Consort E3100, Manual

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E3xxx E3xxx
99
Solutions Solutions
1x TAE 40 mM tris (pH 7.6), 20 mM acetic acid, 1 mM EDTA
• 50 x (1l) dissolve in 750 ml distilled water:
• 242 g tris base (FW = 121)
• 57.1 ml glacial acetic acid
• 100 ml 0.5 M EDTA (pH 8.0).
• Fill to 1 litre with distilled water.
1x TBE 89 mM tris (pH 7.6), 89 mM boric acid, 2 mM EDTA
• 10 x (1l) dissolve in 750 ml distilled water:
• 108 g tris base (FW = 121)
• 55 g boric acid (FW = 61.8)
• 40 ml 0.5 M EDTA (pH 8.0)
• Fill to 1 litre with distilled water.
Sample Loading Dye
• 10x sample buffer stock consists of 50% glycerol, 0.25%
bromophenol blue, and 0.25% xylene cyanole FF in 1x TAE
buffer. Only 1–10 ml of the 10x loading dye should be pre-
pared.
Ethidium Bromide Solution
• Add 10 mg of Ethidium Bromide to 1 ml distilled water.