A993
OTS5000
21
12. Position the pedestal in the tray and set the sample angle if desired. To set the sample angle;
use the pivot pins on the side (y axis) and front (x axis) to set the angle, then secure the
pedestal by tightening the thumbscrew. Alternately, secure the mounting block in the vise,
and position the setup in the tray.
13. Immediately fill the tray with cooled medium. Ensure that the sample is covered by one or two
millimeters.
14. Slide the tray onto the mount and tighten the two thumbscrews to secure.
15. Set the ADVANCE speed to a high setting (15-20) using the appropriate keypad buttons.
16. Using the BLADE POSITION buttons, position the blade to an initial slicing position just
behind and above the sample.
17. Set the OSCILLATION and ADVANCE speed using the appropriate keypad buttons.
18. Set the slice thickness for the initial slice using the appropriate keypad buttons. In order to
initially prepare the sample, it is suggested that the first slices are relatively thick (50 microns
and up).
19. Press the FORWARD button once to initiate slicing, Once the blade has cleared the sample,
press the FORWARD button again to stop.
20. Press the REVERSE button to reposition the blade behind the slice. When the blade is in a
convenient position, press the REVERSE button again to stop movement.
21. Press the DOWN button once to move the blade arm down at the chosen thickness setting.
22. It may be necessary to perform multiple slices to initially prepare the sample. Once a
consistent slice is achieved, usable sample slices can be performed by one of the following
slice modes.
Manual Slice Mode:
1. Position the blade at a convenient spot behind the slice.
1. Press the ZERO button to set the slice counter to zero if desired.
2. Set the slice THICKNESS using the appropriate keypad buttons.
3. Set the OSCILLATION and ADVANCE speeds to an appropriate setting. Due to differences
in blade and sample type, these settings will be obtained empirically. Generally, the thinner
the slice, the slower the speeds.
4. Press the FORWARD button once to begin slicing.
5. Once the blade has cleared the sample, press the FORWARD button once to stop
movement. If allowed to continue, the blade will advance to its forward mechanical limit then
stop and wait for a command.
6. Press the REVERSE button once to move the blade back. Harvesting the slice can be
performed if desired.
7. Once the blade has cleared the slice, press the REVERSE button once to stop movement. If
allowed to continue, the blade will return to its reverse mechanical limit, then stop and wait for
a command.
8. Press the DOWN button once to lower the blade arm the set thickness amount.
9. Repeat steps 4
– 8 to repeat slicing.
10. To return the blade arm to its furthest up and back position, press the appropriate BLADE
POSITION button to stop movement if in motion (FORWARD if blade arm is advancing,
REVERSE if returning) then press the HOME button.
Summary of Contents for EMS 5000
Page 1: ...EMS 5000 Tissue Slicer ...
