F
LEXCELL
®
I
NTERNATIONAL
C
ORPORATION
5
All six slots must be filled to ensure proper
flow rate readings
.
If you do not wish to
use all six Culture Slips
®
with cells, use
blank Culture Slips
®
for the remaining
slots.
20.
Close the top of the device, turn the bolts by
hand, then tighten them with the hex
wrench provided with the system.
21.
Move the tray with the system components
back to the incubator. Put the Phar-Med
®
tubing back into the MasterFlex
®
pump
head and clamp the head down.
22.
Unscrew the small clamp on the Phar-Med
®
tubing to open the flow path to full capacity.
23.
Click the
Start
button in the software (or set
the pump to the desired flowrate and
depress the start button). Your regimen will
start and a green light will go on at the top
right corner of the screen.
24.
The expected shear stress and actual value
will be displayed on the graph in real time.
Periodically monitor the flow system for
leaks during the protocol.
25.
When the flow regimen is over and the
pump has stopped, remove the Streamer
®
system from the incubator as before. Open
the top and remove the slides for
processing.
26.
Clean the Streamer
®
and system with
deionized water.
Never leave culture media
in the Streamer
®
device after an
experiment, as this will corrode the
stainless steel finish over time.
See the instructional video,
Streamer®
Assembly
, on Flexcell
®
’s website
(http://www.flexcellint.com/videos-
instruct.htm) for a demonstration of how to
assemble the Flexcell
®
Streamer
®
and
associated tubing to run with a MasterFlex
Peristaltic Pump.
2. STREAMSOFT
™
V4.2 SOFTWARE
I
NSTALLATION
I
NSTRUCTIONS
1.
Insert the
StreamSoft
™
V4.2
DVD into the
DVD-ROM drive on the computer.
2.
Double click
My Computer
(Windows
XP) or
Computer
(Windows
Vista/Windows 7).
3.
Double click the
DVD-ROM drive.
4.
Double click the
Setup
installer.
5.
The installer will now open and run.
6.
On the
Product Notification
screen, click
Next.
7.
On the
Destination Directory
screen, click
Next.
8.
On the
License Agreement
screen, click
I
accept the License Agreement
and then
click
Next.
9.
On the next
License Agreement
screen,
click
I accept the above 2 License
Agreement(s)
and then click
Next.
10.
On the
Start Installation
screen, click
Next.
11.
Installation of the required National
Instruments and StreamSoft
™
software
will now begin.
12.
Once the installation is complete click
Finish
and restart the computer.
13.
Installation of StreamSoft
™
V4.2 is now
complete.
NOTE:
When the
Select Pump to Use
window
appears when opening the StreamSoft
™
V4.2
software, select the pump named
MasterFlex
Peristaltic Pump
to ensure correct function of the
equipment.
S
ETTING UP
P
ARAMETERS IN
S
TREAM
S
OFT
™
V4.2
Specific parameters will need to be set up in
StreamSoft
™
V4.2 to customize it for your
particular device and system.
Setting up
these parameters is extremely important to
