6
3.
Quick Guides
Basic Procedure
1.
With the instrument arm in the down position, launch the app of interest.
o
Optional: Enter a sample name and additional sample information.
2.
Select or add a new protocol.
o
Optional: Optimize image and count settings.
3.
With the arm down, dispense the sample onto the measurement surface.
o
Note: A slide may be used when counting biohazard samples. Choose “Slide” mode in
protocol.
4.
Focus the image so that brightfield cells are visible with a bright center and a dark outline (figure
1).
5.
Ensure the cells are no longer moving and then tap the Count button.
6.
Once measurement is complete, wipe away sample with a clean dry laboratory wipe and close the
arm
Best Practices
•
Ensure surface is clean prior to dispensing a sample onto the measurement surface.
•
Vortex samples before loading for best results.
•
Ensure all sample concentrations fall within concentration limits of the instrument for accurate
results.
o
Use the appropriate protocol settings for Chamber Height when measuring high or low
concentration samples, or when using slides.
o
Note: Refer to the text located in the Count button background field for the proper loading
volume when measuring low or high-density samples.
•
Lower the arm before dispensing the sample onto the surface.
•
Ensure cells have settled to the lower surface before tapping the Count button. The time for cells
to settle may vary by cell type and should be determined empirically.
•
Avoid introducing air bubbles into the sample solution when mixing or dispensing samples.
•
Refer to DeNovix assay protocols or other manufacturer guidelines for cell dyes such as Acridine
Orange, Propidium Iodide or Fluorescein Diacetate for assay specific instructions.
•
Optimize Focus and Exposure settings to ensure the algorithms deliver the most accurate results.
Focus Example: 1) Properly focused 2) Improper focus
Exposure Example: A) Correct B) Exposure too high C) Exposure too low
Summary of Contents for CellDrop
Page 1: ...rev MAR 27 2019 ...
