12
Use of the S100x oil-immersion objective
Some Cole-Parmer MSU-100 Series microscopes are equipped with an S100x N.A. 1.25 oil immersion objective. Please
follow the instructions below on how to use this objective:
1.
Remove the dust protection cap from the revolving nosepiece to mount the S100x objective (the objective can
be premounted)
2.
Focus the image with the S40x objective
3.
Turn the revolving nosepiece so the S100x objective almost reaches the click-stop
4.
Put a small drop of immersion oil on the center of the slide
5.
Now turn the S100x objective so that you feel the click stop
6.
The front lens is in contact with the immersion oil
7.
Look through the eyepiece and focus the image with the fine adjustment knobs
8.
The distance between the lens of the objective and the slide is very small!
9.
In case there are small bubbles visible, turn the S100x objective a couple of times from left to right so that the
front of the objective moves in the oil and the bubbles will disappear
10.
After using the S100x objective, loosen the slide protection handle and turn the table with the course
adjustment knobs downwards until the front lens doesn’t touch the oil any longer. Clean the front lens of the
S100x objective
11.
Always clean the front lens of the S100x objective with a piece of lens paper that is moistened with a drop of
isopropanol.
12.
Clean the slide after use as well
Caution
•
Never put a drop of xylol or alcohol directly on the lens of the objective. It could enter the objective and dissolve
the glue that holds the lenses!
•
Avoid oil contact with any of the other objectives!
Safety device
To prevent damage to the objective lens, or breaking the slide, all types are equipped with a pre-fixed safety device.
It is recommended to use slides of 1.0 – 1.2 mm thickness in combination with cover glasses of 0.13 mm or 0.17 mm
thickness
Illumination
The LED illumination of the MSU-100 Series is equipped with rechargeable batteries. The length of use after charging is
about 48 hours. The full charging time is about 10 hours. At first use the batteries will have to be fully charged. Connect
the external power supply unit to the power inlet socket. For all models with batteries it is highly recommended to use
the microscope with power supply disconnected to keep the batteries in good working condition and recharge after
batteries run empty
The illumination has the following specifications:
•
1W NeoLED
: 1W, 300mA equipped with Fresnel lens
•
External Charger : Primary 100-240 VAC, 50/60 Hz, 0.3 A
•
Batteries
: 3 NiMh, AA type, 1.2 Volt 1600 - 2000 mA.
