Access Array System for the Ion Torrent PGM Sequencing System: User Guide
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Chapter 1: Access Array System Overview
Amplicon tagging on the Access Array™ system significantly reduces the time required for
enrichment of targeted sequences by combining amplicon generation with library
preparation. The Access Array system workflow consists of five major phases: 1) designing
and validating target-specific primers for targeted resequencing, 2) quantifying samples, 3)
running an integrated fluidic circuit (IFC, either the LP 48.48 IFC or the Access Array 48.48
IFC), 4) attaching sequence tags and sample barcodes, and 5) qualifying and quantifying
harvested PCR products for sequencing. The chapters in this user guide detail this workflow
chronologically:
This Access Array target-specific PCR amplification scheme combines 48 target-specific (TS)
forward and reverse primers with 48 samples to generate 2,304 PCR reactions.
Design PCR primers and add tags
Qualify tagged primer sets
Select best tagged primer sets
Quantify samples
Normalize sample concentrations
Load samples and primers on Access Array IFC
PCR amplify for 35 cycles
Recover PCR products from Access Array IFC
Attach sequence tags and sample barcodes
Qualify/quantify PCR products
Select and pool samples
Clean up PCR product pool
Quantify PCR product pool for emulsion PCR
