Expedeon Gelfree 8100 User Manual Download Page 27

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GELFREE 8100 Fractionation System

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27 of 30

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Bibliography

Tran, J. C., Doucette,

Entrapment Electrophore

A. A. (2008). Gel-Eluted Liquid Fraction

sis: An Electrophoretic Method for Broad

Molecular Weight Range Proteome Separation. Analytical

Chemistry,

, 1568-1573.

Summary of Contents for Gelfree 8100

Page 1: ...GELFREE 8100 Fractionation System Molecular weight fractionation with liquid phase recovery User Manual...

Page 2: ...ction of Parts 6 Instrument Placement 6 Description of Parts 7 GELFREE 8100 Fractionation Station 7 GELFREE 8100 Cartridge Kit 9 Operating Procedures 10 Installation and Power Up 10 Loading the Cartri...

Page 3: ...able power supply that accurately applies electrical potential stopping at user specified intervals for fraction collection A touch screen graphical user interface provides easy and simple navigation...

Page 4: ...mperature Limits Storage 10 60 C 30 80 relative humidity Operating 22 C 30 80 relative humidity Ingress Prot Rating IPXo GELFREE 8100 Cartridge Specifications Cartridge Size 12 5 cm x 8 5cm Gel Size L...

Page 5: ...irs and services should be performed by Protein Discovery Note The GELFREE 8100 Fractionation Station is capable of supplying up to 300 V per channel However the voltage recommended for best results i...

Page 6: ...tify Customer Service immediately Instrument Placement The GELFREE 8100 Fractionation Station includes a high voltage power supply and electronics that are sensitive to moisture extreme temperature an...

Page 7: ...ly for use with GELFREE 8100 Cartridge Kits It features a touch screen display power supply capable of delivering constant oltage 20 300 V fo retractable electrode a eferred to in this manu Figure 2 G...

Page 8: ...GELFREE 8100 Fractionation System 8 of 30 Description of Parts cont Figure 3 GELFREE 8100 Fractionation Station Electrode Array Cartridge Positioning Key 2013 Expedeon Inc All Rights Reserved...

Page 9: ...ch Cartridge Kit is the Running Buffer 500 mL and 5x Sample Buffer 1 9 mL which comprises all the buffer necessary to run all eight channels in the cartridge Note DO NOT REFRIGERATE d cast gels ensuri...

Page 10: ...lacement Opening the access to the c partment for loading and unloading the cartridg cting fractions during the experiment The cartridge is loaded such that the well numbers imprinted on the cartridge...

Page 11: ...Figure 5 GELFREE 8100 Fractionation Station with cartridg and retracted electrodes Follow arrows to lower electrodes Figure 6 GELFREE 8100 Fractionation Station with cartridge and lowered electrodes...

Page 12: ...REE 8100 Fraction Method Execution Method Monitoring evice is powered up the central navigation and be displayed on the touch screen interface cludes the key features necessary to navigate ntrol softw...

Page 13: ...n used and press OK This will lead to the screen shown in Figure 10 Touch the step number to be modified N provided with the fractionation method To create a new method follow these steps On the Main...

Page 14: ...ply Save Retrieve 1 85 V 05 00 2 85 V 11 00 3 85 V 01 00 5 6 7 8 9 85 V 05 00 10 85 V 05 00 4 85 V 01 00 85 V 02 00 85 V 02 00 85 V 02 00 85 V 02 00 Retrieve Method 1 99 1 Prev Next OK Cancel 2 1 3 5...

Page 15: ...elop Edit New Method Ste c Touch the inactive cell to activate it Then repeat steps a and b d The Next and Previous buttons allow the user to edit the next step or the previous step respectively A max...

Page 16: ...ference Card provided with each Cartridge Kit For a custom method the first step should be applied once the dye front is visible in the gel tube 16 minutes at 50 V 8 minutes at 100 V Executing a Metho...

Page 17: ...e polarity should be Negative thod has been applied and the channels selected press the Start button on the Main Screen to begin a run Once running the Start button becomes the Pause button which allo...

Page 18: ...is paused the circles will turn yellow If the method fails to start or fails during the run the circles will turn red When the circles are either yellow or red the voltage readout is zero Time Monitor...

Page 19: ...at is active is shown e g Step 1 of 12 50 V 16 min 5 Lid Status At the bottom right corner of the Main Screen a green circle indicates that the lid is closed ready safe to operate and a red circle ind...

Page 20: ...ple Buffer 5X c 8 L 1M DTT optional not provided in the kit d x L H ing the Cartridge uction Int It is perative that the steps below are followed in sequence Prepare the sample s and create or modify...

Page 21: ...trid temperature will caus best results run the Preparing the Cartridge a 1 Remove the cart plate sealer If all 8 channels in the cartridge will be utilized in one experiment dump out the storage buff...

Page 22: ...d the sample in the before running the cartridge rming the Run and Collecting the Fractions Place the cartridge in the instrument lower the electrodes and close the lid Start the method by touching St...

Page 23: ...d a band of dye will be visible near the left end of the gel Note Upon resuming the method check for proper operation as in Step 2 a 4 The Station will pause for collection of fraction second pre defi...

Page 24: ...ument and resume the 9 b Fill each Anode Buffer Reservoir with 8 mL Running Buffer c Fill ea Buffer d Fill the Sample Loading Chambers with 150 L Running Buffer e Fill the Sample Collection Chambers w...

Page 25: ...Inc for any replacement parts for E 8100 Fractionation System The use of parts buffer ridges that are not supplied by Protein Discovery use damage to the unit and cause the warranty to and void e Expe...

Page 26: ...ose specifications This warranty does not e ects resulting from actions of the user such as misuse o f r wiring operation outside of specification improper c or repair or unauthorized modification No...

Page 27: ...ibliography Tran J C Doucette Entrapment Electrophore A A 2008 Gel Eluted Liquid Fraction sis An Electrophoretic Method for Broad Molecular Weight Range Proteome Separation Analytical Chemistry 80 156...

Page 28: ...ed to sell or otherwise transfer the Product s the Product s components and or derivatives or materials made using or incorporating the Product s to a third party Expedeon will not assert a claim agai...

Page 29: ...ercial purpose e use in foods drugs devices or cosmetics of any kind or for consumption by or use in connection with or administration or application to humans or animals f resale of the Product s whe...

Page 30: ...8100 Fractionation System 30 of 30 Corporate Headquarters www Expedeon com...

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