...f the User Manual If the pollution level exceeds the limit dispose of the instrument body as radioactive waste Otherwise dispose of the materials according to applicable laws and regulations for disposal of industrial waste Disposal of electrical components Waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact ...

Page 32: ...riod EFUP which indicates the period during which the toxic or hazardous substances or elements contained in electronic information products will not leak or mutate under normal operating con ditions so that the use of such electronic information products will not result in any severe environmental pollution any bodily injury or damage to any assets The unit of the period is Year In order to maint...

Page 33: ...所列数据为发布时所能获得的最佳信息 0 Indicates that this toxic or hazardous substance contained in all of the homogeneous ma terials for this part is below the limit requirement in SJ T11363 2006 X Indicates that this toxic or hazardous substance contained in at least one of the homoge neous materials used for this part is above the limit requirement in SJ T11363 2006 Data listed in the table represents best infor...

Page 34: ... See page Section 35 3 1 Site requirements 36 3 2 Transporting Typhoon FLA 7000 37 3 3 Delivery inspection 38 3 4 Connections 39 3 5 Software installation 70 3 6 Preparations before starting Typhoon FLA 7000 34 Getting Started with Typhoon FLA 7000 28 9607 64 AD 3 Installation ...

Page 35: ...A Power supply 50 to 60 Hz Line frequency Stable horizontal surface Placement Do not place in direct sunlight or in brightly lit places 10 C to 30 C Ambient temperature 20 to 75 non condensing Humidity 2000 m above sea level Maximum altitude Getting Started with Typhoon FLA 7000 28 9607 64 AD 35 3 Installation 3 1 Site requirements ...

Page 36: ...The Typhoon FLA 7000 weighs approximately 62 kg and requires at least two persons to lift and move Precautions before moving the instrument Action Step Turn off the Typhoon FLA 7000 1 Turn off the computer and any peripheral devices 2 Disconnect the power cords and the USB connection 3 36 Getting Started with Typhoon FLA 7000 28 9607 64 AD 3 Installation 3 2 Transporting Typhoon FLA 7000 ...

Page 37: ...g Typhoon FLA 7000 inspect the package for external damages Should you find any external damages notify the delivery company and contact GE for further advice Getting Started with Typhoon FLA 7000 28 9607 64 AD 37 3 Installation 3 3 Delivery inspection ...

Page 38: ...e USB cable to a USB port on the computer 2 CAUTION Do not connect any USB devices other than the Typhoon FLA 7000 to the computer in which the Typhoon FLA 7000 Control Software is installed Use only the Typhoon FLA 7000 Control Software during read ing Electrical power Connect the power cord of the Typhoon FLA 7000 to a grounded power outlet WARNING Use only power cords delivered or approved by G...

Page 39: ...ion is performed in the following sequence 1 Install the USB control driver 2 Install the USB function driver Windows XP only 3 Install theTyphoon FLA 7000 control software Getting Started with Typhoon FLA 7000 28 9607 64 AD 39 3 Installation 3 5 Software installation ...

Page 40: ...e wizard 3 Click the Next button in Add hardware wizard 4 Select Yes I have already connected the hardware and click Next 5 Select Add a new hardware device and click the Next button 6 Select Install the hardware that I manually select from a list Advanced and click the Next button 7 Select Show All Devices and click the Next button 8 Click the Have Disk button in the Add hardware wizard 9 40 Gett...

Page 41: ...d click the Open button 13 Click the OK button in the Install from disk dialog 14 Click the Next button in the Add hardware wizard 15 Click the Next button again 16 Click the Continue Anyway button in the Hardware Installation dialog 17 Getting Started with Typhoon FLA 7000 28 9607 64 AD 41 3 Installation 3 5 Software installation 3 5 1 Installing Typhoon FLA 7000 Control Software for Windows XP ...

Page 42: ...nd New Hardware Wizard dialog 3 Insert the installation DVD 4 Select Install the software automatically Recommended 5 Click the Next button in the Found New Hardware Wizard dialog 6 Click the Finish button to complete the installation 7 Install Typhoon FLA 7000 for Windows XP Action Step Insert the Typhoon FLA 7000 DVD 1 Locate and double click the file Typhoon FLA 7000 exe 2 In the Typhoon FLA 70...

Page 43: ...nation folder in the dialog 5 Click the Next button to install the software at the default folder C Pro gram Files Click the Change button to install to a different folder Click the Install button in the installation dialog 6 Click the Finish button to finish the installation of Typhoon FLA 7000 7 Getting Started with Typhoon FLA 7000 28 9607 64 AD 43 3 Installation 3 5 Software installation 3 5 1...

Page 44: ...Note Action Step Disconnect Typhoon FLA 7000 from the computer 1 Open the control panel and click Classic View in the upper left corner 2 Open Add Hardware 3 In the Add Hardware dialog click the Next button 4 Select Install the hardware that I manually select from a list Advanced and click the Next button 5 Select Show All Devices and click the Next button 6 Click the Have Disk button 7 Insert the...

Page 45: ...2 Click the Next button in the wizard Add hardware 13 Click the Next button once again 14 The following warning is displayed Proceed by clicking Install this driver software anyway 15 Click the Finish button in the Add Hardware wizard to complete the instal lation 16 Getting Started with Typhoon FLA 7000 28 9607 64 AD 45 3 Installation 3 5 Software installation 3 5 2 Installing Typhoon FLA 7000 Co...

Page 46: ... is not acceptable please contact a GE representative See the back cover of this manual for contact information 4 Select I accept the terms in the license agreement and click the Next button Select destination folder in the dialog 5 Click the Next button to install the software at the default folder C Pro gram Files Click the Change button to install to a different folder Click the Install button ...

Page 47: ...nish button 9 The installation of Typhoon FLA 7000 is now completed Connect the computer and the Typhoon FLA 7000 with the USB cable 10 Getting Started with Typhoon FLA 7000 28 9607 64 AD 47 3 Installation 3 5 Software installation 3 5 2 Installing Typhoon FLA 7000 Control Software for Windows Vista ...

Page 48: ...ction Step Click Start at the bottom left corner on the screen 1 Click Control Panel System and Security then click Change when the computer sleeps under Power Options 2 In the Put the computer to sleep list click Never 3 Click Save changes 4 48 Getting Started with Typhoon FLA 7000 28 9607 64 AD 3 Installation 3 5 Software installation 3 5 3 Installing 32 bit Typhoon FLA 7000 Control Software for...

Page 49: ...Start at the bottom left corner on the screen 1 Click Control Panel System and Security System to bring up View basic information about your computer window 2 Note the system type displayed in the window Use the information to choose the appropriate installer 3 Getting Started with Typhoon FLA 7000 28 9607 64 AD 49 3 Installation 3 5 Software installation 3 5 3 Installing 32 bit Typhoon FLA 7000 C...

Page 50: ...w If the following message is displayed Correction Cause Restart the installation using the installer for Windows 7 with 64 bit operating system The computer is running on Windows 7 with 64 bit operating system You have chosen the installer for Windows 7 with 32 bit oper ating system If the following message is displayed 50 Getting Started with Typhoon FLA 7000 28 9607 64 AD 3 Installation 3 5 Sof...

Page 51: ...to confirm your actions in a dialog with the text Windows needs your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation Note Action Step Disconnect Typhoon FLA 7000 from the computer 1 Open the control panel and click Hardware and Sound 2 Click Device Manager under Devices and Printers 3 Getting Started with Typhoon FLA 7000 28 9...

Page 52: ...rdware dialog click Next 5 Select Install the hardware that I manually select from a list Advanced and click Next 6 52 Getting Started with Typhoon FLA 7000 28 9607 64 AD 3 Installation 3 5 Software installation 3 5 3 Installing 32 bit Typhoon FLA 7000 Control Software for Windows 7 ...

Page 53: ...DVD and click Browse 9 Select to install the driver from the Typhoon FLA 7000 DVD 10 Select the USB Control folder and click Open 11 Getting Started with Typhoon FLA 7000 28 9607 64 AD 53 3 Installation 3 5 Software installation 3 5 3 Installing 32 bit Typhoon FLA 7000 Control Software for Windows 7 ...

Page 54: ...Add Hardware wizard 14 Click the Next button once again 15 The following warning is displayed Proceed by clicking Install this driver software anyway 16 54 Getting Started with Typhoon FLA 7000 28 9607 64 AD 3 Installation 3 5 Software installation 3 5 3 Installing 32 bit Typhoon FLA 7000 Control Software for Windows 7 ...

Page 55: ...ission to continue Enter an administrator password if prompted then click Continue to proceed with the installation Note Action Step Insert the Typhoon FLA 7000 DVD 1 Locate and double click the file Typhoon FLA 7000 exe 2 In the Typhoon FLA 7000 InstallShield Wizard dialog click the Next button 3 Getting Started with Typhoon FLA 7000 28 9607 64 AD 55 3 Installation 3 5 Software installation 3 5 3...

Page 56: ...reement and click the Next button Select the destination folder in the dialog 5 Click the Next button to install the software at the default folder C Pro gram Files Click the Change button to install to a different folder Click the Install button 6 56 Getting Started with Typhoon FLA 7000 28 9607 64 AD 3 Installation 3 5 Software installation 3 5 3 Installing 32 bit Typhoon FLA 7000 Control Softwa...

Page 57: ...lick Install this driver software anyway in the Windows Security dialog 8 Click the Finish button to complete the installation 9 Connect the computer and the Typhoon FLA 7000 with the USB cable 10 Getting Started with Typhoon FLA 7000 28 9607 64 AD 57 3 Installation 3 5 Software installation 3 5 3 Installing 32 bit Typhoon FLA 7000 Control Software for Windows 7 ...

Page 58: ...s described below Action Step Click Control Panel System and Security then click Change when the computer sleeps under Power Options 1 Select Never in the drop down menu by Put the computer to sleep then click Save changes 2 58 Getting Started with Typhoon FLA 7000 28 9607 64 AD 3 Installation 3 5 Software installation 3 5 4 Installing 64 bit Typhoon FLA 7000 control software for Windows 7 ...

Page 59: ...the system type displayed in the window Use the information to choose the appropriate installer 3 Choose the appropriate installer to prevent failure If the installer does not match with the type of the computer operating system the Typhoon FLA 7000 is not installed successfully If one of the following messages is dis played during the installation process follow the instructions below If the foll...

Page 60: ...ows 7 with 32 bit operating system You have chosen the installer for Windows 7 with 64 bit oper ating system Install USB control driver During software installation you may be asked to confirm your actions in a dialog with the text Windows needs your permission to continue Type an administrator password when prompted then click Continue Note Follow the instructions below to install the USB control...

Page 61: ...der Devices and Printers 3 On the Action menu click Add legacy hardware 4 In the Add Hardware dialog click Next 5 Getting Started with Typhoon FLA 7000 28 9607 64 AD 61 3 Installation 3 5 Software installation 3 5 4 Installing 64 bit Typhoon FLA 7000 control software for Windows 7 ...

Page 62: ...from a list Advanced and click Next 6 Select Show All Devices and click Next 7 Click Have Disk 8 62 Getting Started with Typhoon FLA 7000 28 9607 64 AD 3 Installation 3 5 Software installation 3 5 4 Installing 64 bit Typhoon FLA 7000 control software for Windows 7 ...

Page 63: ...ware DVD 2 Click Open 3 10 2 1 3 In the Installer for 64bit USB Control 64 folder click Open 11 Select DevMng64 inf and click Open 12 In Install from disk dialog click OK 13 Getting Started with Typhoon FLA 7000 28 9607 64 AD 63 3 Installation 3 5 Software installation 3 5 4 Installing 64 bit Typhoon FLA 7000 control software for Windows 7 ...

Page 64: ...r hardware is dis played click Next 15 When the message Would you like to install this device software is dis played click Install 16 64 Getting Started with Typhoon FLA 7000 28 9607 64 AD 3 Installation 3 5 Software installation 3 5 4 Installing 64 bit Typhoon FLA 7000 control software for Windows 7 ...

Page 65: ...an administrator password when prompted then click Continue Note Action Step Insert the Typhoon FLA 7000 DVD 1 In the Installer for 64 bit folder locate and double click the file Typhoon FLA 7000 for 64bit msi 2 In the Typhoon FLA 7000 InstallShield Wizard dialog click Next 3 Getting Started with Typhoon FLA 7000 28 9607 64 AD 65 3 Installation 3 5 Software installation 3 5 4 Installing 64 bit Typ...

Page 66: ... li cence agreement and click Next 5 Define the software destination folder in the Destination Folder dialog as described below 6 If the default folder C Program Files x86 is acceptable click Next If you want to use a different folder click Change and define a suitable destination folder Click Next 66 Getting Started with Typhoon FLA 7000 28 9607 64 AD 3 Installation 3 5 Software installation 3 5 ...

Page 67: ...programs wants access to your computer Click Allow 8 When the message Would you like to install this device software is dis played click Install 9 Getting Started with Typhoon FLA 7000 28 9607 64 AD 67 3 Installation 3 5 Software installation 3 5 4 Installing 64 bit Typhoon FLA 7000 control software for Windows 7 ...

Page 68: ...Typhoon FLA 7000 Action Step Click Start at the bottom left corner on the screen 1 Click Control Panel Programs then click Uninstall a program under Pro grams and Features 2 68 Getting Started with Typhoon FLA 7000 28 9607 64 AD 3 Installation 3 5 Software installation 3 5 4 Installing 64 bit Typhoon FLA 7000 control software for Windows 7 ...

Page 69: ... software Follow the instructions below to upgrade the Typhoon FLA 7000 Action Step Uninstall the current version of the Typhoon FLA 7000 as described in the section Uninstall the software on page 68 above 1 Install the new version of the Typhoon FLA 7000 as described above 2 Getting Started with Typhoon FLA 7000 28 9607 64 AD 69 3 Installation 3 5 Software installation 3 5 4 Installing 64 bit Typ...

Page 70: ...puter with a USB cable The air intake fan on the rear side of Typhoon FLA 7000 is unobstructed The air exhaust fan on the rear side of Typhoon FLA 7000 is unobstructed Typhoon FLA 7000 is not placed in direct sunlight or in a brightly lit place There are no objects on top of Typhoon FLA 7000 There is no stage in the stage rack 70 Getting Started with Typhoon FLA 7000 28 9607 64 AD 3 Installation 3...

Page 71: ... This chapter contains the following sections See page Section 72 4 1 Operation overview 73 4 2 Starting the instrument and the Typhoon FLA 7000 control soft ware Getting Started with Typhoon FLA 7000 28 9607 64 AD 71 4 Operation ...

Page 72: ...rs Turn on the Typhoon FLA 7000 Start the computer and launch the Typhoon FLA 7000 Control Software Set the reading parameters Place the stage and the sample in the Typhoon FLA 7000 Read the sample Phosphorimaging samples Expose the storage phosphor screen 72 Getting Started with Typhoon FLA 7000 28 9607 64 AD 4 Operation 4 1 Operation overview ...

Page 73: ...hoon FLA 7000 Action Step Push the power switch on the left side of the body to the I position 1 When the power switch is turned on the indicator lamps change as shown below 2 After turning on the power swich During self diagnosis or startup adjustment Ready for operation Getting Started with Typhoon FLA 7000 28 9607 64 AD 73 4 Operation 4 2 Starting the instrument and the Typhoon FLA 7000 control...

Page 74: ...y deteriorate The scanned data can then not be guaranteed Action Step Turn on the computer 1 Make sure that Typhoon FLA 7000 has completed the warm up after which only the power lamp on the upper left panel on the front of the Typhoon FLA 7000 is lit 2 Start Typhoon FLA 7000 control software from the Start menu or use the desktop shortcut 3 74 Getting Started with Typhoon FLA 7000 28 9607 64 AD 4 ...

Page 75: ...tus messages are as follows Explanation Message Cannot recognize Typhoon FLA 7000 Please check connection and power Disconnected Typhoon FLA 7000 is in self diagnosis Please wait Warm up The unit is ready to use Ready Getting Started with Typhoon FLA 7000 28 9607 64 AD 75 4 Operation 4 2 Starting the instrument and the Typhoon FLA 7000 control software ...

Page 76: ...following sections See page Section 77 5 1 Set the reading mode and reading conditions 79 5 2 Place the sample on the stage 82 5 3 Place the stage in Typhoon FLA 7000 83 5 4 Read the sample 76 Getting Started with Typhoon FLA 7000 28 9607 64 AD 5 Reading fluorescent samples ...

Page 77: ...r the fluorescence mode Set the reading conditions by following the steps in the table below All parts in the window are described in Overview of the main window on page 15 Note 1 2 3 4 7 6 10 11 8 5 9 Description Part Image folder specify where to save the file after the reading 1 File Name enter the name of a file to save image data 2 Getting Started with Typhoon FLA 7000 28 9607 64 AD 77 5 Read...

Page 78: ...all pixel size for high quality images Note that a small pixel size setting increases the reading time and the size of the image file Latitude Specify the dynamic range 8 L5 provides a larger detectable range than L4 L4 provides a finer density gradation if the signals of the sample areas are in the correct range Mode Specify the mode for the reading area borders 9 Select Grid to limit the borders...

Page 79: ...sample on the Fluor stage Note The sample must not be thicker than 30 mm For solutions the maximum height is 4 mm Make sure there are no bubbles or gaps on the contact surface Action Step Place the sample on the Fluor stage 1 Getting Started with Typhoon FLA 7000 28 9607 64 AD 79 5 Reading fluorescent samples 5 2 Place the sample on the stage ...

Page 80: ...lace the titer plate in the desired position in the titer plate frame 2 Placing a gel sample with glass on the Multi stage Action Step Place a gel sample with glass on the Multi stage 1 80 Getting Started with Typhoon FLA 7000 28 9607 64 AD 5 Reading fluorescent samples 5 2 Place the sample on the stage ...

Page 81: ...d down the spring loaded glass holders on the glass 2 Tighten the screws on the glass holders as necessary 3 Getting Started with Typhoon FLA 7000 28 9607 64 AD 81 5 Reading fluorescent samples 5 2 Place the sample on the stage ...

Page 82: ...the lid 1 Position the stage so that the black triangle mark is aligned with the corre sponding mark on the setting block Fit the setting block pin in the hole of the stage 2 Pull the knob up and close the lid 3 82 Getting Started with Typhoon FLA 7000 28 9607 64 AD 5 Reading fluorescent samples 5 3 Place the stage in Typhoon FLA 7000 ...

Page 83: ...m the location where the reading was stopped Note Adjust the display parameters Explanation Parameter Adjust the gradations in the image Curve Exponential gradation adjustment Exponential Linear gradation adjustment Linear Sigmoid gradation adjustment Sigmoid Zoom in or out Magnification Check this option to optimize the tones automatically Auto range scope Getting Started with Typhoon FLA 7000 28...

Page 84: ...pter contains the following sections See page Section 85 6 1 Handling precautions 87 6 2 Preparing the Storage phosphor screen 89 6 3 Exposing the Storage phosphor screen 84 Getting Started with Typhoon FLA 7000 28 9607 64 AD 6 Exposing Storage phosphor screens ...

Page 85: ... phosphor screen CAUTION Always wear cotton gloves when handling Storage phosphor screens NOTICE Use a suction rod when taking the Storage phosphor screen out of the cassette Prying the Storage phosphor out may result in the edge peeling off making the Storage phosphor screen unusable NOTICE Do not scratch or bend the Storage phosphor screen Keep the Storage phosphor screen free from dust Getting ...

Page 86: ...f the Storage phosphor screen is reduced Volatile solvents may deform the protective film on the Storage phosphor screen NOTICE Protect exposed Storage phosphor screens from light until the reading is finished Storage NOTICE Remove the Storage phosphor screen from the Typhoon FLA 7000 main unit when not in use Store the Storage phosphor screens in a horizontal position protected from moisture 86 G...

Page 87: ...se the Storage phosphor screen The FLA Image Eraser can erase the Storage phosphor screen in around 15 minutes if it is not overly exposed For details refer to the FLA Image Eraser User Manual Dim the lighting Dim the ambient light to 20 lux or less before opening or moving the Storage phosphor screen without a cover Protect the phosphor stage with the protective cover when removing an exposed Sto...

Page 88: ...ieth of the time required for X ray film Take this into consideration when setting the initial exposure time Note For increased image quality minimize the time between exposing the Storage phosphor screen and reading it with the Typhoon FLA 7000 Tip 88 Getting Started with Typhoon FLA 7000 28 9607 64 AD 6 Exposing Storage phosphor screens 6 2 Preparing the Storage phosphor screen ...

Page 89: ...or screen A tritium storage phosphor screen can be used only once 2 Open the top cover of the cassette Unlock the cassette by sliding the buttons on both sides up 3 Place the sample on the cassette Make sure that the sample surface faces up 4 Note Keep the radio imaging sample away from the edge of the Storage phosphor screen Otherwise the recorded data may become corrupted Getting Started with Ty...

Page 90: ...mple Make sure that the notch of the Storage phosphor screen is in the front left corner of the cassette as shown below 5 Close the cover of the cassette Press on the cover until it clicks into place 6 90 Getting Started with Typhoon FLA 7000 28 9607 64 AD 6 Exposing Storage phosphor screens 6 3 Exposing the Storage phosphor screen ...

Page 91: ...ections See page Section 92 7 1 Set the reading conditions 95 7 2 Place the storage phosphor screen on the phosphor stage 96 7 3 Place the phosphor stage in the Typhoon FLA 7000 97 7 4 Read the sample Getting Started with Typhoon FLA 7000 28 9607 64 AD 91 7 Reading phosphorimaging samples ...

Page 92: ...horimaging mode Set the reading conditions by following the steps in the table below All parts in the window are described in Overview of the main window on page 15 Note 1 2 3 4 5 6 7 8 9 10 11 Description Part Image folder specify where to save the file after the reading 1 File Name enter the name of a file to save image data 2 92 Getting Started with Typhoon FLA 7000 28 9607 64 AD 7 Reading phos...

Page 93: ...ing area 2 Drag the sides of the red square as needed to adjust the size of the scanning area Pixel Size set the pixel size 7 Choose a small pixel size for high quality images Note that a small pixel size setting increases the reading time and the size of the image file Latitude Specify the dynamic range 8 L5 provides a larger detectable range than L4 L4 provides a finer density gradation if the s...

Page 94: ... click this button to load previously saved reading conditions if desired For details refer to the User Manual 11 94 Getting Started with Typhoon FLA 7000 28 9607 64 AD 7 Reading phosphorimaging samples 7 1 Set the reading conditions ...

Page 95: ...e phosphor stage 2 A B C D E F G H I J K L M N O P A B C D E F G H I J K L M N O P Pick up the storage phosphor screen and move it to the phosphor stage 3 Place the storage phosphor screen on the back of the phosphor stage with the white or blue reading surface of the storage phosphor screen facing up 4 Getting Started with Typhoon FLA 7000 28 9607 64 AD 95 7 Reading phosphorimaging samples 7 2 Pl...

Page 96: ... that the black triangle mark is aligned with the corre sponding mark on the setting block 2 Fit the setting block pin in the hole of the stage 3 Pull the knob up and close the lid 4 96 Getting Started with Typhoon FLA 7000 28 9607 64 AD 7 Reading phosphorimaging samples 7 3 Place the phosphor stage in the Typhoon FLA 7000 ...

Page 97: ...annot be resurmed from the location where the reading was stopped Note Adjust the display parameters Explanation Parameter Adjust the gradations in the image Curve Exponential gradation adjustment Exponential Linear gradation adjustment Linear Sigmoid gradation adjustment Sigmoid Zoom in or out Magnification Check this option to optimize the tones automatically Auto range scope Getting Started wit...

Page 98: ...owing sections See page Section 99 8 1 Set the reading mode and reading conditions 101 8 2 Place the sample on the stage 102 8 3 Place the stage in the Typhoon FLA 7000 103 8 4 Read the sample 98 Getting Started with Typhoon FLA 7000 28 9607 64 AD 8 Reading digitization samples ...

Page 99: ... the digitization mode Set the reading conditions by following the steps in the table below All parts in the window are described in Overview of the main window on page 15 Note 1 2 3 4 5 6 7 8 9 10 11 Description Part Image folder specify where to save the file after the reading 1 File Name enter the name of a file to save image data 2 Getting Started with Typhoon FLA 7000 28 9607 64 AD 99 8 Readi...

Page 100: ...ng increases the reading time and the size of the image file Latitude Specify the dynamic range 8 L5 provides a larger detectable range than L4 L4 provides a finer density gradation if the signals of the sample areas are in the correct range Mode Specify the mode for the reading area borders 9 Select Grid to limit the borders to the grid lines Select Free for an arbitrary area Select All to use th...

Page 101: ...ions the maximum height is 4 mm Make sure there are no bubbles or gaps on the contact surface Action Step Place the sample on the Fluor stage 1 Place the fluorescent plate for digitization on top of the sample 2 Getting Started with Typhoon FLA 7000 28 9607 64 AD 101 8 Reading digitization samples 8 2 Place the sample on the stage ...

Page 102: ... with the corre sponding mark on the setting block Fit the setting block pin in the hole of the stage 2 Gently place the fluorescent plate for digitizing on top of the digitized sample 3 Pull the knob up and close the lid 4 102 Getting Started with Typhoon FLA 7000 28 9607 64 AD 8 Reading digitization samples 8 3 Place the stage in the Typhoon FLA 7000 ...

Page 103: ...cannot be resurmed from the location where the reading was stopped Note Adjust the display parameters Explanation Parameter Adjust the gradations in the image Curve Exponential gradation adjustment Exponential Linear gradation adjustment Linear Sigmoid gradation adjustment Sigmoid Zoom in or out Magnification Check this option to optimize the tones automatically Auto range scope Getting Started wi...

Page 104: ...with each laser Correc tion Mode Manual The correction method must be selected individually in the Reader Settings window Note The correction mode settings list is registered by a serviceman Please contact the dealer where you purchased Typhoon FLA 7000 or contact GE Enables the use of an ND filter to adjust the light intensity Use the ND filter if the signal from the sample is very strong ND Filt...

Page 105: ...ith a gel file a read image can also be saved in TIFF file format For TIFF files im age data type is always set to Linear format Contains the path to the application used to analyze the data Launch Applica tion Select the analysis application by clicking the Select button Then browse to the software executable and click OK User account specific settings Changes to the settings in the Preferences d...

Page 106: ...ove the stage from the main body of the instrument 1 Wipe the stage with a sponge moistened with a fluorescence free neutral detergent 2 Thoroughly rinse the stage with water and dry with a lint free cloth 3 Place the stage in the main body of the instrument 4 106 Getting Started with Typhoon FLA 7000 28 9607 64 AD B Daily maintenance ...

Page 107: ... least once every 30 days to exe cute calibration This calibration makes sure that the SHG laser operates properly Failure to calibrate the Typhoon FLA 7000 every 30 days reduces the lifespan of the SHG laser Operation procedures 1 Turn on the Typhoon FLA 7000 and the computer 2 Wait until warm up and self diagnosis is completed and the scanner is ready Only the power lamp on the upper left panel ...

Page 108: ...from the filter module 3 Install the new filter in the filter module 4 Place the filter module in the scanner 5 Register the filter change in the Typhoon FLA 7000 Remove the filter module from the scanner Action Step Click the Filter Module button in the main window 1 The following status message appears Wait until the message is closed before proceeding to the next step 108 Getting Started with T...

Page 109: ...er module straight out 3 Remove and install filters in the filter module Action Step Press the notch on the bottom of the filter module and pull out the filter 1 Getting Started with Typhoon FLA 7000 28 9607 64 AD 109 D Installing and replacing filters ...

Page 110: ...the filter module 2 Push the new filter in until it locks in place Place the filter module in the scanner Action Step Open the filter change door 1 110 Getting Started with Typhoon FLA 7000 28 9607 64 AD D Installing and replacing filters ...

Page 111: ... filter change door 3 Register the filter change in Typhoon FLA 7000 Action Step Click the Filter Module button in the main window 1 Click the desired filter position on the filter tray 2 Getting Started with Typhoon FLA 7000 28 9607 64 AD 111 D Installing and replacing filters ...

Page 112: ...List 3 Click the Insert button to register the filter 4 Tip You can also click and drag the filter from Filter List to the desired filter posi tion Click the OK button 5 112 Getting Started with Typhoon FLA 7000 28 9607 64 AD D Installing and replacing filters ...

Page 113: ...O580 SYPRO Ruby Y520 SYPRO Orange Y520 FAM Y520 Alexa Fluor 488 Y520 Digitize473 532 nm laser Filter type Method name O580 Cy3 O580 EtBR O580 ROX O580 Pro Q Diamond O580 Digitize532 635 nm laser Filter type Method name R670 Cy5 Getting Started with Typhoon FLA 7000 28 9607 64 AD 113 E Default sample detection methods ...

Page 114: ...er type Method name R670 Alexa Fluor 633 R670 DDAO 650 nm laser Filter type Method name IP Phosphorimaging R710 CBB 114 Getting Started with Typhoon FLA 7000 28 9607 64 AD E Default sample detection methods ...

Page 115: ... image size Reading mode 20 cm x 40 cm maximum Storage phosphor screen 18 cm x 36 cm valid field angle 24 cm x 40 cm maximum Fluorescent 22 cm x 36 cm valid field angle 238 mm x 398 mm setting size 24 cm x 40 cm maximum Digitization 22 cm x 36 cm valid field angle 238 mm x 398 mm setting size Data Parameter 25 50 100 or 200 µm selectable Pixel size Getting Started with Typhoon FLA 7000 28 9607 64 ...

Page 116: ... x 427 5 x 15 Storage phosphor screen cassette Approx 2 429 x 283 x 35 Phosphor stage Approx 1 429 x 283 x 35 Fluor stage Approx 1 3 429 x 283 x 33 Multi stage 14 5 603 x 512 x 163 FLA Image Eraser The Reading block is a standard unit The other units are optional Note Power supply Data Parameter 100 to 240 V AC Input voltage 10 Allowable variations in voltage 50 to 60 Hz Frequency 1 5 to 3 0 A Rat...

Page 117: ... lighting level to about 20 lux when moving a sample from the cassette onto the Phosphor stage after exposure 20 lux is the brightness level at which most people can read newspaper sized text after he she becomes accustomed to the brightness Lighting Indoor use only Location of use 2 000 m above sea level Maximum altitude for use Transient overvoltage category II Overvoltage category Pollution Deg...

Page 118: ...rvices are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare repre sentative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshir...

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