21
To ensure that the image you are capturing is not saturated select the ‘Show
Saturation button’ (
Figure 13
). Saturation will only show when the slider bars
have been moved to each end of the brightness/contrast slider. Over-exposed
(saturated) white bands will show red and over-exposed black areas in blue
(
Figure 14
).
Figure 13- Show Saturation button
Figure 14- Image of a gel showing saturation
The bands coloured red show that these bands are saturated
genePIX software also has the ability to Dynamic field. The dynamic field function
is based on powerful algorithms which corrects for uneven illumination. This
results in an image with a flat, even background whilst maintaining GLP
compliance. To Dynamic field check the box for dynamic fielding (
Figure 15
) and
then follow the on-screen instructions (
Figure 16
).
Figure 15- Dynamic Fielding check box
Depending on which type of sample (UV, epi lights or transilluminator white light)
you would like to Dynamic field you may get either of the bottom two messages
shown below.
Summary of Contents for gelLITE
Page 1: ...1 gelLITE manual...
Page 12: ...12 Figure 2 User Preferences page...
Page 39: ...39 Figure 57 Browse screen...
