GELFREE 8100 Fractionation System
(
16 of 30
)
Operating Procedures (cont.)
5.
Review the voltage/time profiles for each step in the new
method. Press ‘Save’ on the Method Screen (Figure 10). A
pop-up box will appear with a default method number of 1.
Clear or type over the method number to give the newly
rea e
c
t d method a different number.
Nex , press ‘Apply’ on the same screen to go back to main
6.
t
ee
To edit
e
followin
in step
as de c ed
! Impo n
It is critical that ev
th the
GELFREE 8100 Fractionation Station incorporate a first step to
allow the user to add an additional 2 mL of Running Buffer to
the Cathode Buffer Reservoir (bringing the total volume to 8
mL) and to thoroughly rinse the Sample Loading Chamber.
For standard methods, the first step parameters are shown on
the Quick Reference Card provided with each Cartridge Kit.
For a custom method, the first step should be applied once the
dye front is visible in the gel tube (16 minutes at 50 V, 8
minutes at 100 V).
Executing a Method:
1.
Retrieve a defined method by pressing the ‘Method’ button
on the Main Screen as previously described. Once the
method has been retrieved, press ‘Apply’ to apply the
method and return to the Main Screen.
nnel’ button on the Main Screen. This will bring up the
Channel Screen (Figure 12). Select the combination of
channels to run during the experiment by pressing the
scr n (Figure 7).
an xisting method, follow the steps outlined above, with the
exception. Rath
g
er than using a number for a new method
3, enter the number of an existing method. Edit the method
above and then save the method.
t
s rib
rta
ery method developed for use wi
2.
Select the channels to run in the cartridge by pressing the
‘Cha
corresponding channel button (e.g. ‘Channel 1’), or press
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