GE Amersham ECl Gel Box, User Manual

Page: 40 / 72

Preparations before a
run
Action Step
Prepare 1× running buffer by diluting 19 ml of Amersham ECL
Gel Running Buffer, 10× in 171 ml water. 190 ml buffer is sufficient
for one electrophoresis gel. 1
Add 90 ml of 1× running buffer to each tank of Amersham ECL
Gel Box. 2
Cut open the gel package and gently remove Amersham ECL Gel
from the package. 3
Rinse the gel cassette with distilled water. Peel off the tapes from
the two legs of the cassette. 4
Place Amersham ECL Gel in Amersham ECL Gel Box so that the
well side of the cassette faces toward the cathode (-) and the
other cassette leg faces toward the anode (+). 5
Place the safety lid on top of Amersham ECL Gel Box. 6
Connect Amersham ECL Gel Box to the power supply (EPS 301)
and pre-run the gel for 12 minutes at 160 V. 7
WARNING
Do not exceed the maximum operating
voltage of 200 V
.
Note:
Expected start current approx. 45 mA.
Once the pre-run is finished, switch off the power. 8
Remove the safety lid. 9
40 Amersham ECL Gel and Gel Box User Manual 28-9943-33 AC
5 Protocols
5.1 Electrophoresis protocol