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Recalibration
…interval [user set value] minutes:
This setting determines the frequency of recalibration. A
recalibration consists of collecting a new set of calibration images, averaging them, and producing a new
composite calibration image. This is the same process that automatically occurs at the start of an
analysis. Recalibration can be helpful if the sample in question has a tendency to adhere to the glass
surfaces of the flow cell resulting in erroneous repeat imaging of the same particles.
Context… Fluorescence/Scatter
This section allows the user to select and refine the settings for the Trigger Modes of the FlowCAM if
the unit in question is a model equipped to run in Trigger Mode. See Section Two for more information
on Trigger Mode.
Channel 1 and Channel 2:
These are the two channels that utilize PMTs to detect fluorescence signals
from particles in the sample that fluoresce (see Figure 2, letter B). The standard configuration for
Channels 1 and 2 is: Channel 1 is the >650 nm PMT and Channel 2 is the PMT for the 575 nm filter.
Scatter:
This channel uses the Scatter Detector (see Figure 2, letter H) to detect the scatter of laser light
by particles in the sample.
Each one of the above three options can be utilized in two different modes. Each channel can be set to
Trigger or Measure. When set to Trigger, the FlowCAM will be triggered to take a camera image when
a particle produces a signal (fluorescence or laser light scatter) that meets or exceeds the user defined
Threshold (see below for more information on Threshold). When set to measure, the FlowCAM will
only monitor and record the values detected on these channels but will not trigger the camera based on
