F
LEXCELL
®
I
NTERNATIONAL
C
ORPORATION
i
TABLE OF CONTENTS
1. Getting Started .............................................................................................................. 1
Introduction
........................................................................................................................................ 1
Streamer
®
Components ...................................................................................................................... 1
Streamer
®
Setup and Assembly ......................................................................................................... 1
Sterilizing the Streamer® ................................................................................................................... 2
Streamer
®
Placement in the Incubator .............................................................................................. 3
Using
the
Pump
.................................................................................................................................. 3
Quickstart
Instructions ....................................................................................................................... 3
2. StreamSoft
™
V4.2 Software ......................................................................................... 5
Installation
Instructions
...................................................................................................................... 5
Setting up Parameters in StreamSoft
™
V4.2 ..................................................................................... 5
Main
Panel
.......................................................................................................................................... 6
General
Information
Tab
............................................................................................................. 6
System
Tab
................................................................................................................................... 7
Pre-Test
Configuration
................................................................................................................ 8
Pull-down
Menus
......................................................................................................................... 9
Operate
Menu
................................................................................................................................... 10
Manual
Mode
............................................................................................................................. 10
View
Data
.................................................................................................................................. 11
Configure
Users
......................................................................................................................... 12
Configure Regimes: Setup Parameters ..................................................................................... 13
Configure
Testing
Apparatus
.................................................................................................... 15
How to enter the proper values for your device ....................................................................... 16
Configure
System
Variables
..................................................................................................... 17
Reinitialize Hardware ................................................................................................................ 18
StreamSoft
™
V4.2 Notes .................................................................................................................. 18
3. Doing an Experiment ................................................................................................. 19
Overview
.......................................................................................................................................... 19
Creating a Regime ............................................................................................................................ 19
Setting up an Experiment ................................................................................................................. 20
Filling the System to Eliminate Air Bubbles .................................................................................. 21
Post-Experimental
Analysis
............................................................................................................. 21
Application Notes ........................................................................................................... 22
Culturing Cells on Culture Slips
®
.................................................................................................... 22
Appendix: Parallel Streamer
®
Shear Stress Numbers ................................................... 23
Warranty Information ..................................................................................................... 24
Contacting Flexcell ......................................................................................................... 25
