GELFREE 8100 Fractionation System
(
21 of 30
)
Operating Procedures (cont.)
2.
Heat the vial for 10 minutes at 50°C.
Note: Overheating the sample will cause protein
ation.
ge, or room
e changes in elution time. For
system with all solutions at 22°C.
nd Loading the Sample
ridge from the foil package and remove the
de Buffer Reservoir
two 200
µL
aliquots
p
4.
ode
I
Tak
e
Cat
ill block electrical
current and impact elution time reproducibility. To dislodge
he
Buffer Reservoirs, simply flush the membrane with Running
Buffer using a pipette. Note: Overfilling the reservoir at this
point may result in irregular sample migration and separation.
degrad
3.
Allow sample to cool to room temperature (22°C).
Note: Changes in sample, buffer, cartrid
temperature will caus
best results, run the
Preparing the Cartridge a
1.
Remove the cart
plate sealer. If all 8 channels in the cartridge will be utilized
in one experiment, dump out the storage buffer found in all
compartments. If only some of the 8 channels will be utilized,
use a Pasteur or transfer pipette to remove only the Storage
Buffer from the reservoirs associated with the active
channels.
2.
Add 8.0 mL of Running Buffer to the Ano
for all active channels.
3.
After rinsing out the Storage Buffer with
of Running Buffer, add 150 µL of Running Buffer to the
ctive channels.
Add 6.0 mL of GELFREE Running Buffer to the Cath
Sam le Collection Chambe s
r for all a
e
Buff r Reservoirs for all active channels.
! mporta t Notice
e care to avoid introducing and trapping bubbles in th
hode and Buffer Reservoirs, as they w
n
bubbles potentially trapped against the membrane in t
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