Access Array System for the Ion Torrent PGM Sequencing System: User Guide
16
Chapter 2: Designing Targeted
Sequencing Primers
You can design your own Access Array target-specific primers using available online tools
(for example, UCSC website at
genome.ucsc.edu/cgi-bin/hgGateway
, Primer3 website at
bioinfo.ut.ee/primer3/
, and in silico PCR at
genome.ucsc.edu/cgi-bin/
hgPcr?hgsid=136961442
) or by using the
D3™ assay design
service at Fluidigm.
Our
D3™ assay design
service provides Access Array target-specific primers for use in
Illumina MiniSeq™, MiSeq™, NextSeq™ and HiSeq® systems and in the Ion Torrent® PGM
system. They are designed to human genomic DNA. Uniplex primers are provided in
nuclease-free water at a final volume of 100
μ
L per well in a single 96-well plate with mixed
forward and reverse primers at a final concentration of 50
μ
M. Multiplex primer sets are
provided in nuclease-free water at a final volume of 100
μ
L per well in six 96-well plates per
set at a final concentration of 60
μ
M.
If you are using primers provided by the
D3™ assay design
service, refer to the order
informatics packet for primer sequences. If you are designing your own primers, see Access
Array–Generate Tagged Primers Workbook (Fluidigm, PN 100-3873).
This guide is for preparing sequencing libraries with Access Array reagents.
To prepare
targeted DNA sequencing libraries with Advanta NGS Library Reagent Kits, see the Advanta
NGS Library Preparation with Access Array Protocol (PN 101-7885).
