Placing the specimen slide
Slides go into the clamp of the mechanical stage (N) where it can be moved with precision into X- and Y- directions
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Push the arm of the specimen holder backwards
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Release the arm slowly clamping the slide with the cover glass facing up
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Rotating the X and Y-axis knob will move the specimen to the center for alignment with the center of the
objective
Focusing and slide protection
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Select the 4x objective and make sure that it is placed correctly in the optical path
•
Move the stage to the top by using the coarse adjustment knob and focus till the image appears
•
Rotate the fine focusing knob to bring the image into focus
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When you focus with a S100x objective, you need to lock the slide protection handle. The slide protection
handle protects the slide by limiting the travel range of the mechanical stage. This way the objective will not
touch or break your slides
Adjusting the focusing tension
The tension of the focusing knobs can be adjusted. You can set it from light to heavy according to your own
preference. Please note that when the specimen leaves the focus plane after focusing or the stage declines out of its
own, then you need to adjust the tension. To tighten the focusing knob (more heavy), rotate the tension adjustment
ring counter-clockwise; to loosen it, turn it in the clockwise direction.
Eyepieces
Using a binocular (or trinocular) e, we recommend you to go through the steps below:
1. The interpupillary distance
The correct interpupillary distance is reached when one round image is seen in the field of view (see image below).
This distance can be set by either pushing the tubes towards each other or pulling them away from each other. This
distance is different for each observer and thus should be set individually. When more users are working with the
microscope it is recommended to remember your interpupillary distance for a quick set-up during new microscopy
sessions
2. The correct eye point
The eye point is the distance from the eyepiece to the user’s pupil. To obtain the correct eye point, move the eyes
towards the eyepieces until a sharp image is reached at a full field of view
Field of view
before adjustment
Field of view
after adjustment
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