5
3.5 Overloading rotors
The maximum load of the rotor and the maximum speed have been established by the
manufacturer. Do not attempt to exceed these values. The maximum speed of the rotor
has been established for liquids having a homogeneous density of 1.2 g/ml or less. It is
necessary to reduce the speed in order to centrifuge liquids with a higher density.
Failure to reduce the speed may result in damage to the rotor and centrifuge.
The
revised maximum speed can be calculated with the following formula
:
Reduced speed (n
red
) =
x max speed (n
max
)
Example:
Where the density of the liquid is 1.7, the new maximum speed would be calculated as
follows:
n
red
=
x 13,300 = 11,174 rpm
If in doubt concerning maximum speeds, please contact the manufacturer for
assistance.
4. Operation
ATTENTION: Never attempt to operate the centrifuge with rotors or adapters
that show signs of corrosion or mechanical damage. Never centrifuge
strongly corrosive materials that may damage the rotors, accessories, or bowl
of the unit.
4.1 Attaching rotor lid
After the rotor has been properly secured and loaded, attach the rotor lid to
the rotor. Always use the rotor lid for safety and to allow the rotor to reach
proper speed. Make sure that the rotor lid snaps securely into place, by
pressing down on center catch.
4.2 Closing the lid
Close the centrifuge lid. The Corning
LSE
Microcentrifuge has a lid lock that
activates only when a run is started.
4.3 Lid release
The lid will remain locked during a centrifuge run. Once the run has been
completed and the rotor has come to a stop, a beep will indicate the end
of a run, and the lid will unlock automatically.
