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GE MicroCal iTC200, Operating Instructions Manual

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GE MicroCal iTC200 Operating Instructions Manual Download Page 6

1  Introduction

1.1   Important user information

6

MicroCal iTC

200

 

Operating Instructions

 

28-9639-77

 

AA

1.1

Important user information

Read this before using the 
MicroCal iTC

200

All users must read the Safety Instructions in 

Chapter 2

 of these Operating Instructions 

before installing, using or maintaining the system.

Do not operate the MicroCal iTC

200

 in any other way than described in the user 

documentation. If you do, you may be exposed to hazards that can lead to personal 
injury and you may cause damage to the equipment.

Intended use

The MicroCal iTC

200

 is an Isothermal Titration Calorimeter system designed for bio-

molecular interaction studies in research applications.

The MicroCal iTC

200

 system is intended for research use only and shall not be used in any 

clinical procedures or for diagnostic purposes.

Safety notices

These Operating Instructions contain WARNINGS, CAUTIONS and NOTICES concerning 
the use of the product, with meanings as defined below.

WARNING

WARNING indicates a hazardous situation which, if not avoided, could 
result in death or serious injury. It is important not to proceed until all 
stated conditions are met and clearly understood.

CAUTION

CAUTION indicates a hazardous situation which, if not avoided, could 
result in minor or moderate injury. It is important not to proceed until all 
stated conditions are met and clearly understood.

NOTICE

NOTICE indicates instructions that must be followed to avoid damage to 
the product or other equipment.

Summary of Contents for MicroCal iTC200

Page 1: ...GE Healthcare MicroCal iTC200 System Operating Instructions Original Instructions ...

Page 2: ......

Page 3: ...3 6 Configuring a MicroCal iTC200 controller for networking 38 4 Operation 39 4 1 Procedure before a run 39 4 2 Basics of performing a run 39 4 3 Loading the syringe 39 4 4 Loading the cell 40 4 5 Experimental design 42 4 6 Advanced experimental design 43 4 7 Instrument controls 44 4 8 Real time plot 46 4 9 Set up 46 4 10 Procedures after a run 47 5 Maintenance 49 5 1 Cell cleaning 49 5 2 Removing...

Page 4: ...able of Contents 4 MicroCal iTC200 Operating Instructions 28 9639 77 AA 6 Troubleshooting 57 6 1 How to get help 57 7 Reference information 59 7 1 Instrument specifications 59 7 2 Ordering information 60 ...

Page 5: ...onal computer running Microsoft Windows in the version provided with your product You should be acquainted with the use of general laboratory equipment and with handling of biological materials You must read the Safety Instructions in Chapter 2 of these Operating Instructions The system should be installed according to the instructions in Chapter 3 of these Operating Instructions You should unders...

Page 6: ...eraction studies in research applications The MicroCal iTC200 system is intended for research use only and shall not be used in any clinical procedures or for diagnostic purposes Safety notices These Operating Instructions contain WARNINGS CAUTIONS and NOTICES concerning the use of the product with meanings as defined below WARNING WARNING indicates a hazardous situation which if not avoided could...

Page 7: ...the directives and standards that are fulfilled by MicroCal iTC200 Manufacturing information CE conformity Requirement Content Name and address of manufacturer GE Healthcare MicroCal Products Group 22 Industrial Drive East Northampton Massachusetts 01060 USA Place and date of declaration Northampton Massachusetts USA Jan 2010 Identity of person authorized to sign Declaration of Conformity See EC D...

Page 8: ...ns described in the user documentation or explicitly authorized by GE Healthcare 2004 108 EC ElectroMagnetic Compatibility EMC Directive Directive Title Standard Description Notes EN 61010 1 IEC 61010 1 CAN CSA C22 2 no 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use EN 61326 1 CISPR Group 1 Class A EMC emissions and immunity requirements for measure...

Page 9: ...gulatory information 1 2 Regulatory compliance of connected equipment Any equipment connected to the MicroCal iTC200 should meet the safety requirements of EN 61010 1 IEC61010 1 or relevant harmonized standards Within the European Union connected equipment must be CE marked ...

Page 10: ...voided Sample and reference cells are accessible for filling and cleaning through the top of the unit The sample cell is on the left as one faces the front of the unit A pair of identical coin shaped cells is enclosed within two shields the inner shield is referred to as the jacket Access stems travel from the top exterior of the instrument to the cells Both the coin shaped cells and the access st...

Page 11: ...ical experiment the syringe containing a ligand is titrated injected into the cell containing a solution of macromolecule An injection which results in the evolution of heat exothermic within the sample cell causes a negative change in the DP power since the heat evolved chemically provides heat that the DP feedback is no longer required to provide The opposite is true for endothermic reactions Si...

Page 12: ...lace under computer control The user inputs the experimental parameters temperature number of injections injection volumes and the computer carries out the experiment DoNotTranslate_GE_Color Origin software is then used to analyze the ITC data using fitting models to calculate reaction stoichiometry n binding constant KA enthalpy ΔH and entropy ΔS ...

Page 13: ... is not attached or not turned on the program will open into Demo Mode in which the user can see and manipulate the program but it will not attempt to control the MicroCal iTC200 To the right of the status bar the Time Left box during a run will show the time left until the end of the run When the software is first started the Experimental Design tab is selected this contains the simple run contro...

Page 14: ...nly and should not be used for data analysis Users should open a separate copy of DoNotTranslate_GE_Color Origin for MicroCal iTC200 to perform data analysis Pictured below is the main DoNotTranslate_GE_Color Origin window for MicroCal iTC200 data display Figure 1 4 DoNotTranslate_GE_Color Origin Real Time display The ITC cell status the MicroCal iTC200 numeric display and the buttons for MicroCal...

Page 15: ...Cal iTC200 Operating Instructions 28 9639 77 AA 15 Introduction 1 Control software 1 4 time tools in DoNotTranslate_GE_Color Origin refer to the MicroCal iTC200 Experimental and Data Analysis Tutorial Guide ...

Page 16: ...1 Introduction 1 4 Control software 16 MicroCal iTC200 Operating Instructions 28 9639 77 AA ...

Page 17: ... operating this instrument All instrument modifications should be performed only by personnel trained by GE Healthcare Equipment damage personal injury or even death may result if this equipment is operated altered or maintained by untrained personnel or in an irresponsible or improper manner 2 1 Safety precautions Introduction Before installing operating of maintaining the system you must be awar...

Page 18: ...an described in the MicroCal iTC200 and or Auto MicroCal iTC200 manuals WARNING Make sure the rear power connector is always accessible WARNING Use caution when using solutions near the instrument If any liquid is spilled on or around the instrument unplug the instrument immediately and wipe it up If there is any possibility that liquid may have leaked into the instrument case contact GE Healthcar...

Page 19: ...ses and other personal protective equipment appropriate with the current application to ensure personal safety during operation WARNING The operator should always follow proper laboratory procedures in handling and disposing of volatile or hazardous solutions WARNING This instrument is used for a wide variety of experiments that can utilize potentially hazardous materials Use of these could cause ...

Page 20: ...e shipment WARNING Replace fuses ONLY with 4 00 Amp 250 Volt Time Delay Fuses Several spare fuses are provided with the original shipment WARNING Access to power switch and power cord Do not block the rear and side panel of the instrument The Power switch must always be easy to access The power cord must always be easy to disconnect NOTICE Disconnect power To prevent equipment damage always discon...

Page 21: ...es before an experiment so this does not affect normal operation of the instrument NOTICE The MicroCal iTC200 instrument should always be moved in its normal operating orientation Other orientations will subject delicate sensors inside the instrument to stress WARNING Replace fuses ONLY with same type fuses Several spare fuses are provided with the original shipment and the power receptacle is lab...

Page 22: ... the entire system tubing with distilled water before service and maintenance WARNING Hazardous chemicals during maintenance When using hazardous chemicals for cleaning wash the system with a neutral solution in the last phase or step WARNING Decontaminate the equipment before decommissioning to ensure the removal of all hazardous residues WARNING CONTRAD 70 Decon 90 is corrosive and therefore dan...

Page 23: ...s on the instrument The illustration below shows an example of the identification labels attached to the rear of the MicroCal iTC200 instrument Figure 2 1 Back panel of instrument Labels on the USB hub The illustration below shows an example of the label on the back of the USB hub Figure 2 2 USB hub label ...

Page 24: ... 22 MicroCal iTC200 Operating Instructions 28 9639 77 AA Labels on the Washing Module The illustration below shows an example of the identification labels attached to the rear of the Washing Module Figure 2 3 Labels on Washing Module ...

Page 25: ...before using the system Do not open any covers or replace parts unless specifically stated in the user manual The system complies with applicable European directives This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact and authorized of the manufacturer for information concerning...

Page 26: ...00 Operating Instructions 28 9639 77 AA Controller The controller shuts down in an undefined state The MicroCal iTC200 run is interrupted immediately in an undefined state Washing module The washing module shuts down immediately in an undefined state ...

Page 27: ...he different materials must be separated and recycled according to national and local environmental regulations Recycling of hazardous substances The MicroCal iTC200 instrument contains hazardous substances Detailed information is available from your GE Healthcare representative Disposal of electrical components Waste of electrical and electronic equipment must not be disposed as unsorted municipa...

Page 28: ...2 Safety instructions 2 3 Recycling procedures 26 MicroCal iTC200 Operating Instructions 28 9639 77 AA ...

Page 29: ...h space ca 70 cm wide This location should be away from strong drafts room temperature fluctuations intense sunlight vibrations and strong electrical or magnetic fields as may be produced by an NMR microwave oven large motors or refrigeration units In addition the mains power source 100 to 240 VAC should be properly grounded and free from voltage fluctuations harmonic distortions power dips and sp...

Page 30: ...ngineer Table 3 1 Power supply requirements It is emphasized that room temperature fluctuations i e maximum 2 5 ºC due to the cycling on off of heating and cooling systems strong air currents sunlight directly on the instrument and through space electromagnetic waves may cause subtle performance problems Table 3 2 Environmental operating requirements AC Mains Requirements Specification Requirement...

Page 31: ... great care Check the equipment for damage before starting assembly and installation Document any damage and contact your local GE Healthcare representative 3 4 Set up Arrange the components on the desktop similar to the picture of the system see Figure 3 1 The computer may be on either side depending on convenience Do not place the wash station into position yet Follow the instructions below for ...

Page 32: ...washers on the top of the instrument used to mount the injection tower Figure 3 2 MicroCal iTC200 with washing module Part Description 1 Cell cleaning adaptor 2 Washing module 3 Solution tubes Waste Water Methanol Buffer 4 Cell port 5 Fill port adaptor 6 Pipette 7 Pipette tower 8 Syringe washing and drying station 9 Tube holder 1 2 3 4 5 6 7 8 9 ...

Page 33: ...eve acceptable noise levels Hold the injection tower down firmly with one hand while tightening the screws with the other 7 Plug the pipette connector to the back of the injector tower riser and tighten the lock screws with a 0 05 inch hex driver 1 3mm 8 Lift the black locking clip on the front of the instrument on the interconnection board behind the faceplate Insert the ribbon cable from the inj...

Page 34: ...ble to the labeled Controller PC USB port Figure 3 3 Controller connector to PC A 2 Connect the USB type A ends of two USB cables to the hub as shown in Figure 3 5 Figure 3 4 USB connectors 3 Connect the USB type B ends to the USB 1 and USB 2 connectors on the rear of the MicroCal iTC200 instrument Fig 3 6 4 Place the Washing Module on top of the MicroCal iTC200 cell 5 Connect the USB type A end f...

Page 35: ... 28 9639 77 AA 33 Installation 3 Set up 3 4 6 Connect the green grounding strap wire between the Washing Module and the MicroCal iTC200 cell Figure 3 5 Washing Module and MicroCal iTC200 cell Note 1 in Figure 3 5 is the power cord grommet 1 ...

Page 36: ...d a Ground Fault Circuit Interrupter GFCI Figure 3 6 MicroCal iTC200 view of back plate Washing module Connect the power cord from the power supply to the power receptacle see Figure 3 7 on the rear of the Washing module see Figure 3 6 Power Connect the power supply WARNING To enhance safety always plug the instrument into a Ground Fault Circuit Interrupter GFCI device Part Description Part Descri...

Page 37: ...up 3 4 only to a main power supply receptacle with a 3 wire protective Earth ground and a Ground Fault Circuit Interrupter GFCI Figure 3 7 Washing module power supply unit WARNING To enhance safety always plug the instrument into a Ground Fault Circuit Interrupter GFCI device ...

Page 38: ...ons Figure 3 8 Washing Module pipette wash station connections Attach Washing Module Tubing C1 to syringe fill port Figure 3 8 C2 to needle wash port Figure 3 8 C3 to top of cleaning device Figure 3 9 C4 to side of cleaning device Figure 3 9 C5 to waste Figure 3 9 Figure 3 9 Cell tool connections ...

Page 39: ...into its slot Figure 3 10 Solution vials Table 3 4 Solution vials Note If buffer is not desired for some reason this vial may also be filled with water 3 5 Validation After installation it is recommended that a titration of a known system be performed to test that the instrument has been installed correctly Part Description Part Description 1 Vacuum vial opaque color 3 Methanol vial 2 Distilled wa...

Page 40: ...tallation 1 Click on the START button on the lower left side of the screen and then on Control Panel 2 In the Control Panel window click on Add or Remove Programs 3 Find InitDTSetup in the program list Click the Remove button by it In the next window click Yes to confirm the removal Special Permissions The MicroCal iTC200 software and Origin both need to be able to write data into their own folder...

Page 41: ...atically ensures that the system is does not incur any damage and keeps the MicroCal iTC200 cell ready Periods of inactivity GE Healthcare recommends that the MicroCal iTC200 application be closed and the master power be turned off when the system will not be used for extended periods of time 4 2 Basics of performing a run In order to perform a basic ITC titration experiment the user must load the...

Page 42: ...leaning and drying of the syringe In the Instrument Controls tab click Syringe Fill The software will prompt the user to move the pipette as necessary and the Washing Module will fill the syringe See sections 4 4 to 4 7 for more information 4 4 Loading the cell To load the cell do the following 1 To load the sample cell gently insert the glass Hamilton syringe into the left sample cell until it to...

Page 43: ...ift the tip of the syringe to the cell port just below the visible portion of the cell port and find the ledge See Figure 4 3 that is formed where the cell stem meets the cell port Place the syringe on the ledge at the top of the metal cell stem and remove the excess solution If the reference cell needs refilling follow the same procedure as for the sample cell Figure 4 3 Schematic representation ...

Page 44: ...shed the syringe and sample cell should be cleaned as soon as possible Procedures after a run The MicroCal iTC200 was designed to have its power on for extended periods of time This will keep the system electronics at the normal operating temperature It is recommended that the power of the MicroCal iTC200 cell be turned off during extended periods of down time such as holidays and vacations After ...

Page 45: ...he simulation window will update with a rough graph and the Results column at the right of the screen will have values for the cell and syringe concentrations The calculated C value is listed below its background is color coded The C value predicts the shape or sigmoidicity of the curve Optimal values for C are between 5 and 500 green values between 1 and 5 and 500 and 1000 should work but may not...

Page 46: ...file name has been entered The software will double check with the user before allowing any files to be overwritten The Stop button which is available only during a run will abort the run immediately The Thermostat Control section allows for setting of the thermostat temperature which will be maintained during the MicroCal iTC200 thermostatic idle state Pre thermostatting the MicroCal iTC200 and s...

Page 47: ... syringe The program then executes the Syringe Wash Short procedure The syringe cleaning takes about nine minutes Once that has completed the syringe may be loaded with sample Leave the tubing connected to the syringe Leave the syringe in the wash position or the rest position Click Syringe Load The plunger will move to the bottom of the syringe to prepare for loading This must be performed with t...

Page 48: ...oximately 8 minutes It will rinse the syringe first with water and then with methanol It will then pull vacuum through the syringe for several minutes to dry it Connect the syringe tubing to the syringe fill port Click Syringe Wash Long The software will prompt the user to make sure that the tubing is connected Click OK The Washing Module will perform a more thorough washing routine approximately ...

Page 49: ...the system electronics at the normal operating temperature It is recommended that the power of the MicroCal iTC200 cell be turned off during extended periods of down time such as holidays and vacations After use clean the sample cell following procedure in Section 4 7 and fill the sample cell with distilled water Wash and dry the syringe using procedure in section Section 4 7 NOTICE For quick star...

Page 50: ...4 Operation 4 10 Procedures after a run 48 MicroCal iTC200 Operating Instructions 28 9639 77 AA ...

Page 51: ...illing every week or so is sufficient The mildest method of cleaning the sample cell is simply to rinse the cell with buffer several times before loading the sample This should be done whenever the necessary buffer is available After every few runs or after any sample that precipitates the sample cell should be cleaned using the Washing Module Run the appropriate Washing Module sequence and follow...

Page 52: ...yringe in the pipette 6 Once the nut is loose move the pipette to the loading hole 7 Use the manual pipette controls to lower the pipette tip about 0 3 inches Without the bottom nut the syringe will move down with the pipette tip 8 Pull down on the syringe gently until it slides down into the storage case The soft grip tweezers may help with this 9 Move the pipette out of the way and screw the cap...

Page 53: ...Be very careful as if the syringe is not all the way up the needle will catch on the edge of the storage case likely bending the needle and making it unusable 6 Move the pipette to the cleaning hole and turn the inner metal syringe holder to the left several turns so that the bottom nut is engaged but not fully tightened 7 Move the pipette to the loading hole and insert the fill port adapter Make ...

Page 54: ... to decrease the likelihood of bending the syringe It may be difficult to insert the end of the wire from the glass bore into the metal needle good light and a magnifying glass will help Continue to insert the wire until it emerges from the tip of the needle Carefully pull the wire back through the needle and glass bore Figure 5 3 Wire in needle 5 5 Removing pipette The pipette is held by the arm ...

Page 55: ...tab click the Remove Old Tip button to position the tip 2 Use the blade of the X acto knife provided with the instrument to make a diagonal cut on the side of the white Teflon tip See Figure 5 5 and use the small tipped tweezers to pull the tip off See Figure 5 5 3 Click the Install New Tip button to position the tip 4 Use the tip pusher tool with the new tip in the slot hole side up and firmly pr...

Page 56: ...wn buttons to move the plunger tip to just above the fill port in the upper side of the glass syringe bore Once the position is correct click the Calibrate Open Fill Port button to have the software set the current location as the open fill port location Click the Open and Close buttons in the bottom section to check the calibration 5 8 Y axis calibration check It is recommended that the y axis ca...

Page 57: ...tion phase has completed the initial delay will begin and the pulses will be applied as entered As each pulse completes Origin will analyze the pulse region and determine the deflection of the baseline as well as the energy area of the pulse The requested power and energy will also be displayed as will a percent error for both power and energy The reported error in deflection or energy should be l...

Page 58: ...nt below the power switch and above the plug If the fuses repeatedly blow unplug the instrument and contact your local GE Healthcare representative WARNING Always disconnect power supply from the instrument before replacing fuses WARNING Replace fuses ONLY with same type and of fuse and rating Several spare fuses are supplied with the original shipment and the power receptacle is labeled with the ...

Page 59: ...l service representative The second and less extreme general category of a problem is when an MicroCal iTC200 instrument is functioning but is not operating within its normal performance specifications Large baseline drifting non repeatable control peaks water water and or an increase in short term noise level are examples of performance problems These problems may be corrected by the operator in ...

Page 60: ... water If after completion of the steps listed above the MicroCal iTC200 performance is not corrected please contact the service department for help The water runs should be provided to the MicroCal service technician for evaluation Following the evaluation a representative from the service department will contact you with comments and recommendations ...

Page 61: ...re another 25 cm of bench space Characteristic Data Operating Temperature Range 2 C to 80 C Response Time 10 seconds Cell Design 200 µL coin shaped Titration Syringe 40 µL Maximum Usable Volume 35 µL Smallest Injection Size 0 1 µL Stirring Rate 500 to 1500 r p m Description Data Cell Material Hastelloy Alloy C 276 Dimensions Calorimeter 16 5 33 26 7 cm 6 13 10 Controller 39 4 41 9 19 1 cm 15 16 7 ...

Page 62: ...Voltage 100 to 240 Volts AC Frequency 50 60 Hz Power 70 Watts Fuses 2 4 00 A 250 V Time Delay Output Secondary Data connection only Protective Earth Terminals Internal external marking Mode of Operation Continuous Classification Class I Condition Characteristic Limits Operation Temperature 10 C to 28 C Humidity 0 to 70 RH non condensing Atmospheric Pressure 700 to 1060 hPa Storage no liquid in cel...

Page 63: ......

Page 64: ...al Electric Company All rights reserved First published Jan 2010 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare UK Ltd Amersham Place Little Chalfont Buckingha...

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