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Electron Microscopy Sciences EMS 5000, Manual

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Electron Microscopy Sciences EMS 5000 Manual Download Page 9

A993 

OTS5000 

9

 

 
 

 

1.4.2 

ADDITIONAL ITEMS REQUIRED FOR OPERATION 

The following additional items are ORDERED SEPARATELY:

 

 

 

1 ea.  55-XXX Line Cord (Country specific see sec 2.1.4 of this manual for catalog number) 

 

 

 

1.4.3 

REPLACEMENT ITEMS 

 

 

Stainless Steel Slicer Blades (25ea.) 

                     EMS  

Mounting Blocks (1/2”) 

                     EMS  Mount

ing Blocks (1”) 

                     EMS  Specimen Vise 

                     EMS  Tray 

                     EMS Pedestal 

                     EMS Magnifier 

                     EMS Footswitch  

 

 

1.4.4 

OPTIONAL ACCESSORIES 

The following accessories are available: 

 

                                Glass Blade Holder 

                                EMS Pedestal Surface 

                                EMS Sapphire Blade 

 

 

1.5 

CONCEPTS 

 

1.5.1 

DESIGN DESCRIPTION

  

The EMS 5000 slices samples of fresh and fixed tissue, as well as plant and polymer material using an 
oscillating blade. An embedded microcontroller is used to digitally control functions such as blade 
position, advance speed, and automatic slice performance. Membrane switch technology in the manual 
control keypad and polymer packaging protects against corrosion from the laboratory environment. The 
blade arm is designed to eliminate unnecessary vibration to ensure slice integrity. Cold source focused 
lighting and a clear tray allow improved visibility. 

 

 

 

Summary of Contents for EMS 5000

Page 1: ...EMS 5000 Tissue Slicer ...

Page 2: ...des ________ 1 16 hex Driver ________ 55 AUS Australia ________ 55 CH China ________ 55 DAN Denmark ________ 55 EURO Europe ________ 55 ISR Israel ________ 55 ITA Italy ________ 55 JA Japan ________ 55 SAF South Africa ________ 55 SWI Switzerland ________ 55 UK United Kingdom ________ 55 USA North America ________ EMS Inc maintains a system of traceability to allow for product notifications in the...

Page 3: ...red For Operation 1 4 3 Replacement Items 1 4 4 Optional Accessories 1 5 Concepts 1 5 1 Design Description 1 6 Technical Summary 1 6 1 Specifications 1 6 2 Controls Connectors 1 6 3 Compatibilities 1 7 Illustrative Procedure 2 Reference Manual 2 1 Reference Information 2 1 1 Packaging 2 1 2 Mounting 2 1 3 Inspection 2 1 4 Power Connections 2 1 5 Warranty 2 1 6 Policies 2 1 7 Service 2 2 Installati...

Page 4: ...ement control and laboratory use Part 1 General Requirements EN50081 1 1992 Electromagnetic Compatibility Generic emission standard Part 1 Residential commercial and light industry EN292 1 1991 Safety of machinery Basic concepts general principles for design Part 1 Basic terminology methodology EN292 2 A1 1995 Safety of machinery Basic concepts general principles for design Part 2 Technical princi...

Page 5: ... necessary Storage Precautions Store at normal room temperatures between 34 C 29 F and 57 C 135 F Do not expose to temperatures below 39 C 29 F or greater than 70 C 158 F or a relative humidity of less than 10 or more than 100 including condensation or an atmospheric pressure less than 500hPa or greater than 1060hPa for long term storage Sterilization Except for the pedestal and mounting blocks th...

Page 6: ...try from solutions reduces handedness and improves ergonomics Slice thickness up to 999 microns set in 1 micron increments Focused LED s mounted to blade arm for constant direct cold source illumination Two specimen mounting options XY adjustable pedestal or vise block arrangement Removable easy to clean tray Accepts Razor Glass Diamond and Sapphire blades Precise bar graph displays of blade oscil...

Page 7: ...d LED s are installed in the blade head which provide constant cold illumination of the specimen during the slicing process Two specimen mounting options are available to the user a pedestal with forward backward and side to side tilting adjustments and a vise and mounting block 2 sizes provided assembly The blocks are used to quickly interchange multiple specimens on individual blocks The front p...

Page 8: ...INVENTORY 1 4 1 ITEMS DESCRIBED IN THIS MANUAL 1 ea EMS 5000 Includes EMS 5000 Footswitch Tray Magnifier Pedestal Specimen Vise Mounting Blocks 2 sizes Stainless Steel Slicer Blades 25 ea 1 16 Hex Driver Manual Not Shown ...

Page 9: ...lass Blade Holder EMS Pedestal Surface EMS Sapphire Blade 1 5 CONCEPTS 1 5 1 DESIGN DESCRIPTION The EMS 5000 slices samples of fresh and fixed tissue as well as plant and polymer material using an oscillating blade An embedded microcontroller is used to digitally control functions such as blade position advance speed and automatic slice performance Membrane switch technology in the manual control ...

Page 10: ...er 4 diameter X2 lens mounted on 12 30cm gooseneck 9 working distance Lighting Focused LED s mounted on the blade arm Dimensions Slicer 27cm x 40cm x 21 5cm 10 5 x 16 x 7 5 Keypad 17cm x 8 5cm x 3 4cm 6 7 x 3 4 x 1 35 Weight 13kg 30lbs Working space requirements 12 w x 18 d x 10 h 30 x 45 x 25cm Power requirements 85 265VAC 50 60 Hz 500mA max universal power input See Section 2 1 4 for instruction...

Page 11: ...cillation Displays the speed of the blade oscillation in bar graph form Each line of the graph represents a division between 0 and 5000 cycles per minute A blinking bar indicates that the setting is half way to the next bar Advance Displays the speed of the blade advance in bar graph form Each line of the graph represents a division between 0 and 2 5 mm per second A blinking bar indicates that the...

Page 12: ...rm down Momentarily pressing the button moves the blade arm the set thickness amount Pressing the button for longer than 1 second moves the blade at the fastest travel speed Speed Oscillation Pressing these buttons increments and decrements the blade oscillation speed Advance Pressing these buttons increments and decrements the blade arm advance speed Thickness Thickness Pressing these buttons adj...

Page 13: ...to begin slicing in multiple slice mode Pressing this button once will cause the blade arm to move forward and oscillate It will continue until it reaches a forward limit travel window or mechanical stop return to the reverse limit step down the selected thickness and begin another slice Pressing the button during this process will cause the arm and oscillation to stop and wait for a command The m...

Page 14: ...tissue with an eyedropper of cooled medium during the trimming process is suggested 8 Turn the side of the tissue to be bonded up and blot as dry as possible Apply a thin layer of tissue adhesive to the pedestal or block surface and immediately position the dry side of the sample on it ensuring that a strong bond is made 9 Position the pedestal in the tray Orient the pedestal angle if desired Alte...

Page 15: ... travel window Reverse limit 18 Reset the Oscillation and Advance speeds if desired to begin performing slices of the desired thickness in either Manual or Multiple Slice mode 19 Perform slices by either manually performing all blade arm movements using the keypad or footswitch and keypad combination or using the START STOP button to begin slicing within the set Travel Window 20 When slicing and h...

Page 16: ...lly checked against the inventory list sec 1 3 to determine shortages or errors in inventory 2 1 4 POWER CONNECTIONS The EMS 5000 is powered through an internal 12V power supply input 85 265VAC 50 60Hz 500mA max An international pattern Line Cord not shown is ordered separately and is specified by country per the catalog number See table below for catalog numbers The power entry uses double pole s...

Page 17: ...ating to your particular situation 2 1 7 SERVICE Should service be required please contact our Service Department for a return authorization number and instructions 215 646 1566 Please have the serial number on hand located on the back panel Carefully pack the instrument before returning Please include a note indicating 1 The model number and purchase date of the instrument 2 The person to contact...

Page 18: ...sure that the display increments by one with each press of the button The first press would cause the display to show the current thickness if it was previously showing the slice count Hold the THICKNESS button down Ensure that after one second the display advances rapidly 11 Press the THICKNESS button repeatedly Ensure that the display decrements by one with each press of the button The first pre...

Page 19: ...and ensure that the unit cycles in the new set window Press again to stop the cycle 29 Press the HOME button to return the unit to its full up and back position Slice Test Below is a test to determine integrity of the blade arm movement It can also be used to determine the correct oscillation and advance speed settings without using specimen tissue 1 Cut a block of hard boiled egg white approx 1 5...

Page 20: ... lift the pedestal straight up Alternately remove the vise in the same manner if using the vise mounting block arrangement 6 Prepare a volume of Ringers or other medium oxygenated with 95 O Put a small amount in a beaker immersed in an ice bath 7 Secure a new blade in the blade holder and tighten securely Cleaning the blade with acetone or alcohol is suggested to remove any grease or oil 8 To set ...

Page 21: ...e arm down at the chosen thickness setting 22 It may be necessary to perform multiple slices to initially prepare the sample Once a consistent slice is achieved usable sample slices can be performed by one of the following slice modes Manual Slice Mode 1 Position the blade at a convenient spot behind the slice 1 Press the ZERO button to set the slice counter to zero if desired 2 Set the slice THIC...

Page 22: ...or a command 5 Press the FORWARD LIMIT button if not previously set during initial setup During operation the blade will automatically return to the reverse limit from this point 6 To change the Forward or Reverse limits at any time during operation press the START STOP button once to stop movement Reposition the blade arm using the appropriate keypad buttons Repeat steps 2 5 to set the new slice ...

Page 23: ...n the back panel Indicated by the l side of the rocker switch pressed in 4 Press the START STOP button to activate multiple slice mode The foot pedal needs to be in this mode to function correctly 5 The foot pedal is used in place of the FORWARD keypad button in multiple slice mode above 6 Pressing the foot pedal down begins blade arm advance and oscillation Lifting off stops all movement 2 5 SCHE...

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