Access Array System for the Ion Torrent PGM Sequencing System: User Guide
Chapter 1: Access Array System Overview
Access Array System Workflow
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Access Array System Workflow
The simplicity of running experiments is illustrated in the process below.
The Access Array system workflow consists of five major steps:
Materials
This section describes the materials required to perform an Access Array experiment
including the supported reagents and consumables.
Required Reagents
IMPORTANT
•
This guide is for preparing sequencing libraries with Access Array reagents.
To prepare
targeted DNA sequencing libraries with Advanta NGS Library Reagent Kits, see the
Advanta NGS Library Preparation with Access Array Protocol (PN 101-7885).
•
Store reagents according to manufacturer's storage recommendations as soon as they are
received.
Workflow Step
1
Dispense
Transfer samples and primers to the inlets on the LP 48.48 IFC (or Access Array 48.48 IFC)
from a standard 96-well plate, using an 8-channel pipette.
2
Load
Reaction mixtures are automatically loaded and assembled on the IFC using the
pre-PCR
IFC Controller AX.
3
Thermal-cycle
Perform PCR amplification using the FC1 cycler.
4
Harvest
PCR products from each sample are automatically pooled and harvested using the
post-PCR
IFC Controller AX.
5
Recover
Recover the PCR products from each of the 48 sample inlets, using an 8-channel pipette.
Quantify the sequencing library.
