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Anatomy of a FlowCAM (Open Bench Top)
The first step in understanding the potential of the instrument is learning the components of the
FlowCAM assembly. Figure 2 is a picture of one version of the VS-IV Open Bench Top Model (this
unit can AutoImage, Scatter Trigger, has two Fluorescence Trigger channels and has a color camera.)
While this particular unit pictured below uses a 532 nm laser, and is set up to detect red (>650 nm) and
orange (575 nm) fluorescence, the principles are the same for other laser and filter configurations. An
example of such an alternative configuration would be an instrument that uses a 488 nm (blue) laser for
excitation, a green emission (525 nm) Filter set, and images with a high resolution black and white
camera. Additionally most models of the FlowCAM come equipped with a syringe pump.
Figure 2.
VS-IV Open Bench Top Series FlowCAM
A.
Digital Camera
B.
Photo-Multiplier Tubes (Channels 1 & 2)
C.
Unit and Laser Power Switches
D.
Computer
E.
Digital Signal Processsor (DSP)
F.
Pump (peristaltic)
G.
Laser Cube (laser is hidden from view)
H.
Scatter Detector (located behind cube)
I.
Flash LED (located on edge of cube)
J.
Fine Focus Adjustment
K.
Microscope Objective Cube
L.
Flow Cell Holder Mount
M.
X & Y-Axis Adjustment Knobs
N.
Course Adjustment Screw
O.
LED Collimator or Diffuser Lens cube
D
E
A
F
C
B
I
J
K
M
N
O
G
H
L
