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Using the Zernike phase contrast set.
Any Oxion model with a Zernike phase contrast set comes with the condensor and objectives
already mounted and centered on your microscope. If you suspect misalignment or want to check
the alignment please see the next point for ”centering the phase rings”.
The height of condenser can be adjusted in height by means of a rack and pinion movement. In
this way the light beam is concentrated in the specimen for an optimum resolution.
Centering the phase rings
Take following steps in order to centre the phase rings:
•
For centering of the 10x phase objective, turn the condenser disc in such a way that the
corresponding phase ring is in place under the condenser.
•
Place the centering telescope in the eyepiece tube, and focus the phase ring of the
objective by means of the adjustable eye lens.
•
Now focus the centering ring of the condenser by means of the coarse and fine adjustment
knobs.
•
At last, center the phase ring beneath the condenser disc with the phase contrast adjusting
levers, until the two rings visible in the eyepiece are in one centric line.
•
Repeat each step for all objectives.
Not centered
Centered properly
Using the Dark Field Slider
1.
Keep the dark field slider face up (text up); insert it from left to right into the condenser
slider socket as the direction of the arrow pointed.
2.
The slider has 2 positions, one position for dark field and one for normal bright field use
without dark field.
When observing in dark field, please fully open the diaphragm to maximum.
