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Diatron Abacus +, Service Manual

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Diatron Abacus + Service Manual Download Page 11

 

Abacus

+

 

Service Manual

 

 

rev 1.09 

11 

The  analyzer  performs  enhanced  Hemoglobin  measurement  technology  for  HGB 
measurement. The output of HGB head is frequency (TSL235 detector is light to frequency 
converter). This signal is counted by a digital counter in the FPGA circuit/micro-controller.  

This counter counts up while the LED is on and counts down while the LED is off, the LED 
and  the  counter  directions  are  switched  with  a  100  Hz  signal.  This  method  provides  “real 
time  backlight  correction”,  which  makes  the  HGB  measurement  more  precise  in  changing 
backlight environment situation as well. 

There are two kinds of HGB measurements: 

  Sample measurement 

(before RBC counting) 

  Diluent measurement 

(in WBC washing phase) 

The HGB result is calculated from these measurements by: 

HGB   log (CNT

diluent light

 / CNT

sample light

) 

In  spite  of  the  fact  that    is  less  sensitive  to  incident  light  changes  it  is 
recommended to keep side door closed during measurements. 

2.1.3.   Cell counter Amplifier Board 

Amplifier board includes its own voltage regulator, the connection interfaces to HGB head, to 
high  voltage  board  and  to  COMB  card.  In  this  board  there  is  the  current  generator  circuit, 
which works from 50 V measuring voltage (generated by High Voltage Board) and the probe 
voltage (DC) is amplified with a voltage follower (output: ELV). Nominal measuring current is 

870 µA

. 

Amplifier  board  includes  two  input  connectors  for  the  chambers  (measuring  electrodes). 
There  are  two  reed  relays  on  the  input  side:  IC10  can  select  between  the  two  channels 
(RBC, WBC) with RSW signal; IC11 connects high voltage to the selected probe with HSW 
signal.  Test  circuit  makes  possible  to  generate  test  pulses  (with  TEST  and  PLS  signals 
through FETs) for checking the proper operation of the amplifier channel.  

 

 

 

 

 

 

Connection to COMB (AMP and DIGIO)

 

Connection to the HVB

 

Reed relays

 

Summary of Contents for Abacus +

Page 1: ...ATRON Messtechnik Ges m B H A 1141 Wien Ameisgasse 49 51 2 AUSTRIA Tel 431 914 85 00 911 38 48 Fax 914 85 07 15 Web www diatron com DIATRON MI Ltd H 1038 Budapest Papírgyár u 58 59 HUNGARY Tel 361 436 98 00 Fax 361 436 98 00 Web www diatron com E mail support diatron com ...

Page 2: ...Diatron MI PLC rev 1 09 2 Revision history Revision Section Modification By At 1 00 All PRELIMINARY Support Team 10 11 2006 1 09 All Updating tubing schematic Cust Serv May 2007 ...

Page 3: ...DIC PARTS OF THE ANALYZER 19 2 2 1 Sampling needle 20 2 2 2 Washing head 20 2 2 3 H V moving unit 21 2 2 4 Main Dilutor 22 2 2 5 Micro Dilutor 23 2 2 6 Puffer reservoir 23 2 2 7 Pump 23 2 3 ASSEMBLED ANALYZER 24 2 3 1 Abacus 24 3 ADJUSTMENT 27 3 1 MECHANICAL SETTINGS 27 3 1 1 Opto wheel setting 27 3 1 2 Sampling needle setting 28 3 2 HARDWARE SETTINGS 28 3 2 1 Amplifier offset setting 28 4 OPERATI...

Page 4: ... 1 ERROR CODES 64 7 1 1 Software system errors 64 7 1 2 Pneumatic errors 64 7 1 3 Measure errors 65 7 1 4 Printing errors 66 7 1 5 Other errors 67 7 2 WARNING MESSAGES 68 7 2 1 Pneumatic warnings 68 7 2 2 Database relating warnings 69 7 2 3 Warnings relating to QC measure 69 7 2 4 Calibration relating warnings 69 7 2 5 Hardware relating warnings 70 7 2 6 Other warnings 70 7 3 POSSIBLE CAUSES OF NO...

Page 5: ...ackages Sent 83 10 2 3 Format of Acknowledge of the Receiver 83 10 2 4 Detailed Description of Packages 83 10 2 5 Serial Protocol 1 0 88 10 2 6 Serial Protocol 1 7 89 10 2 7 Serial Protocol 2 20 2 23 90 10 2 8 Serial Protocol 3 0 91 10 3 ABACUS CABLING DIAGRAM 95 10 4 ABACUS TUBING SCHEMATICS 96 10 5 RECOMMENDED KIT OF TOOLS 97 10 6 ELECTRONIC SCHEMATICS 97 ...

Page 6: ...Diatron MI PLC rev 1 09 6 ...

Page 7: ...ery important information for the Service Personnel about the service operations and possible problems 1 1 Name and serial number Name Abacus Hematology Analyzer Serial No Every instrument has its own serial number which is printed on the rear panel label and it can be read out from Device Information or from the self test submenu This identity number is write protected by DIATRON 1 2 Intended use...

Page 8: ...rsion number can be read out from the Device Information or from the Self test submenu Software is absolutely Plug and Play it can read out and detect the type and the serial number of the instrument therefore it will run the correct program for the hardware without any user or service help Every Abacus software version is upgradeable using an USB drive by the latest program developed by DIATRON a...

Page 9: ...alve pneumatic controller pump driver and power supply for internal printer 8V and digital circuitry 5V 6 Safe configuration E2 PROM board connecting CPU board and PPB 7 Motors with common opto board of needle moving motors H V 8 Main dilutor block with opto board for diluent lyse 9 Micro dilutor block with opto board for sampling 10 Valve boards set of 5 and max 7 11 Peristaltic Pump 12 Pressure ...

Page 10: ...e pulses In the instrument there are two Cellcounter Probes WBC probe with 100 μm aperture and RBC probe with 80 μm aperture Both have a ground electrode assembly and U shaped metal fixing as it is shown in the next figure The aperture is made of ruby and it is moulded into the measuring tube 2 1 2 HGB Head Hemoglobin head is placed on the two sides of the WBC chamber It contains light source LED ...

Page 11: ...ite of the fact that Abacus is less sensitive to incident light changes it is recommended to keep side door closed during measurements 2 1 3 Cell counter Amplifier Board Amplifier board includes its own voltage regulator the connection interfaces to HGB head to high voltage board and to COMB card In this board there is the current generator circuit which works from 50 V measuring voltage generated...

Page 12: ... micro controller DIMM PC itself contains 16 or 32Mbyte RAM and same size of FlashDisk that acts like a hard disk DIMM PC module is easily replaceable as it has an open socket it has also a screw for safe fixing COMB card contains single ICs and some drivers protection circuits for the interfaces such as COM1 PS2 USB IDE and Speaker Measurement processing is based on a FPGA circuit After power on ...

Page 13: ...odules GmbH 2 1 6 Configuration and ID E2 PROM board IDEPROM This board is the interconnection between COMB and PPB cards Pneumatic I 2 C bus power lines and internal printer signals are connected through this card The board also contains a 24FC256 serial E 2 PROM which stores the FPGA s configuration data and identity information of the instrument Serial Number OEM model etc Keeping the hardware ...

Page 14: ...d pneumatic system s parts Power system generates 5V Digital power 8V Printer power and 12V Motor and valve power from the single 12V DC input signal Motor driver part consists of six separated PIC micro controllers with power drivers Horizontal Vertical and Sample rotor motors have one combined ribbon cable connection Main Dilutor with two motors and Micro dilutor have separated connectors Valve ...

Page 15: ...s and have opto switches for the motor s home and end positions The actual status of the stepper motor s optos is indicated by two LEDs on each stepper motor opto boards Dilutor and Micro dilutor have its own separated opto board located directly in the units Horizontal and Vertical motors have a common Opto board called XYopto Board The other side of the board contains a ribbon cable connection t...

Page 16: ...n the pressure sensor 2 1 11 Digital Reagent Sensor Board This board contains four liquid detector opto detectors optos and a reference opto for automatic temperature and stray light compensation The reference opto is located in the middle and it has the same temperature and backlight conditions as the sensing ones The Reagent Sensor Board is connected to the PPB card and the valve driver micro co...

Page 17: ...equency circuit producing suitable voltage for CCFL cold cathode fluorescent lamp of the LCD The CFSW digital signal from the COMB card controls HVB logical LOW turns inverter on The MVON digital signal from the COMB card switches the measuring voltage 50 V on off by O1 opto switch Warning Be careful with servicing this board in active state because the high voltage 300V at LCD lamp connector can ...

Page 18: ...h an external power supply The next figure shows the power supply unit generating 12VDC The power supply modules have an auto range input which makes possible to use them with 230V or 115V mains outlet and it has the CE and UL safety certification The input socket of the power supply is a standard 3 terminal plug with power cable connection the output is a special lockable socket as it is shown in...

Page 19: ...dic parts of the Analyzer Abacus Hematology Analyzers consist of the following mechanic and fluidic parts 1 Sampling needle 2 Washing head 3 H V moving unit 4 Micro Dilutor 5 Dilutor 6 Chambers 7 Cell counter probes 8 Puffer reservoir 9 Pump 10 Valves 11 Tubing ...

Page 20: ...tments 2 2 2 Washing head Washing head is located at the bottom of the H V moving unit and it is for cleaning the outer surface of the sampling needle This washing process is made with diluent reagent and the fluid is drained by the pump The arrows on the picture show the direction of diluent flow during sampling needle washing Clean or replace washing head yearly or after 10 000 measurements Clea...

Page 21: ...These are required for correct initialization and error detection All sensors have status LEDs to show actual conditions The Vertical motor works with a special opto wheel for detecting home end positions See the Adjustment section of this manual to place this wheel to the proper position Greasing of the horizontal vertical guiding rods should be done regularly using Photolube A598 a PTFE based th...

Page 22: ...ogged end of the Macro and Lyse pistons between the syringe and the tip itself This will ensure optimum sealing and longer lifetime of piston tips Greasing of the cogged transmission parts cogwheel and cogged bar should be done regularly using machine grease It is recommended to check and repeat greasing of piston tips and transmission gear every year or after 10000 measurements Lyse syringe and p...

Page 23: ... adjusts measuring vacuum according to it 2 2 7 Pump Pump generates regulated vacuum and drains the fluidic system It is connected to the PPB and it has its own driver circuit Darlington If the tube of the peristaltic pump becomes worn it can be broken causing Pressure error It is recommended to check the state of the tube and replace it every 2 years or after 20 000 measurements Always replace th...

Page 24: ... 3 1 Abacus Front Panel Rear panel 240x128 dots Graphic LCD Built in thermal printer optional START button Function keys Serial Port USB slave port Warning labels USB ports Power switch 12V DC inlet Foil keypad PS 2 external keyboard port Reagent inlets ...

Page 25: ...uction front Construction right side Speaker Valve block H V unit Electronic block Valve block Pressure sensor Valve block Reagent sensor Puffer reservior Microdilutor Pump Dilutor block Valve block Measuring chambers Needle moving mechanics ...

Page 26: ...Diatron MI PLC rev 1 09 26 Construction left side Control and Measurement Board COMB Pneumatic and Power Board PPB ID Eprom Board IDEPROM Speaker ...

Page 27: ...or the vertical motor movements because this adjustment sets the opto end switches of the H V moving unit The top of this block is called HV head and it is shown in the figure below Set the distance to 1 2 mm between the moving carriage and the stable part of the head Loose A screws to allow free movement of the timing belt Adjust the opto wheel to home position i e home hole must be in home senso...

Page 28: ...the washing head s bottom plane while the carriage is held by motors Needle setting menu Fix the B screws Be careful with the bent upper end of the sampling needle because if badly aligned during movement it can hit other mechanical components causing mechanical jam and therefore damages or error 3 2 Hardware settings 3 2 1 Amplifier offset setting Amplifier offset should be between 5mV Run self t...

Page 29: ...ng process 1 8 WBC chamber draining process 1 9 RBC chamber draining process 1 10 Cleaner priming process 2 Main functions of the fluidic system 2 1 Initialization 2 2 Wake up 2 3 Measurement cycle 2 4 Standby 2 5 Cleaning 2 6 Hard cleaning 2 7 Shutdown In the detailed process description figures the active tube is filled with black or gray color while an arrow shows the direction of the flow Movi...

Page 30: ...syringe makes the aspirating while the M5 Micro dilutor motor moves down There is another sampling process for the second RBC dilution when 35 μl of primary dilution is aspirated from the MIX by the aspirating needle Both of the samples are separated from the diluent with a small air bubble and there is another air bubble between the sample and the end of the needle ...

Page 31: ...ards so that the total length of it is washed and cleaned This process is called total sampling needle washing and it is mainly used after taking primary sample from sample tube Another process which is washing only a smaller part of the sampling needle is the same but the needle does not move in the total length Some procedures perform this kind of sampling needle washing The M3 Macro syringe pus...

Page 32: ...ng needle In the first diluting step the sample is dispensed into the MIX chamber with 3 ml of diluent 1 0 ml is waiting in the MIX chamber which comes from the Macro syringe through V11 On and Micro dilutor while the M3 Dilutor motor moves upwards This process makes the 1 160 first dilution rate in the MIX chamber The second sampling process aspirates 35 μl of primary dilution and then it is adde...

Page 33: ...s step the liquid remaining in the mixing chamber flows via the V0 On into the WBC chamber because the vacuum which is in the puffer reservoir and maintenated by the pump aspirates the fluid through V5 On and V3 On while V2 and V1 are Off Simultaneously the peristaltic pump supports this procedure via V4 On ...

Page 34: ...ution remaining in the mixing chamber flows into the WBC chamber like a simple MIX WBC transfer but in this case simultaneously 0 8 ml of lysing reagent is added through V9 On while the Lyse syringe moves upwards This process makes the 1 192 dilution rate and good mixing with lyse ...

Page 35: ...s The regulated vacuum it is generated by the pump in the puffer reservoir aspirates the diluted sample WBC and RBC from the chamber through V2 On valve The instrument counts the cells for 5 seconds in both counting phases at first WBC and after it the RBC ...

Page 36: ...branch and backsides of the apertures are filled with diluent and After generating vacuum in the puffer reservoir the pump continues the drainig of the aperture branch V2 and V3 On V1 and V5 Off meanwhile the diluent syringe of M4 dilutor unit pushes diluent through V10 On V6 On and V8 On ...

Page 37: ...s The WBC chamber draining is performed via V3 On and V4 On valves by the pump while the V5 must be off The WBC chamber draining is always executed without using the puffer reservoir So other tasks like RBC chamber draining can be performed parallel with this operation ...

Page 38: ...rained by the vacuum in the puffer reservoir through V1 On valve V2 must be off This vacuum is made and maintenated by the peristaltic pump During the draining the pump can perform either other task like WBC chamber draining or needle wash or can help the draining of the RBC chamber ...

Page 39: ...de of the RBC aperture back side of the WBC aperture V2 On V3 On V4 On and the peristaltic pump The V1 and V5 valves are off The flow of the cleaner is controlled by the vacuum generated in the puffer reservoir before the start of the priming After the priming the remaining cleaner between the cleaner detector and V6 approx 1 ml can be dosed into the mix chamber ...

Page 40: ...ng 4 2 2 Wake up If the instrument is in standby state the wake up process is taken place in order to make the instrument ready to measure During this process the needle is go out and down to the sampling position and the M1 and M2 stepper motor hold it until the start button pressed or the instrument go into standby While the needle is moving the microdilutor M5 is aspirate some air into the need...

Page 41: ...id used The RBC chamber contains the last rinsing amount of diluent of the previous measurement cycle or the standby volume The following two flowcharts introduce the entire measurement cycle One of these flowcharts describes the fluidic transfers related the needle the other one the chamber related activities The flowcharts has synchronization points where one of them waiting W for the others sig...

Page 42: ...2 First dilution MIX chamber S3 RBC sampling Dilutior M3 moves down W3 Needle short washing S4 Dilutior M3 moves down Needle positioning over the RBC chamber W4 Second dilution RBC chamber S5 Needle up Dilutior M3 moves down W2 ...

Page 43: ... Service Manual rev 1 09 43 W5 Microdilutor syncronization Fill up the MIX chamber with 2 5ml of diluent S6 W6 Fill the MIX chamber with 2 5ml of diluent S7 Perform wake up process Dilutior M3 moves down END ...

Page 44: ... blank S1 Dilutor M4 moves down and primes diluent HGB blank delay W1 RBC chamber draining Fill up the MIX chamber with 1 ml of diluent Dilutor M4 moves down and primes diluent Fill up the RBC chamber with 1 ml of diluent WBC chamber draining W2 S2 S3 Lysing ...

Page 45: ...uent Aperture priming Generating measuring vacuum WBC counting HGB measurement RBC counting S4 RBC chamber draining WBC chamber draining Pull out 0 2 ml of Lyse Autoclean Fill up the RBC chamber with 0 8 ml of diluent Fill up the RBC chamber with 2 5 ml of diluent W4 No Yes ...

Page 46: ...leaner Fill up the RBC chamber with diluent Aperture Priming MIX WBC Dilutor M4 moves down and primes diluent High voltage cleaning pulses backflush RBC chamber draining WBC chamber draining Fill up the MIX chamber with 2 5 ml of diluent and cleaner S5 Dilutor M4 moves down and primes diluent W5 Fill up the RBC chamber with 4 5 ml of diluent ...

Page 47: ...uent is put into MIX via needle and the RBC chamber via V8 and the diluent from the MIX is transferred into the WBC chamber and at last MIX chamber is filled with 1 ml of diluent and the back side of the apertures are primed The diluent in the chambers prevent the apertures and chambers from dirt drying out and salt build up MIX WBC Autoclean Yes No High voltage cleaning pulses backflush Dilutor M...

Page 48: ... the standby state are dosed into the chambers 4 2 6 Hard cleaning Before the hard cleaning a wakeup procedure is executed After pressing the start button the M3 dilutor aspirates 0 2 ml from the hard cleaning solution Half of this amount is dosed into the MIX chamber diluted with 5 0ml diluent coming from the other dilutor unit M4 through V10 V8 V6 and the remaining cleaning solution is pushed in...

Page 49: ... generating 20000 test pulses incl gain related peak value noise related deviation Atmospheric pressure stands for outer pressure Vacuum reports pump operation vacuum made by the pump in a 10 second period of time Drift represents pressure loss of vacuum measured in a 10 second period of time Power 12V shows the amplifier voltage value Power Batt reports the voltage value of the battery If battery...

Page 50: ...lifier board Electrode voltage LOW or HIGH Fault on High Voltage or Amplifier board Check measuring voltage 50V on High voltage and Amplifier boards Current LOW or HIGH Fault on Amplifier board Check current generator and test generator FET on Amplifier board Offset LOW or HIGH Fault on Amplifier board Check the offset potentiometer on Amplifier board Amplifier test LOW Amplifier Boards is not con...

Page 51: ... Service menu 6484 5 2 2 Main Service Menu The Main Service menu provides access to submenus and service utilities 5 2 3 Edit service contact Here you can edit the Information card fields by cursor keys or by an external keyboard Press the OK button if a field is completed This information will appear in the User Service menu 5 2 4 Device Information In the Device Information menu the model name t...

Page 52: ... factors to 1 00 5 2 6 Settings 5 2 6 1 Pneumatic Measurement options Service Person can set the parameters of the automatic functions in this dialog menu Standby time after how many minutes the instrument goes to standby mode default 15 min Reblank time after how many minutes the instrument makes a blank measurement if it was in standby mode default 1 hour If it is set to 0 then no ReBlank is per...

Page 53: ...es to contain the header of the blood result Reagent pack Using packed reagent in Reagent status menu system displays the reagent pack installation day and the Reagent open stability period User can change reagents only together In case of No reagents can be changed separately the date of expiry is ignored Waste container capacity it essential to set the correct volume of the waste container for p...

Page 54: ...sing larger database the instrument software startup time can be increased Native keyboard Setting it to Yes will utilize the external PC keyboard with the language specific layout Calendar mode You can select between the Gregorian and the Jalaali Persian calendar All dates will be converted accordingly Patient data There are two options Birthdate and Age According to this setting both in the samp...

Page 55: ...r under each that represents the actual state of the valve P means peristaltic pump Selected valve number is shown in inverse 0 is Off state 1 is On state Use cursor keys for selecting and setting valve state or press OK to toggle state 5 2 9 Motor Test Menu Pressing numeric key on keyboard the regarding motors start operation repeatedly until pressing 1 or another key All motors stop at initial p...

Page 56: ...rms measuring cycles without sample blank measurements continuously This can be used for burn in tests or to check pneumatic system after changing any main fluidic parts You can have information about stability cleanliness HGB operation and counting time stability Results of the last 10 PLT and HGB blank is displayed as well You can detect any kind of noise or bubbles in the system if the PLT is n...

Page 57: ...ing this menu the needle will go to the position you can adjust it You can Log in as supervisor if the Multi user mode was selected in the User s Settings submenu Number 4 if present shows the availability of this function In supervisor mode you have the ability to change any user passwords and you have full access over user settings Always log out after this supervisor log in 5 2 13 Multi user Re...

Page 58: ...en After 6 seconds it starts to scan the keypad and behaves like an MDA adapter to the PC although the displayed screen is still holding the greeting screen If you switch to MDA emulation mode the system boot events can be displayed and the CMOS SETUP program can be started if necessary If the main program will not start in 90 seconds the PIC will automatically switch to MDA displaying mode to giv...

Page 59: ... drive Base I O address Primary Serial Port A Enabled Base I O address 3F8 Interrupt IRQ4 Serial Port B Enabled Base I O address 2F8 Interrupt IRQ3 Parallel Port Enabled Mode EPP Base I O address 378 Interrupt IRQ7 Watchdog Settings Mode Disabled I O Chip Select I O Base Disabled Halt on Errors No SECURITY Fixed disk boot sector Normal Virus check reminder Disabled System backup reminder Disabled ...

Page 60: ...0 I is equipped with a JUMPtec Embedded BIOS which is located in a Flash EPROM onboard This device has an 8bit wide access Faster access is provided by the shadow RAM feature default For a detailed description of the BIOS Setup please refer to the section below The Setup Guide With the PhoenixBIOS Setup program it is possible to modify BIOS settings and control the special features of the computer...

Page 61: ...rrow key to move the cursor to the field you want Then use the plus and minus keys to select a value for that field The Save Value commands in the Exit Menu save the values currently displayed in all the menus To display a sub menu use the arrow keys to move the cursor to the sub menu you want Then press Enter A pointer 4 marks all sub menus The Field Help Window The help window on the right side ...

Page 62: ...S DOS boot floppy into the floppy disk drive After turning on go to the BIOS setup press F2 repeatedly on the keyboard and switch to MDA mode by pressing i Change the Boot Device Priority in the BOOT menu The Removable Devices must be the first in the list Select Removable Devices and move it up by pressing on the keyboard Save the actual settings to CMOS the instrument will restart Exit Saving Ch...

Page 63: ...e LCD display if any kind of malfunction is detected The electronic parts have a very little chance to fail only the connections and cables could disconnect which can cause the malfunction of the electronic system The mechanic and fluidic system have a bit more chance to go wrong because it has moving parts This section allows to know what to do when a troubleshooting message appears on the screen...

Page 64: ...ower off the system Cannot make the measuring vacuum Check the pump and the tubes fittings and valves around the puffer reservoir 1101 Waste is full Empty waste container The system assumes that you will do it before going on Waste container is full Empty waste container 1102 Check Diluent container Diluent reagent con tainer become empty or one of the diluent fluid sensor is too sensitive or ther...

Page 65: ...id sensor is too sensitive or there are lot of bubbles in the cleaner tubes Replace the Cleaner reagent container with a filled one If this error still remains with a filled cleaner reagent container calibrate sensors and check the tubings and the fittings 1113 Unrecoverable pneumatical error occurred n Device s n Error code s n Status x n Please turn off the instrument and turn it on again Fatal ...

Page 66: ... the Settings Printer settings sub menu 1405 Paper height is too large Paper height steps over the margin Modify the paper setup in the Settings Printer settings sub menu 1406 Paper width is too small Paper width steps over the margin Modify the paper setup in the Settings Printer settings sub menu 1407 Paper height is too small Paper height steps over the margin Modify the paper setup in the Sett...

Page 67: ...r change to single user mode in the User settings submenu 1921 s sensor calibration failed The system turned off the s sensor Reagent container became empty or there are lot of bubbles in reagent tubes Replace the reagent container with a filled one If this is still a problem with a filled reagent container turn on the sensor and try recalibrating and check the tubings and the fittings ...

Page 68: ...ve waste connected After pressing the analyzer drains itself 5004 You can power off the system The preparing for shipment function is finished Power off the instrument 5005 Apertures are partially clogged Try cleaning During the measurement one or both of the apertures were clogged C for WBC c for RBC aperture Try cleaning perform blank measurement If the problem still persists use the hard cleani...

Page 69: ...ce into the USB port The analyzer will save u number of data records Insert USB storage media and press 7 2 3 Warnings relating to QC measure Code Message Reason s Remedy 5200 Your previous QC data will be lost Are you sure When you press for accepting new QC target values If you press the new values will be accepted and all of the measurements on the selected QC level will be deleted If you press...

Page 70: ...e Personnel 7 2 6 Other warnings Code Message Reason s Remedy 5901 Insert software update media The system will restart Software upgrade USB After pressing the system will restart 5904 This function will delete all selected records Delete If you press all records will be deleted If you press it will be not deleted 5906 Now it is safe to turn the instrument off Shut down process has been finished P...

Page 71: ... i e the background PLT blank becomes high Wash the reagent tube which is in connection with the reagent with 1 of bleach solution then rinse with clean distilled water or diluent It can avoid the bacteria to grow inside If tank is open and cap is not installed or closed external dust can make reagent dirty 7 3 2 Bad earth grounding In this case external ground referenced noise can get into the sy...

Page 72: ...metal box allows this coupling to disappear forever 7 3 4 Internal noise sources The most annoying but real cause is some sort of internal noise The reason for this phenomenon is that inside electrode hot point of the measuring circuit must be well insulated from surrounding electronics otherwise inside noise sources can take their effect 7 3 4 1 Bad chamber insulation bad shielding of the chamber...

Page 73: ...ectrode socket or replacement of amplifier is required Check for the correct soldering of reference cable and its connector circuit board bad soldering or component failure Check the shielded cable connections as well Sometimes inside out connection hot electrode goes outside as a shield is the problem both ends of amplifier signal cable must be reversed analog signal ribbon cable it picks up nois...

Page 74: ... Dilutor Piston Tips The cogged end of PTFE dilutor pistons should be cleaned and lubricated by neutral silicon grease A599 Apply just a thin layer and move it along the perimeter of the piston so that some of the material goes into the gaps between the sealing rings Repeat this step for lyse and dilutor pistons as well Check the condition of the micro piston sealing and replace if necessary 8 2 4...

Page 75: ...rdness of tubings at rear panel lyse connection by hand If you feel the Tygon tubing hard compared by other tubing replace them 8 2 8 Bleaching of Fluidic System It is recommended to run a bleaching procedure to remove stains from the fluidic system 1 Connect 2 5 hand warm clean bleach solution to all reagent inputs and perform priming on all reagent inputs 2 Leave it in the tubing for not more th...

Page 76: ...M board J112 Control board COMB 4 12 J113 Control board for DIMMPC A192 DIMMPC CPU A195 DIMMPC safe ring AP300 XY needle movement block complete A301 Timing belt for XY block A302 Stepper motor A303 Bearing for XY block A304 Timing pulley for X mounted A305 Timing pulley for Y mounted A306 Gear for Y opto AP307 H V motor opto board complete A308 Opto switch I A309 Opto switch II A310 Head washer A...

Page 77: ...r A434 HGB meas head complete J5P900 Microdilutor unit complete AP501 Valve unit I complete AP502 Valve unit II complete A504 2 2 valve A505 3 2 valve A506 Valve coil for both valves A507 2 2 valve head A508 3 2 valve head A510 Puffer reservoir Abacus A511 Puffer reservoir holder pair J530 Pressure sensor with cable complete AP240 Reagent sensor board A541 4 2 plastic tube silicon A542 5 3 plastic...

Page 78: ...r pump pair A565 Connector for pump pair A610 Micro switch APX105 Abacus cover with printer APX107 Complete start bar AP620 Instrument door AP621 Door lock AP640 Abacus sample union A650 Screw for loudspeaker A701 Mains cable J728 5V regulator with cable J7502 34P ribbon cable XY block J7503 10P ribbon cable printer C722 Amplifier shielded cable AP701 Ground cable 15 cm AP702 Ground cable 25 cm AP...

Page 79: ...bon cable microdilutor AP7507 26P ribbon cable dilutor AP7508 3P ribbon cable start button AP7509 10P ribbon cable HUR AP132 Foil keyboard AP140 Amplifier board complete AP250 Dilutor block complete AP251 Lyse syringe with piston AP202 Lyse syringe S210 Bearing for dilutor J214 Dilutor panel mounted ...

Page 80: ... warning Check the 1 RBC LYM discriminator If it is in the minimum point or close to it accept the results Otherwise repeat the measurement If the retried action makes very similar results and the discriminator is in a wrong place then the MID and GRA results are OK but the WBC and LYM results can be higher because of the RBCs R Too many RBC cut from WBC Repeat the measurement Possibly lyse proble...

Page 81: ...is rather a diagnostic flag If the discriminator is in a wrong place in the PLT or RBC histogram then repeat the measurement for a correct PLT result k RBC peak warning Perform cleaning and repeat the measurement clogging If it is a general problem change the RBC aperture m RBC PLT coincidence is too high Linearity error The same action as in case of the M warning flag d RBC PLT data package error...

Page 82: ...ry to communicate any more From this state it can wake up by receiving an ENQ ASCII code 5 character from the other side and Abacus will immediately respond with an ACK ASCII code 6 From this point Abacus will send data if it is ready for transmission The communication is based on packages There are 7 package types INIT package Device identification software version current date and time DATA pack...

Page 83: ... to a request for histograms If no more packages are required SPACE should be sent MID the identifier of the package that acknowledged by this message If receiving of the message was not successful NAK should be sent and Abacus will repeat the last package The receiver has approx 1 second to reply Otherwise Abacus will repeat the last transmission twice automatically But if still no response after...

Page 84: ... WM1 HT 23 LF WBC upper marker 2 WM2 HT 57 LF WBC LYM MID marker 2 WM3 HT 92 LF WBC MID GRA marker 2 PARN HT 22 LF number of parameters to send P01 HT 6 6 HT 0 LF first parameter ID 3 value and flag 4 P02 HT 4 29 HT 0 LF second parameter ID value and flag P22 HT 8 2 HT 0 LF last parameter ID value and flag Remarks 1 Position and meaning of the warning flags Bit Mask Letter Meaning 0 0x00001 c q RB...

Page 85: ...uld not be calculated because of an error The meaning of the parameter flags Flag Displayed Meaning 0 Value correct 1 Value high more than upper limit 2 Value low less than lower limit 3 Value is unreliable 4 E Value not given because of error Value is 5 Value cannot be calculated There is no value RBC WBC and PLT package The format of the histogram packages are the same they differ in type descri...

Page 86: ...ength 1 path Measures Measure field_PLTH alias PM1 Field name PLThi type BYTE length 1 path Measures Measure field_PLTH alias PM2 Field name RBClo type BYTE length 1 path Measures Measure field_RBCH alias RM1 Field name WBClo type BYTE length 1 path Measures Measure field_WBCH alias WM1 Field name LYMhi type BYTE length 1 path Measures Measure field_WBCH alias WM2 Field name GRAlo type BYTE length...

Page 87: ...A 16 define PAR_LYMp 17 define PAR_MONp 18 define PAR_GRAp 19 define PAR_RBCt 20 define PAR_WBCt 21 define PAR_WBCt2 22 Use the above identifiers to interpret entries in the array PARAM and FLAG interpretation should happen accordingly This package starts with the X package identifier contains 2 size descriptor bytes SIZE1 and SIZE2 and the XML file in ASCII format Use this as a descriptor of the ...

Page 88: ...RN HT 22 LF P01 HT 6 6 HT 0 LF P02 HT 4 29 HT 0 LF P03 HT 167 HT 0 LF P21 HT 8 2 HT 0 LF P22 HT 5 3 HT 0 LF ETX 7C EOT Receiver acknowledges DATA transmission and requests for RBC package ACK RB Abacus sends RBC package SOH CR STX SNO HT 152 LF DATE HT 19980715 LF TIME HT 114500 LF PID HT 2 LF CHN HT 256 LF 9 HT 16 HT 26 HT 1 HT 0 ETX F2 EOT Receiver acknowledges RBC transmission and requests for ...

Page 89: ...PARN HT 22 LF P01 HT 6 6 HT 0 LF P02 HT 4 29 HT 0 LF P03 HT 167 HT 0 LF P21 HT 8 2 HT 0 LF P22 HT 5 3 HT 0 LF ETX 7C EOT Receiver acknowledges DATA transmission and requests for RBC package ACK RB Abacus sends RBC package SOH CR STX SNO HT 152 LF DATE HT 19980715 LF TIME HT 114500 LF SID HT 2 LF PID HT 26 LF CHN HT 256 LF 9 HT 16 HT 26 HT 1 HT 0 ETX F2 EOT Receiver acknowledges RBC transmission an...

Page 90: ...ARN HT 22 LF P01 HT 6 6 HT 0 LF P02 HT 4 29 HT 0 LF P03 HT 167 HT 0 LF P21 HT 8 2 HT 0 LF P22 HT 5 3 HT 0 LF AGE HT 24 LF ETX 7C EOT A parameter called AGE is included in the header info This represents the age of the given patient if selected in service menu and defined at sample info screen The interpretation of this value is If the age value is greater than 128 then it represents months in the ...

Page 91: ...th 4 1 12 16 2 4 4 bits unsigned year 12 0 4095 4096 2 12 12 bits DateTime define MaxParams 23 struct TMeasData WORD RecNo 2 bytes DateTime TimeStamp 4 bytes WORD OperatorID 2 bytes char SampleID 8 8 bytes char PatientID 20 20 bytes char Name 32 32 bytes DateTime BirthDate 4 bytes BYTE Sex 1 byte char Doctor 16 16 bytes float LimitsLow MaxParams 23 4 92 bytes float LimitsHigh MaxParams 23 4 92 byt...

Page 92: ...6 values 7 256 BYTE PLTlo Lower threshold of PLT graph 7 1 BYTE PLThi Upper threshold of PLT graph 7 1 BYTE RBClo Lower threshold of RBC graph 7 1 BYTE WBClo Lower threshold of WBC graph 7 1 BYTE LYMhi Upper threshold of LYMs on WBC graph 7 1 BYTE GRAlo Lower threshold of GRAs on WBC graph 7 1 BYTE Param Array of measured values 23 elements 5 23 x 4 bytes float array Flag Array of flags of measure...

Page 93: ...er arrays space not used within a string is always filled with 0 s String to store George Gets stored on 20 chracters as G e o r g e 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Note 4 Sex is decoded as Sex 0 Not specified Sex 1 Male Sex 2 Female Note 5 Float numbers are stored on 4 bytes according to the IEEE 745 standard Note 6 Vetmode stores the internal database identifier for the record It is of no importance...

Page 94: ...ts Each value is stored on 1 byte Actual Probe Voltage of given measurement can be calculated as PrVxxxx 50V 1024 Note 12 Age field interpretation Age_unit Age 128 MONTH YEAR if Age_unit MONTH Age_of_patient Age 128 this in MONTHS else Age_of_patient Age this in YEARS Note depending on database settings either BirthDate or Age gets filled LimitsLow LimitsHigh and Param match in their structure the...

Page 95: ...Abacus Service Manual rev 1 09 95 10 3 Abacus cabling diagram ...

Page 96: ...hematics Left open Abacus Csövezés Version 1 4 2007 03 14 A Tubing vsd W C L D L D D D P PUFF 1 2 4 8 M 1 V0 2 1 V1 2 2 3 1 V3 1 V2 2 2 3 1 V4 2 3 1 V5 1 2 2 3 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3 1 Pump RBC WBC MIX V12 V11 V10 V9 V8 V7 V6 Tubing schematics ...

Page 97: ...s Electronic schematics can be found in the following Appendices in PDF Board name File name COMB board AJ Comb_v4 12 PPB board AJ PPB_v4 0 IDEEPROM board AJ IDBoard_v3 0 Measuring board AMB_v1 2 Dilutor Opto board AJ DilOpt2M_v3 1 Micro Dilutor board AJ MicroD_v3 0 Display board AJ DispIC_v4 0 Keypad AP keypad_v1 2 Reagent Sensor board AJ ReagS_v3 0 START button AP Start_v1 0 XYD Opto board AE XY...

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