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5.4

Transfer protocol

In this section

This section describes how to transfer the proteins from the gel to a blotting
membrane using tank transfer and semi-dry blotting.

Tank transfer

Transfer buffer

Tris-glycine buffer: 25 mM Tris, 192 mM glycine, 20% methanol. Prepare
fresh.

Use a pre-chilled transfer buffer.

Note:

Preparing the membrane

Action

Step

Pre-wet the membranes:

•

NitroCellulose (NC) membrane: Pre-wet the membranes in
distilled water for five minutes followed by 10 minutes in
transfer buffer.

•

PVDF membrane: Pre-wet the PVDF membranes first in
methanol for 20 seconds, then in distilled water for 5 minutes
and finally, in cold transfer buffer for at least 10 minutes.

Note:

Use a pre-cut membrane provided by GE Healthcare. See

Chapter 8

Related products, on page 62

Amersham ECL Gel and Gel Box User Manual 28-9943-33 AC

5 Protocols

5.4 Transfer protocol

Summary of Contents for Amersham ECl Gel Box

Page 1: ...GE Healthcare Life Sciences Amersham ECL Gel Box User Manual ...

Page 2: ......

Page 3: ...22 2 4 Declaration of Hazardous Substances DoHS 26 3 System description 27 3 1 Amersham ECL Gel Box 30 3 2 Amersham ECL Gel 34 4 Installation 38 5 Protocols 39 5 1 Electrophoresis protocol 44 5 2 Removing the gel from the cassette 46 5 3 Post staining of the gel 53 5 4 Transfer protocol 57 6 Maintenance 59 7 Troubleshooting 62 8 Related products Amersham ECL Gel and Gel Box User Manual 28 9943 33 ...

Page 4: ......

Page 5: ...rotein detection protocols and has an extended shelf life for increased convenience Scope of this document These instructions provide information on how to perform polyacrylamide gel electrophoresis PAGE using Amersham ECL Gel Box and Amersham ECL Gel Additionally protocols for post staining and transfer are provided In this chapter This chapter contains the following sections See page Section 6 1...

Page 6: ...ng PAGE followed by either Western blotting or post staining detection methods Western blotting detection method Post staining detection method Transfer Detection Post staining Detection Gel electrophoresis 6 Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 1 Introduction 1 1 Protein gel electrophoresis overview ...

Page 7: ...of Amersham ECL Gel Box See definitions below Warnings WARNING WARNING indicates a hazardous situation which if not avoided could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood Cautions CAUTION CAUTION indicates a hazardous situation which if not avoided could result in minor or moderate injury It is important not to proc...

Page 8: ...OTICE NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment 8 Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 1 Introduction 1 2 Important user information ...

Page 9: ...ive LVD 2006 95 EC Electromagnetic Compatibility EMC Directive 2004 108 EC CE Marking The CE marking and the corresponding declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in the user documentation and used in the same state as it was delivere...

Page 10: ...assemblies for electricalmeasurementandtest EN 61010 031 IEC 61010 031 Harmonized with 2004 108 EC EMC emissions and immunity requirements for electrical equipment for measurement control and laboratory use EN 61326 1 Regulatory compliance of connected equipment Any equipment connected to Amersham ECL Gel Box should meet the safety requirements of EN 61010 1 IEC 61010 1 or other relevant harmonize...

Page 11: ...nformation regarding recycling are also included in this chapter In this chapter This chapter contains the following sections See page Section 12 2 1 Safety precautions 18 2 2 Labels 21 2 3 Recycling information 22 2 4 Declaration of Hazardous Substances DoHS Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 11 2 Safety ...

Page 12: ...ly trained personnel only CAUTION Do not use any accessories not supplied or recommended by GE Healthcare Installation WARNING Access to power switch and power cord Do not block access to the Power switch on the Power supply and the Power cord The Power switch must always be easy to access The Power cord must always be easy to disconnect 12 Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 2 ...

Page 13: ...RNING High voltage Before use check that Amersham ECL Gel Box is completely dry on the outside before connecting it to a power supply Wipe dry with a cloth WARNING Do not use Amersham ECL Gel Box if it is not working properly nor if it has suffered any damage for example damage caused by dropping the equipment damage by spilling liquid onto it Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC...

Page 14: ...ys switch off the power supply and disconnect Amersham ECL Gel Box from the power supply before removing the safety lid WARNING Do not operate Amersham ECL Gel Box without a full functioning safety lid in place WARNING Do not exceed the maximum operating voltage of 200 V WARNING Do not operate the Amersham ECL Gel Box inside a metal tray 14 Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 2 ...

Page 15: ...t operate Amersham ECL Gel Box close to strong magnetic fields CAUTION Hazardous substances When using hazardous chemicals take all suitable protective measures such as wearing protective glasses and gloves resistant to the substances used Follow local and or national regulations for safe operation CAUTION Do not move Amersham ECL Gel Box during operation Amersham ECL Gel and Gel Box User Manual 2...

Page 16: ... disconnect Amersham ECL Gel Box from the Power supply before maintenance and cleaning WARNING Cleaning Do not autoclave bake or microwave Amersham ECL Gel Box WARNING Cleaning Do not wash Amersham ECL Gel Box in a mechanical washer WARNING Cleaning Do not use abrasive creams or scourers 16 Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 2 Safety 2 1 Safety precautions ...

Page 17: ...h a soft damp tissue Let the instrument dry completely before use Materials Safety Data Sheet It is recommended to read the Materials Safety Data Sheet MSDS before using Amersham ECL Gel Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 17 2 Safety 2 1 Safety precautions ...

Page 18: ...on below shows an example of the label that is attached to the bottom of the Amersham ECL Gel Box Safety symbols The following safety symbols are used in the label Meaning Label Warning Read the user documentationbefore using the instrument Do not open any covers or replace parts unless specifically stated in the user documentation 18 Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 2 Safety...

Page 19: ...ent The instrument complies with the requirements for electromagnetic compliance EMC in Australia and New Zealand N3732 The instrument complies with applicable European directives The instrument is listed according to TÜV Rheinland The instrument is protected throughout by double insulation Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 19 2 Safety 2 2 Labels ...

Page 20: ...rately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment This symbol indicates that the product contains hazardous materials in excess of the limits established by the Chinese standard SJ T11363 2006 Requirements for Concentration Limits for Certain Hazardous Substances in Electronic Information Product 20 Amersham ECL Gel a...

Page 21: ... different materials must be separated and recycled according to national and local environmental regulations Disposal of electrical components Waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact anauthorizedrepresentativeofthemanufacturerforinformationconcerning the decommissioning of equipment Amersham ECL ...

Page 22: ...ymbol is the Environment friendly Use Period EFUP which indicates the period during which the toxic or hazardous substances or elements contained in electronic information products will not leak or mutate under normal operating conditions so that the use of such electronic information products will not result in any severe environmental pollution any bodily injury or damage to any assets The unit ...

Page 23: ...e collected separately and handled properly after decommissioning 该标志表明本产品含有超过SJ T11363 2006 电子信息产品中有毒有害物质的限量要求中限量的有毒有害物质标志中的数字为本产品的环保使用期表明本产品在正常使用的条件下有毒有害物质不会发生外泄或突变用户使用本产品不会对环境造成严重污染或对其人身财产造成严重损害的期限单位为年为保证所申明的环保使用期限应按产品手册中所规定的环境条件和方法进行正常使用并严格遵守产品维修手册中规定的期维修和保养要求产品中的消耗件和某些零部件可能有其单独的环保使用期限标志并且其环保使用期限有可能比整个产品本身的环保使用期限短应到期按产品维修程序更换那些消耗件和零部件以保证所申明的整个产品的环保使用期限本产品在使用寿命结束时不可...

Page 24: ... this part is above the limit requirement in SJ T11363 2006 X Data listed in the table represents best information available at the time of publication Applications of hazardous substances in this medical device are required to achieve its intended clinical uses and or to provide better protection to human beings and or to environment due to lack of reasonably eco nomically or technically availabl...

Page 25: ... 0 0 Amersham ECL Gel Box 28 9902 521 1 The product has not been tested as per the Chinese standard SJ T11363 2006 Requirements for Concentration Limits for Certain Hazardous Substances in Electronic Information Product Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 25 2 Safety 2 4 Declaration of Hazardous Substances DoHS ...

Page 26: ...he features of Amersham ECL Gel Box and Amersham ECL Gel In this chapter This chapter contains the following sections See page Section 27 3 1 Amersham ECL Gel Box 30 3 2 Amersham ECL Gel 26 Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 3 System description ...

Page 27: ... of Amersham ECL Gel Box 5 4 2 1 3 Description Part Description Part Cathode located under neath the plastic case 4 Power cord to external power supply 1 Buffer tanks for running buffer 5 Anode locatedunderneath the plastic case 2 Indicator light 3 Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 27 3 System description 3 1 Amersham ECL Gel Box ...

Page 28: ...perating temperature EPS 301 Recommended power supply 20 W Maximum power 100 mA Maximum current IPX5 Degree of protection ECL Gel cassette The image below shows the Amersham ECL Gel cassette 1 2 3 Description Part Description Part Cassette 3 Comb 1 Well container 2 28 Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 3 System description 3 1 Amersham ECL Gel Box ...

Page 29: ...ped with a safety lid which shall be placed on top of the base unit before a power supply is connected The safety lid has two magnets which will activate current only when their magnetic fields are in contact with the electrodes inside the base unit of Amersham ECL Gel Box This means that Amersham ECL Gel Box is only powered when the safety lid is in the correct position Removing the safety lid wi...

Page 30: ... ECL Gel Box Gel concentrations Amersham ECL Gels are available as homogenous and gradient gels in the following concentrations 10 12 4 12 8 16 4 20 For information regarding separation ranges for each Amersham ECL Gel see Separation range on page 31 30 Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 3 System description 3 2 Amersham ECL Gel ...

Page 31: ...ular weight for each Amersham ECL Gel 250 130 95 72 55 36 28 17 250 130 95 72 55 36 28 17 250 130 95 72 55 36 28 17 10 250 130 95 72 55 36 28 17 10 250 130 95 72 55 36 28 17 10 10 12 4 12 8 16 4 20 Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 31 3 System description 3 2 Amersham ECL Gel ...

Page 32: ...re available Sample well configuration 100 μl 35 μl 20 μl Sample well volume 2 wells 10 wells 15 wells 4 Stacking gel Tris HCl Buffer system in gel 25 mM Tris 192 mM glycine 0 1 SDS pH 8 3 25 mM Tris 192 mM glycine pH 8 3 Running buffer Denatured conditions Native conditions e g Tris HCl SDS or any other buffer suitable for the application Sample buffer 12 months from date of manufacture Shelf lif...

Page 33: ... 2 Make up to 10 ml with distilled water 2 SDS Sample buffer Amersham ECL Gel Running Buffer 10 contains 250 mM Tris HCl 1 92 M glycine 1 SDS Amersham ECL Gel Running buffer 1 Prepare 1 buffer for denatured conditions by adding distilled water in a 1 10 dilution Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 33 3 System description 3 2 Amersham ECL Gel ...

Page 34: ...er supply and the Power cord The Power switch must always be easy to access The Power cord must always be easy to disconnect CAUTION During electrophoresis very low quantities of gases are produced at the electrodes Make sure that Amersham ECL Gel Box is run in a well ventilated area CAUTION Amersham ECL Gel Box is designed for indoor use only 34 Amersham ECL Gel and Gel Box User Manual 28 9943 33...

Page 35: ... requirements for 200 V DC for example EPS 301 If a power supply with Ø 4 mm deep socket 6 mm internal distance between surface and socket is used it is recommended to use the adapter delivered with Amersham ECL Gel Box For mountinginstructions see Mounting the adapter on page 37 Amersham ECL Gel Box is delivered with two adapters identical apart from color one black and one red shown above Note A...

Page 36: ...l Box Without Ø 4 mm rigid insulation inner dis tance 6 mm 18 1129 59 High voltage power supplies with Ø 2 mm rigid insulation Adapter set 4 mm 2 mm for use with 4 mm HV leads to 2 mm power supply output socket EPS 601 EPS 1001 EPS 3501XL Not compatible GEHC EPS power supply with built in sudden load change detection 36 Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 4 Installation ...

Page 37: ...apter in the power supply output socket 2 WARNING Do not use the adapter in Ø 4 mm socket with 6 mm internal distance between surface and socket 6 mm Tighten the set screw using the 1 5 mm Hex wrench provided with the Amersham ECL Gel Box to lock the expandable plug of the adapter into the socket 3 Make sure that the adapter is locked into the socket 4 Amersham ECL Gel and Gel Box User Manual 28 9...

Page 38: ... protocols for post staining and transfer are provided In this chapter This chapter contains the following sections See page Section 39 5 1 Electrophoresis protocol 44 5 2 Removing the gel from the cassette 46 5 3 Post staining of the gel 53 5 4 Transfer protocol 38 Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 5 Protocols ...

Page 39: ...ng properly nor if it has suffered any damage for example damage caused by dropping the equipment damage by spilling liquid onto it WARNING Do not connect the high voltage cable to an external power supply if it is not working properly nor if it has suffered any damage for example damage to its plug or cable Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 39 5 Protocols 5 1 Electrophoresis ...

Page 40: ...legs of the cassette 4 Place Amersham ECL Gel in Amersham ECL Gel Box so that the well side of the cassette faces toward the cathode and the other cassette leg faces toward the anode 5 Place the safety lid on top of Amersham ECL Gel Box 6 Connect Amersham ECL Gel Box to the power supply EPS 301 and pre run the gel for 12 minutes at 160 V 7 WARNING Do not exceed the maximum operating voltage of 200...

Page 41: ...ilable for sample loading 10 Note Small gel pieces can be detached from the well container This will not affect gel performance Note Do not discard the comb Add 6 ml of 1 running buffer to the well container 11 Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 41 5 Protocols 5 1 Electrophoresis protocol ...

Page 42: ...oad the samples directly into the wells in the gel Note A maximum of 0 5 μg band per well of sample can be loaded Overloading may cause smearing and distortion 3 Note To ensure uniform mobility load an equal volume of 1 SDS sample buffer into any unused well Place the safety lid on top of Amersham ECL Gel Box 4 Running conditions Run the gel according to the recommendations below Value Parameter 1...

Page 43: ...Value Parameter 60 minutes Run time WARNING Do not exceed the maximum operating voltage of 200 V Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 43 5 Protocols 5 1 Electrophoresis protocol ...

Page 44: ...e the run is completed shut off the power disconnect the electrodes remove the safety lid and finally remove the gel cassette from Amersham ECL Gel Box 1 Open the cassette by inserting the edge of the comb in the slot opposite the sample wells and twist 2 Remove the top plate from the gel cassette and allow the gel to sit on the bottom plate 3 44 Amersham ECL Gel and Gel Box User Manual 28 9943 33...

Page 45: ...gel to remove the front 4 Hold the gel cassette bottom plate over a container with suitable buffer with the gel facing downwards Gently push a tweezer between the gel and the cassette bottom plate until the gel is removed from the bottom plate 5 Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 45 5 Protocols 5 2 Removing the gel from the cassette ...

Page 46: ...ns 250 ml of each solution is used in each step Preparation Solution 1 Mix 400 ml of ethanol and 100 ml of acetic acid with distilled water 2 Make up to 1 0 l with distilled water Fixing solution 1 Mix 1 tablet of PhastGel Blue R 350 with destaining solution 2 Make up to 400 ml with destaining solution Heat to 60 C stirring constantly and filter before use Staining solution Distilled water Washing...

Page 47: ... SDS to diffuse out of the gel 1 Staining Discard the fixing solution Stain the gel for 10 minutes in staining solution Cover the staining dish 2 Washing Rinse the gel once in distilled water 3 Destaining Destain the gel by changing the destaining solution several times until the stained protein bands are clearly visible against the clear background 4 Preserving Soak the destained gel in preservin...

Page 48: ...x months Fixating Acidifying solution 1 Disolve 6 2 g boric acid in 800 ml distilled water Adjust to pH 10 5 with NaOH 2 Make up to 1 0 l with distilled water Note Boric acid sodium hydroxide buffer can be stored at room temperature for up to six months Working staining solution Note Staining solution should be made fresh just before use by adding 1 part Deep Purple to 200 parts borate buffer for ...

Page 49: ...ng Fix gels from 90 minutes to overnight in fixing solution 1 Staining Stain for 90 minutes at room temperature covered from light using the prepared working staining solution Prior to diluting with borate buffer ensure that the Deep Purple concentrate is at room temperature 2 Washing Wash for 90 minutes in washing solution 3 Acidifying Acidify gels for 45 minutes Further washes in this solution c...

Page 50: ...nformation Silver staining solutions Preparations Solutions 1 Mix 75 ml ethanol and 25 ml glacial acetic acid 2 Make up to 250 ml with distilled water Fixing solution 1 Mix 75 ml ethanol 10 ml sodium thiosulfate 5 w v and 1 packet of sodium acetate 17 g 2 Make up to to 250 ml with distilled water Before use Add 1 25 ml glutaraldehyde 25 w v Sensitizing solution 1 Mix 25 ml of silver nitrate soluti...

Page 51: ... ml of glycerol 87 w w 2 Make up to 250 ml with distilled water Preserving solution Silver staining protocol Action Step Fixation Soak the gel in fixing solution for 120 minutes or overnight 1 Sensitizing Discard the solution Add sensitizing solution and gently shake for at least 120 minutes 2 Washing Remove the sensitizing solution Add distilled water and wash five times for 15 minutes each time ...

Page 52: ... have reached desired intensity Note If the background and and the bands are too strong use a cold transfer buffer and or transfer the gel to stopping solution before desired density has been reached 6 Stopping Gently shake the gel in stopping solution for 120 minutes 7 Preserving Add preserving solution and shake gently for 120 minutes 8 52 Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 5...

Page 53: ...brane Action Step Pre wet the membranes 1 NitroCellulose NC membrane Pre wet the membranes in distilled water for five minutes followed by 10 minutes in transfer buffer PVDF membrane Pre wet the PVDF membranes first in methanol for 20 seconds then in distilled water for 5 minutes and finally in cold transfer buffer for at least 10 minutes Note Use a pre cut membrane provided by GE Healthcare See C...

Page 54: ...t 3 cm of the transfer buffer 2 Build the stack with the membrane closest to the anode side 3 Below is an illustration of the transfer sandwich CATHODE ANODE Transfer direction Two buffer soaked blotting papers Foam Sponge Foam Sponge Two buffer soaked blotting papers Polyacrylamide Gel Membrane Layer 1 Place a 3 mm thick foam sponge in the transfer cassette and press gently to remove any air bubb...

Page 55: ...and ensure there are no air bubbles 9 Layer 5 Cover the gel with two pre wetted sheets of blotting paper 10 Layer 6 Finally place a sponge of 3 mm and again press gently to remove any air bubbles 11 Close the cassette and press lightly to lock the tabs 12 Place the cassette into the tank 13 Run the transfer according to the recommendations from the transfer tank supplier 14 Recommended running con...

Page 56: ...current leading to inefficient or uneven transfer of proteins Ensure that no air bubbles form when applying the gel to the membrane Bubbles will cause blank spots on the membrane where no protein transfer occurs Prolonged semi dry blotting may lead to overheating and gel drying due to buffer depletion Semi dry transfer works well for most proteins but may be less efficient for large proteins Proto...

Page 57: ...ng Do not autoclave bake or microwave Amersham ECL Gel Box WARNING Cleaning Do not wash Amersham ECL Gel Box in a mechanical washer WARNING Cleaning Do not use abrasive creams or scourers NOTICE Cleaning Clean the instrument with distilled water and wipe dry with a soft damp tissue Let the instrument dry completely before use Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 57 6 Maintenance ...

Page 58: ...fer 1 Rinse thoroughly with distilled water 2 Let the instrument air dry or wipe with a soft damp tissue 3 Keep power cables and connectors dry 4 Storage Store Amersham ECL Gel Box in a dry location at room temperature 58 Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 6 Maintenance ...

Page 59: ...t of protein Centrifuge the samples Change the pH of the sample buffer Heat the sample together with SDS Decrease the salt concentration of the sample solution using dialysis or gel filtration Sample overload Poorly soluble or weakly charged particles such as carbohydrates in sample High salt concentrationin the sample Contaminants such as membranes or DNA complexes in the sample Streaking of prot...

Page 60: ...el Remove the carbohydrate by enzymatic or chemical means Sample contains appreciable carbohydrate Use a gradient gel Try addition of a non ionic detergent Sample contains lipoproteins Start with cold buffer 15 C Excessive heating Protein denaturation and band inversion Wash the gels extensively 3 x 10 minutes with ultrapure wateranduse30 methanol to destain the gel Use 10 TCA to fix the proteins ...

Page 61: ...A at start Check that the tanks are not overfilled Check if the unit is damaged and needs to be replaced Leak between tanks with or without gel cassette Green light but only approx 35 mA at start Check that tanks are not overfilled Check if the unit is damaged and needs to be replaced Leak between tanks with gel cassette Green light but approx 65 mA at start Amersham ECL Gel and Gel Box User Manua...

Page 62: ...ays 2 D Quant Kit Amersham markers Code no Quantity Product RPN755E 250 μl Low Range Rainbow Molecular Weight Markers RPN756E 250 μl High Range Rainbow Molecular Weight Markers RPN800E 250 μl Full Range Rainbow Molecular Weight Markers RPN810 1 pack 25 loadings Amersham ECL DualVue Western Blotting Markers RPN850E 120 μl Amersham ECL Plex Fluorescent Rainbow Markers RPN851E 500 μl Amersham ECL Ple...

Page 63: ... 20 10 wells 28 9901 55 10 Amersham ECL Gel 10 15 wells 28 9901 56 10 Amersham ECL Gel 12 15 wells 28 9901 57 10 Amersham ECL Gel 4 12 15 wells 28 9901 58 10 Amersham ECL Gel 8 16 15 wells 28 9901 59 10 Amersham ECL Gel 4 20 15 wells 28 9901 60 10 Amersham ECL Gel 10 2 wells 28 9901 61 10 Amersham ECL Gel 12 2 wells 28 9901 62 10 Amersham ECL Gel 4 12 2 wells 28 9901 63 10 Amersham ECL Gel 8 16 2 ...

Page 64: ...buffers Code no Quantity Product 28 9902 52 2 x 125 ml Amersham ECL Gel Running Buffer 10 250 mM Tris HCl 1 92 M glycine 1 SDS 17 1329 01 10 g Bromophenol Blue Gel staining reagents Code no Quantity Product RPN6305 5 ml Deep Purple Total Protein Stain RPN6306 25 ml 17 1150 01 1 PlusOne Silver Staining Kit Protein 17 0518 01 40 Tablets PlusOne Coomassie Blue PhastGel R 350 Tablets 64 Amersham ECL G...

Page 65: ...x 16 cm 11 0013 42 1 TE 77 PWR Semi Dry Transfer Unit 21 x 26 cm 11 0033 95 1 Amersham ECL Multiprobe 11 0033 96 1 Amersham ECL Multiprobe XL 80 6204 26 1 TE 22 Mini Tank Transfer Unit 80 6209 58 1 TE 62 Transfer Unit Amersham ECL Gel and Gel Box User Manual 28 9943 33 AC 65 8 Related products ...

Page 66: ...5 cm Membranes RPN2020D 10 sheets Hybond ECL 20 20 cm RPN7 58D 10 sheets Hybond ECL 8 7 5 cm RPN2020LFP 10 sheets Hybond LFP 20 20 cm 28 9909 84 10 sheets Hybond LFP 8 7 5 cm RPN2020F 10 sheets Hybond P 20 x 20 cm 28 9909 83 10 sheets Hybond P 8 7 5 cm Amersham Blocking agents Code no Quantity Product RPN418 40 g ECL Prime Blocking Agent RPN2125 40 g ECL Blocking Agent 66 Amersham ECL Gel and Gel ...

Page 67: ...agment from donkey 28 9011 08 150 μg ECL Plex goat α mouse IgG Cy2 28 9011 10 150 μg ECL Plex goat α rabbit IgG Cy2 28 9011 06 150 μg ECL Plex goat α rabbit IgG Cy3 PA43009 150 μg ECL Plex goat α mouse IgG Cy3 PA45011 150 μg ECL Plex goat α rabbit IgG Cy5 PA45010 150 μg ECL Plex goat α mouse IgG Cy5 Detection reagents Code no Quantity Product RPN2235 for 1000 cm2 membrane Amersham ECL Select Weste...

Page 68: ...conjugated Antibodies Code no Quantity Product RPN998 1 Amersham ECL Plex Western Blotting CombinationPack Cy3 Cy5 Hybond ECL RPN999 1 Amersham ECL Plex Western Blotting CombinationPack Cy3 Cy5 Hybond LFP for two slab gels Autoradiography Films Code no Quantity Product 28 9068 35 50 sheets Amersham Hyperfilm ECL 5 x 7 inches 28 9068 36 50 sheets Amersham Hyperfilm ECL 18 x 24 cm 68 Amersham ECL Ge...

Page 69: ...eQuant LAS 500 Software and Accessories Code no Product CD and getting started 28 9380 94 ImageQuant TL 7 0 and ImageQuant TL SecurITy 8 0 Software package with Getting Started Guide Licenses for ImageQuant TL only 28 9236 62 ImageQuant TL single user license Licenses for ImageQuant TL v7 01 and IQTL SecurITy v8 0 28 9332 73 ImageQuant TL 7 01 and ImageQuant TL SecurITy 8 0 single user license Ame...

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Page 72: ...nd conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation...

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