DNAmaster Quick Manual
Version 1.0
Page
25
Quantification of Nucleic Acids
For the different types of nucleic acid solutions the average dynamic range of the absorbance relating to the
concentration (ng/μl) results and its required sample volume as follows (depending on the lightpath):
Factor
2 mm Lid
(Factor 5)
[ng/μl]
1 mm Lid
(Factor 10)
[ng/μl]
0.2 mm Lid
(Factor 50)
[ng/μl]
0.1 mm Lid
(Factor 100)
[ng/μl]
Total Detection
Range [ng/μl]
dsDNA
50
6 - 425
13 - 850
63 - 4250
125 - 8500
6 - 8500
ssDNA
37
5 - 315
9 - 629
46 - 3145
93 - 6290
5 - 6290
RNA
40
5 - 340
10 - 680
50 - 3400
100 - 6800
5 - 6800
Oligo
33
4 - 281
8 - 561
41 - 2805
83 - 5610
4 - 5610
Corresponding
Absorbance Value Limits
for A 260 (Abs)
0.025 to 1.7 0.025 to 1.7 0.025 to 1.7 0.025 to 1.7
Required Sample Volume 6 - 10 μl
3 - 5 μl
0.7 to 4 μl
0.5
to 3 μl
* 1mm and 0.2mm lid come as standard with DYNAM-UM model, for other pathlength’s lid, please enquire.