Use of the S100x oil-immersion objective
Some Cole-Parmer MSU-200 Series microscopes are equipped with an S100x N.A. 1.25 oil immersion objective. Please
follow the instructions below on how to use this objective:
1.
Remove the dust protection cap from the revolving nosepiece to mount the S100x objective
2.
Focus the image with the S40x objective
3.
Lock the slide protection handle
4.
Turn the revolving nosepiece so that the S100x objective almost reaches the click-stop
5.
Put a small drop of immersion oil on the center of the slide
6.
Now turn the S100x objective so that you feel the click stop
7.
The front lens is in contact with the immersion oil
8.
Look through the eyepiece and focus on the image with the fine adjustment knobs
9.
The distance between the lens of the objective and the slide is very small!
10.
In case there are small bubbles visible, turn the S100x objective a couple of times from left to right so that the front
of the objective moves in the oil and the bubbles will disappear
11.
After using the S100x objective, loosen the slide protection handle and turn the table with the course
adjustment knobs downwards until the front lens no longer touches the oil. Clean the front lens of the S100x
objective
12.
Always clean the front lens of the S100x objective with a piece of lens paper that is moistened with a drop of
isopropanol.
13.
Clean the slide after use as well
Caution
•
Never put a drop of xylol or alcohol directly on the lens of the objective. It may enter the objective and
dissolve the glue that holds the lenses!
•
Avoid oil contact with any of the other objectives!
Safety device
All types are equipped with a pre-fixed safety device to prevent damage to the objective lens or the slide
It is recommended to use slides with a thickness of 1.0 – 1.2 mm in combination with cover glasses with a thickness of
0.13 mm or 0.17 mm
Illumination
The LED illumination of the MSU-200 Series is equipped with rechargeable batteries. The length of use after charging
is about 60 hours. The full charging time is about 10 hours. Before first use the batteries have to be fully charged.
Connect the cable to the mains socket. The illumination has the following specifications:
•
LED
: 1 W, 300 mA
•
Charger
: Primary 100-240 V, 50/60 Hz, 5 W
•
Batteries
: 3 NiMh, AA type, 1.2 Volt 1600 - 2600 mA
Maintenance and cleaning
Always cover your MSU-200 Series microscope with a dust cover after use. Always keep the eyepiece(s) and objectives
mounted on the microscope to avoid dust entering the instrument
Cleaning the optics
When the eyepiece lens or front lens of the 10x or S40x objective are dirty, they can be cleaned by wiping a piece of
lens paper over the surface (circular movements). When this does not help put a drop of alcohol on the lens paper and
wipe it.
Never put xylol or alcohol directly on the lens!
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