GELFREE 8100 Fractionation System
(
20 of 30
)
Operating Procedures (cont.)
Runn
rod
im
1.
4.
! Impor
e p
deterg
rac
desalt
nd remove any known con aminants. If your sample has
particulates, we highly recommend passing the sample
through a 0.8 µm syringe filter to remove any loose
particulates prior to further preparation.
Once the sample is ready for preparation, please follow these
steps:
1.
In a 500 µL vial, combine the following:
a. Up to 112 µL Sample
b. + 30 µL Sample Buffer (5X)
c. + 8 µL 1M DTT (optional, not provided in the kit)
d. + x µL H
ing the Cartridge
uction
Int
It is
perative that the steps below are followed in sequence:
Prepare the sample(s) and create or modify existing method.
.
Prepare the cartridge and load the sample(s).
3.
Start the run.
Collect the fractions.
Preparing the Sam
2
ple(s)
tant Notice
Th
resence of low molecular weight contaminants, such as
ents, urea, and excessive salts will negatively impact the
tionation results. Before you start, be sure to fully
f
a
t
2
O
e. = 150 µL
Note: If the sample volume is less than 112 µL, add 18M
Ω
H
2
O to bring the final volume to 150 µL.
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