Consort E3100, Manual

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E3xxx E3xxx
55
Gel preparation Gel preparation
1
. Table below shows the volume of agarose solution required to
make the desired agarose gel (5 mm thick) for each unit tray
size. For a standard 0.7 % agarose gel, add 0.7 g of agarose to
100 ml of 1x TAE or TBE solution. The same 1x solution should
be used in the tank buffer solution.
Model Gel size (cm) Volume (ml)
E3100 10x8 40
E3200 7x7 25
7x10 35
E3300 10x7 35
10x10 50
E3400 15x7 53
15x10 75
15x15 113
E3500
20x10 100
20x20 200
E3600
26x16 208
26x24 312
26x32 416
2
. Add the agarose powder to a conical flask.
3 . Add the appropriate amount of 1 x TAE or TBE solution from
the table above. To prevent evaporation during the dissolving
steps below, the conical flask should be covered with para-
film.
4
. Dissolve the agarose powder by heating the agarose either on
a magnetic hot plate with stirring bar or in a microwave oven.
If using the microwave method, the microwave should be set
at around a 400 W or medium setting and the flask swirled
every minute. The solution should be heated until all crystals
are dissolved. This is best viewed against a light background.
Crystals appear as translucent crystals. These will interfere
with sample migration if not completely dissolved.
5
.
The gel must be cooled to between 50°C and 60°C degrees
before pouring.