20
2.3
Manual Capture
Manual capture offers you the ability to take complete control of every function of
the system. The manual mode is particularly useful for the more unusual
applications and if you have known imaging parameters that you wish to use.
Once Manual capture has been selected the camera is live. To be able to
position your sample the appropriate lighting and filter combination need to be
selected. Both the lighting and filter menus have a drop-down menu where you
can select from all the available lighting and filters present in your system
(
Figure 10
).
Figure 10
– Filter/Light selection
Once you have selected a light and filter you need to set the exposure time. Use
the +/- buttons to increase or decrease the exposure time (
Figure 11
). The
exposure time can be entered manually by typing a number directly into the box.
Please
enter
the
exposure
time
in
the
following
format
hours:minutes:seconds:milliseconds (h:m:s:ms). There is a default exposure time
of 80ms for fluorescent gels.
Figure 11 - Setting Exposure times
Auto Capture is designed to capture the maximum available grey scales of the
sample without actually allowing saturation. Therefore, this feature is ideal for
quantifying data (
Figure 12
).
Figure 12- Auto Capture button
Summary of Contents for gelLITE
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Page 12: ...12 Figure 2 User Preferences page...
Page 39: ...39 Figure 57 Browse screen...
