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Cleaver Scientific gelLITE, Manual

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Cleaver Scientific gelLITE Manual Download Page 42

 

42 

 

Chapter 

Six- 

Troubleshooting 

and 

General 

Information 
 
 

If 

your 

dye/reagent 

is 

not 

listed 

then 

please 

contact 

[email protected]

  

 

6.1 

Technical Information 

 
genePIX software hardware installation guide  
 

Please  visit  the  Cleaver  Scientific  website  to  download  the  gelLITE  technical 
literature (This is also available on your genePIX USB memory stick). 

 

Quick Guides 

 

gelLITE Installation 
gelLITE Image Capture

 

 
6.2 

Technical Assistance 

 

Cleaver Scientific Ltd. 
Unit 41,Somers Road Industrial Estate  
Rugby 
CV22 7DH 
Tel: +44 (0)1788 565300 
Fax: +44 (0)1788 552822 
Email: 

[email protected]

 

 

 
 
 
 
 
 
 
 

Summary of Contents for gelLITE

Page 1: ...1 gelLITE manual...

Page 2: ...zard 1 3 Enabling secure user accounts 1 4 Setting up hardware Chapter Two Auto Capture and Manual Image Acquisition 2 0 Auto Capture 2 0 1 Sample Selection 2 0 2 Dye Selection 2 0 3 Protocol Selectio...

Page 3: ...figurations 5 1 Saving Configurations 5 1 1 Saving Configurations in Autocapture mode 5 1 2 Saving Configurations in Manual mode 5 1 3 Opening Configurations Chapter Six Troubleshooting and General In...

Page 4: ...ont door and an internal white light 1 1 Applications supported Transilluminator UV White Light Pad Blue LED Transilluminator Ethidium Bromide RunSAFE SYBR Green SYBR Gold SYBR Safe Gel Green Gel Red...

Page 5: ...ty CAMERA CMOS Digital camera USB2 connection FILTER DRAWER Use a range of filters for extensive choice of fluorescent applications SAFETY SWITCH Protects from accidental UV exposure when opening the...

Page 6: ...afe blue as well as protein gels stained with colorimetric stains such as Coomassie and Silver stains 1 2 3 2 proBLUEVIEW transilluminator The proBLUEVIEW transilluminator is ideal for safely imaging...

Page 7: ...sions of Windows operating systems are not supported 1 2 4 3 Software Installation This section provides information regarding the installation of genePIX image capture software You will need to insta...

Page 8: ...ext Select from the list the hardware that you have then click Next Specify CFR settings you would like to enable If you are not working within a CFR compliant environment leave all boxes unchecked Cl...

Page 9: ...more folder options Click Next 1 2 4 6 Select Start Menu Folder Please select where you would like to create the shortcuts for the program To select a different folder click the browse button Click Ne...

Page 10: ...ick Next Click Finish to complete the Device Driver Installation Wizard 1 3 Enabling secure user accounts To enable a secure user account you must use your Microsoft Windows account log in Secure user...

Page 11: ...preferences on the type of report settings that you would like You can select to view or print the full report Image File description and capture properties or the basic report image and capture setti...

Page 12: ...12 Figure 2 User Preferences page...

Page 13: ...that form a structured workflow The workflow is very easy to navigate through by using the green bouncing arrow present in the bottom right hand corner of the screen to move forward to the next screen...

Page 14: ...ng conditions for your sample based on the excitation and emission curves of the majority of dyes currently commercially available 2 0 4 Sample Positioning The sample positioning screen shows a live i...

Page 15: ...either from your recent selections only or by searching for a dye N B untick recent selections only to search the full list Step Four Check the Select Light and Filter box to see the best imaging con...

Page 16: ...bright image If you wish to perform EDR please check the EDR box Step Six The captured image will appear in the main window The image can now be saved in various file formats sgd TIFF JPG and BMP or p...

Page 17: ...y or by searching for a dye N B untick recent selections only to search the full list Step Four Check the Select Light and Filter box to see the best imaging condition for your hardware set up Step Fi...

Page 18: ...ort the image to geneQUANT analysis software To ensure that the image you are capturing is not saturated select the Show Saturation button Figure 5 Saturation will only show when the slider bars have...

Page 19: ...e on screen instructions Figure 8 Figure 7 Dynamic Fielding check box Depending on which type of sample UV epi lights or transilluminator white light you would like to Dynamic field you may get either...

Page 20: ...ting and filters present in your system Figure 10 Figure 10 Filter Light selection Once you have selected a light and filter you need to set the exposure time Use the buttons to increase or decrease t...

Page 21: ...ed red show that these bands are saturated genePIX software also has the ability to Dynamic field The dynamic field function is based on powerful algorithms which corrects for uneven illumination This...

Page 22: ...Stop buttons The histogram tool is very useful for illustrating the distribution of grey scales within the image Figure 18 The level of grey scales produced is dependent on exposure time up to 65536...

Page 23: ...rmation button also provides information on the range of black and white levels You can also point the mouse arrow over a band and the black and white levels and XY coordinates appear in this box Figu...

Page 24: ...s will remove the image from every image pool If the image has not been saved the software will prompt you to save the image before removing it Figure 24 The Close button The image pool also permits t...

Page 25: ...you can export an image save and print images 3 1 Saving Images Images captured with genePIX software can be saved in several formats To save an image simply press touch screen or click on the followi...

Page 26: ...e displayed image select between bmp and jpg formats N B BMP and jpg formats will save as 8 bit files and you will therefore lose quantitative data This format should only be used for the export of vi...

Page 27: ...rting Images to geneQUANT analysis software Various file formats can be exported from genePIX to geneQUANT Cleaver Scientific recommends uncompressed TIFF or sgd to maintain quantification To export a...

Page 28: ...the top left hand side of the screen allowing you to switch between annotation enhancement tools and 3D view Figure 33 Figure 33 screen annotation enhancement and 3D icons The icons from left to right...

Page 29: ...the text double click on it A window will appear enabling selection of font size and style The text box can be dragged around the screen as can all the annotation This tool can be used when you wish t...

Page 30: ...annotated objects i e the line thickness and style can be altered using the drop down menu Any annotations made will be automatically saved when images are in SGD format When saving in other formats y...

Page 31: ...rsor can then be used to freely rotate the image b To finalise fix the rotation click button a To reset the orientation of the image press c Figure 39 Rotation icons a rotation fix button b arrow curs...

Page 32: ...harpen flip and crop the image Figure 42 Figure 42 Image Enhancement tools Click on this icon to apply a sharpening filter to your image When this filter has been applied band edges should become more...

Page 33: ...aced in the cabinet the wrong way round Select icon allows you to select an area of interest within the captured image The crop icon will crop the selected area of interest and paste into a separate w...

Page 34: ...with a mouse to position as required Use the Gamma slider to adjust the gamma of the image to see fainter bands Figure 45 Figure 45 3D View gamma slider Adding a false colour wash can improve the con...

Page 35: ...ns To remove any of the images from this pool simply select the Remove button If you wish to remove all the images from the image pool select the Remove All button Figure 47 Figure 47 Remove image but...

Page 36: ...e Browse Images screen Figure 50 Figure 50 View Images Screen The zoom slider can be used to increase or decrease the size of the image Figure 51 Figure 51 View Screen digital zoom slider bar The View...

Page 37: ...Zoom and Range slider bars To adjust the zoom setting of the image s use the slider on the left hand side Move the slider bar towards the to zoom in and towards the to zoom out To adjust the brightne...

Page 38: ...from the change layout heading 4 9 Load Images screen The Load images screen allows you to open files that are already saved on your computer and to open files from external devices such as USB keys...

Page 39: ...39 Figure 57 Browse screen...

Page 40: ...Sample positioning Once you have captured an image you can save this as a configuration by selecting the following icon Figure 58 Figure 58 Configuration Icon A pop up window will appear where you en...

Page 41: ...ck or Make public the configuration Figure 59 5 1 3 Opening Configurations From the home page you can open saved configurations by simply pressing or selecting the configuration you wish to open Once...

Page 42: ...allation guide Please visit the Cleaver Scientific website to download the gelLITE technical literature This is also available on your genePIX USB memory stick Quick Guides gelLITE Installation gelLIT...

Page 43: ...s that you may have on the hardware screen Dye not in list Your dye is not in the database Contact support Cleaverscientific com Dye name is greyed out You do not have the appropriate hardware to imag...

Page 44: ...two types of image sources original and processed If you have made enhancements to your image such as sharpening smoothing and cropping an area this will alter the image source and the image will now...

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