Access Array System for the Ion Torrent PGM Sequencing System: User Guide
Chapter 5: Bidirectional Amplicon Tagging on the LP 48.48 IFC
Prime the IFC
27
IMPORTANT
•
Use the LP 48.48 IFC (or the Access Array 48.48 IFC) within 24 hours of opening the
package.
•
Due to different accumulator volumes, use only 48.48 syringes with 300
μ
L of control line
fluid (PN 89000020).
•
Control line fluid on the IFC or in the inlets makes the IFC unusable.
•
Load the IFC within 60 minutes of priming.
•
Be certain that the reagents 1X Access Array Harvest Solution and 1X Access Array
Hydration Reagent v2 are thawed completely to room temperature and mixed thoroughly
prior to use.
1
Inject Control Line Fluid into each accumulator on the IFC.
2
Add 500
μ
L of 1X Access Array Harvest Solution (Fluidigm, PN 100-1031) into the
H1–H4 wells on the IFC.
NOTE
If you are working with the Access Array 48.48 IFC, use 500
μ
L of 1X Access
Array Hydration Reagent v2 (blue cap, Fluidigm, PN 100-7966) instead of 1X Access
Array Harvest Solution in the H4 well. Hydration Reagent v2 ensures uniform harvest
volumes when working with the Access Array 48.48 IFC, but it is not needed when
working with the LP 48.48 IFC.
3
Remove and discard the blue protective film from the bottom of the AA IFC.
4
Load the IFC into the
pre-PCR
IFC Controller AX located in the pre-PCR lab.
5
Press
Eject
to move the tray out of the IFC Controller AX.
6
Place the IFC onto the tray by aligning the notched corner of the IFC to the A1 mark.
7
Press
Load Chip
to register the barcode of the IFC and activate the script selection.
8
Prime the LP 48.48 IFC for Access Array. Select
Prime (155x)
and
Run Script.
NOTE
For Access Array 48.48 IFCs, select
Prime (151x)
and
Run Script.
9
Once the script is complete, press
Eject
to remove the IFC.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
PRIMER INLETS
H1
H3
SAMPLE INLETS
H2
H4
INTERF
ACE ACCUMULA
T
O
R
CONT
AINMENT ACCUMULA
T
O
R
