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Dynamica HALO DNAmaster, Operation Manual, Page: 21 / 44

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Dynamica HALO DNAmaster Operation Manual Download Page 21

DNAmaster Quick Manual 

 

Version 1.0   

   

 

 

 

 

 

 

 

 

Page

 5 

 

 

Dye incorporation   

Select dye incorporation method from 

dsDNA, ssDNA, RNA, Oligonucleotides 

Press   

1.

 

SET for detail setting 

2.

 

LOAD for reload saved parameter 

3.

 

ZERO for blank before measurement 

4.

 

READ for measurement 

5.

 

PRN for result printing 

 

 

 

 

 

 

 

 

SET 

Parameter setting 

Input 

1. Factor   

2. background correction (on/off) 

3. Pathlength 

4. Dilution Factor 

5. Unit 

6. Sample Name 

Press OK to next step or Save to store the 

parameters 

 

 

 

 

 

Select between 

different sample 

number 

Summary of Contents for HALO DNAmaster

Page 1: ...Model HALO DNAmaster Bio spectrophotometer HALO DNAmaster Bio spectrophotometer Operation Manual Revision D01 201203 ...

Page 2: ...ng measures Increase separation between the instrument and radio TV receiver Connect the instrument to an outlet on a circuit different from that to which the radio TV receiver is connected 2 Possible Electromagnetic Wave Interference Affecting This Instrument If this instrument is used near an intense electromagnetic source interference noise may be given to the instrument to incur an adverse eff...

Page 3: ...rate according to the specifications given in the instruction manual provided it is used in accordance with the instructions described in the manual 1 Scope of Warranty a Any parts which prove to be defective in design or workmanship during the warranty period will be repaired without charge b A substitute part may be used for repair or replacement with an equivalent product may be made instead of...

Page 4: ... to use of hardware software or spare parts other than supplied by us f Failure due to improper handling or maintenance by the user g Failure due to maintenance or repair by a service agent not approved or authorized by us h After disposal of this instrument or after its resale without our approval i Failure due to relocation or transport after initial installation j Failure due to disassembly mod...

Page 5: ...om this warranty Any other implied warranties of merchantability and fitness for a particular purpose are not included in this warranty No liability is assumed for direct or indirect damages arising out of explicit or implied warranties b Oral or written information or advice given by our dealers distributors agents or employees without our express permission shall not create a warranty or in any ...

Page 6: ...ed and discarded as instructed by the respective suppliers 2 Notice on Instruction Manuals 1 Information contained in the instruction manuals furnished with the instrument is subjected to change without notice for product improvement 2 This manual is copyrighted by Dynamica with all rights reserved 3 No part of this manual may be reproduced or transmitted in any form or by any means without our ex...

Page 7: ...t The hazard warnings which appear on the warning labels on the product or in the manual have one of the following alert headings consisting of an alert symbol and a signal word DANGER WARNING or CAUTION DANGER Indicates an imminently hazardous situation that if not avoided will result in death or serious injury It does not apply to this equipment WARNING Indicates a potentially hazardous situatio...

Page 8: ...ly related to the safety of a person Attention It is used to present warnings which are not directly related to personal injury hazards It is used to indicate prevention against damage to the equipment Note This is used to indicate instructions that enable you to operate the equipment accurately and perform accurate measurements ...

Page 9: ... you do not follow the instructions in this manual an inaccurate analysis may result or bodily injury may occur Because of danger please make sure not to modify or alter the product make sure not to use unspecified parts and make sure not to operate the equipment by removing defeating the safety device s When using chemicals please make sure to ventilate the room well If there is not enough ventil...

Page 10: ...installer Installation of this instrument shall be carried out by or under supervisions of qualified service personnel of Dynamica or its authorized service agent When relocation of this instrument becomes necessary after initial installation delivery please notify your local Dynamica sales representative or nearest Dynamica service office Technical support service for this instrument is available...

Page 11: ...armful to your health WARNING Eye Injury from Organic Solvents Handling Organic Solvents Please wear protective glasses when using organic solvents If the organic solvent should get into your eye flush your eye immediately under running water for at least 5 minutes while keeping your eyelids open See a physician for appropriate treatment ...

Page 12: ...ed WARNING Electrical Shock Electrical Shock from Contact High voltage is used inside of the equipment Never remove the cover except by a service person or as instructed in the section for lamp replacement When cleaning the inside of the cell compartment be sure to turn off the unit CAUTION High Temperature Burns from high temperatures The lamp will become very hot during operation please make sur...

Page 13: ... hand or hair caught Please assure your safety before operation CAUTION Fatigue due to Prolonged Work Data Processing Viewing the display in your work can cause eye and physical fatigue if you continue to work in the same posture for extended periods When working with the display for a prolonged period for your health make sure to take breaks for 10 to 15 minutes every hour in order to rest your e...

Page 14: ... NOTE Precautions regarding Accuracy and Precision of Measurements Do a routine check prior to taking measurements In order to obtain accurate measuring results please perform tests as instructed following the instructions in Chapter 3 ...

Page 15: ...ve grounding may not only cause lower resistance against noise from the outside but it can also cause the Mass Spectrometer and personal computer to generate static electricity which may involve the danger of electrical shocks A high voltage circuit is used inside the Spectrophotometer Do not open the covers when this circuit is operational because of the danger from electrical shock Fire Extingui...

Page 16: ...paint such as from organic solvents particularly benzene thinner etc 5 Other general notes a Avoid direct sunlight otherwise optical performance might deteriorate or the housing might become discolored Avoid installation by a window if at all possible b Vibrations or shocks strong enough to be felt by the human body must not be transmitted to the instrument otherwise the fine adjustment mechanism ...

Page 17: ...Operating Read button The Read button on the unit can use as an alternative way to take the data during measurement Touch screen DNAmaster s LCD display is pressure sensing which can be used even with gloves on For best performance fingertip or stylus is preferable Touch screen display Take Measurement on off button ...

Page 18: ...hotometric Repeatability 0 005 OD Stray light 0 5 T DNA detection limit 20ng µl Minimum sample volume with Ultramicro Cell Depends on the cap used 0 5µl Start up melodies Selectable from 7 types and mute Energy save mode Yes Memory storage Internal or SD card Data storage number 40 000 internal Unlimited external Manual program storage number 10 for each function Dimension W x D x H mm 300 x 300 x...

Page 19: ... go to next screen DNA RNA Nuclei acid analysis DNA RNA DYE Dye incorporation PROTEIN Protein determination PROTEIN UV Protein UV UV dye method OD600 Bacterial cell culture measurement SPECTRO Traditional spectrophotometer function FILE Saved method and data SETTINGS User parameter setting ...

Page 20: ... for blank or reference sample before measurement 4 READ for measurement 5 PRN for result printing Note Press ZERO key after new setting this will be used for all subsequent samples SET Parameter setting Input 1 Factor 2 320nm background correction on off 3 Pathlength 4 Dilution Factor 5 Unit 6 Sample Name Press OK to next step or Save to store the parameters Select between different sample number...

Page 21: ... detail setting 2 LOAD for reload saved parameter 3 ZERO for blank before measurement 4 READ for measurement 5 PRN for result printing SET Parameter setting Input 1 Factor 2 background correction on off 3 Pathlength 4 Dilution Factor 5 Unit 6 Sample Name Press OK to next step or Save to store the parameters Select between different sample number ...

Page 22: ...type Input 1 Dye Type For custom dye Input 1 Dye Abs Max for maximum absorbance wavelength of the custom dye 2 Dye Ext Coefficient for dye dependent extinction coefficient 3 Dye Correction for dye dependent correction factor at 280 nm Press OK to next step or Back for return to previous screen ...

Page 23: ...nce sample before measurement 4 READ for measurement 5 PRN for result printing Note Press ZERO key after new setting this will be used for all subsequent samples SET Parameter setting Input 1 Pathlength 2 Calibration mode Standard concentration or input K factor 3 Number of standards 4 Dilution factor 5 Unit selection 6 Sample Name Press NEXT for Standard concentration or K factor input OK to next...

Page 24: ...ration of standard used Press OK to next step or Back to previous screen For standard measurement For Standard curve setup Press 1 ZERO for blank before measurement 2 READ for measurement 3 PRN for result printing Note Press ZERO key after new setting this will be used for all subsequent samples ...

Page 25: ...eturn to previous screen PROTEIN SAMPLE MEASUREMENT Press 1 ZERO for blank or reference sample before measurement 2 READ for measurement 3 PRN for result printing Note Press ZERO key after new setting this will be used for all subsequent samples Change to standard curve display Select between different sample number ...

Page 26: ...ple before measurement 4 READ for measurement 5 PRN for result printing Note Press ZERO key after new setting this will be used for all subsequent samples SET Parameter setting Input 1 A280 Factor 2 A260 Factor 3 Pathlength 4 320nm background correction on off 5 Protein type 6 Dilution Factor 7 Unit 8 Sample Name Press OK to next step or Save to store the parameters Select between different sample...

Page 27: ...ample before measurement 4 READ for measurement 5 PRN for result printing Note Press ZERO key after new setting this will be used for all subsequent samples SET Parameter setting Input 1 A280 Factor 2 A260 Factor 3 Pathlength 4 320nm background correction on off 5 Protein type 6 Dilution Factor 7 Unit 8 Sample Name Press OK to next step or Save to store the parameters Select between different samp...

Page 28: ...return to previous screen Custom dye type selection For selected custom dye type Input 1 PRO EXT COEFFICIENT 2 Dye Abs Max for maximum absorbance wavelength of the custom dye 3 Dye Ext Coefficient for dye dependent extinction coefficient 4 Dye Correction for dye dependent correction factor at 280 nm Press OK to next step or Save to store the parameters ...

Page 29: ...e before measurement 4 READ for measurement 5 PRN for result printing Note Press ZERO key after new setting this will be used for all subsequent samples SET Parameter setting Input 1 Wavelength 2 Cell Pathlength 3 Unit 4 Sample name 5 Dilution Factor 6 Multiplier from 1000 or 1 000 000 Press OK to next step or Save to store the parameters Select between different sample number ...

Page 30: ...4 READ for measurement 5 PRN for result printing Note Press ZERO key after new setting this will be used for all subsequent samples SET Parameter setting Input 1 Data mode from OD Absorbance or T Transmittance 2 Pathlength 3 Sample Name 4 Select number of wavelength from 1 to 6 5 Wavelength nm need to measure Press OK to next step or Save to store the parameters Select between different sample num...

Page 31: ...D for measurement 5 PRN for result printing Note Press ZERO key after new setting this will be used for all subsequent samples SET Parameter setting Input 1 Data mode from OD Absorbance T Transmittance or E S Energy 2 Start wavelength 3 End wavelength 4 Y Axis maximum to be shown 5 Y Axis minimum to be shown 6 Sample name Press OK to next step or Save to store the parameters 540nm 200 ...

Page 32: ...w setting this will be used for all subsequent samples Data Process 1 P V Peak Valley function display the peak and valley 2 Smooth The smooth function employs a data smoothing algorithm to smooth the noise Selection of this function causes the spectral graph screen to change 3 Calculation K or K option performs addition multiplication of constant K on the spectral plot 4 Area for area calculation...

Page 33: ...nt 5 PRN for result printing Note Press ZERO key after new setting this will be used for all subsequent samples SET Parameter setting Input 1 Data mode from OD Absorbance T Transmittance or E S Energy 2 Scan wavelength nm 3 Scan time period 4 Pathlength of the cuvette 5 Y Axis maximum to be shown 6 Y Axis minimum to be shown 7 Sample name Press OK to next step or Save to store the parameters ...

Page 34: ...Note Press ZERO key after new setting this will be used for all subsequent samples Data Process 5 Kinetics Recalculate the kinetics by adjust the time period 6 Smooth The smooth function employs a data smoothing algorithm to smooth the noise 7 Calculation K or K option performs addition multiplication of constant K on the spectral plot 8 Area for area calculation ...

Page 35: ...ult printing Note Press ZERO key after new setting this will be used for all subsequent samples SET Parameter setting Input 1 Pathlength 2 Sample name 3 Select number of wavelength from 1 to 3 4 Wavelength nm need to measure 5 Unit 6 Calibration mode Standard concentration or input K factor 7 Number of standard Press NEXT for Standard concentration or K factor input OK to next step or Save to stor...

Page 36: ...ack to previous screen Concentration measurement After setting calibration curve Press 1 ZERO for blank or reference sample before measurement 2 READ for measurement 3 PRN for result printing Note Press ZERO key after new setting this will be used for all subsequent samples Change to standard curve display Select between different sample number ...

Page 37: ...DNAmaster Quick Manual Version 1 0 Page 21 File Data IN SD 1 Select between data stored in internal memory or SD card 2 Open Delete the method or data stored ...

Page 38: ...1 Home screen Select the function show on startup 2 Memory Storage Select storage by SD card or internal memory 3 Peak Threshold Select the threshold for automatic peak picking function 4 Factory reset reset back to factory default setting Time Time and Date setting ...

Page 39: ...nal service tools may required please contact your local distributor for further assistance User 1 User Name input 2 Company name input 3 Display contrast of the screen 4 Energy saving mode for screen turn off after 10 minutes without using 5 Start up tune 6 Tune volume 7 Key Tone on off when pressing ...

Page 40: ...ampling window and then back to the detector on the instrument It is supplied with a cap which contains a small mirror and it ensures that the sample is measured at constant pathlength The pathlength is achieved by a double pass through the sample as controlled by the cap height DNAmaster can setup the pathlength shown in the cap Just need to pipette a small drop 0 5 10μl depends on cap s pathleng...

Page 41: ...0 ng μl 0 2 mm Lid Factor 50 ng μl 0 1 mm Lid Factor 100 ng μl Total Detection Range ng μl dsDNA 50 6 425 13 850 63 4250 125 8500 6 8500 ssDNA 37 5 315 9 629 46 3145 93 6290 5 6290 RNA 40 5 340 10 680 50 3400 100 6800 5 6800 Oligo 33 4 281 8 561 41 2805 83 5610 4 5610 Corresponding Absorbance Value Limits for A 260 Abs 0 025 to 1 7 0 025 to 1 7 0 025 to 1 7 0 025 to 1 7 Required Sample Volume 6 10...

Page 42: ... temperature control function TM function For analyzing DNA melting temperature at 260nm select the OD600 TM function in the main menu Press 1 SET for detail setting 2 LOAD for reload saved parameter 3 ZERO for blank or reference sample before measurement 4 READ for measurement 5 PRN for result printing Note Press ZERO key after new setting this will be used for all subsequent samples ...

Page 43: ...then 2 min 5 Starting temperature minimum 20 6 End temperature maximum 95 7 Data resolution 8 Set EQUIL TIME for time lag after reaching start temperature before reading start 9 Set K factor i e GC content K Tm 69 3 x 2 44 Press OK to next step or Save to store the parameters SYSTEM The temperature control model can also used for sample incubation or temperature control In Setting menu input 1 Tem...

Page 44: ...ustralasia Dynamica Pty Ltd Unit C3 Hallmarc Business Park Corner Centre and Westall Roads Clayton Victoria 3168 Australia P 61 3 8540 5988 F 61 3 8540 5981 Email info dynamica com au Web www dynamica com au Asia Dynamica Asia Limited Room 607 Yen Sheng Centre 64 Hoi Yuen Road Kwun Tong Kowloon Hong Kong P 852 3583 1581 F 852 3583 1580 Email info dynamica asia com Web www dynamica asia com ...

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