ENG
2016-11-21
Page 13 of 44
CertoClav Sterilizer GmbH
Georg Grinninger-Str. 37
A-4050 Traun / Austria
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Please follow the following instructions:
Close the lid after confirming that no foreign matter is adhering to the section contacting the lid gasket.
Foreign matter in this section can cause vapor leaks. When using a waste processing bag or other
kind of bag and disinfecting, place the bag in the metal mesh holder and then insert it into the
chamber. Using the bag “as is” can cause excessive temperatures, pressures, lack-of-water, etc.
Be careful not to pinch hands when closing the lid. Always use the base plate.
Be sure that the temperature has dropped to at least 50°C in the chamber before the beginning of the
next cycle.
If a waste disposal bag is used in sterilization, open the bag enough that the bag is not in contact with
the inside surface of the chamber. Insufficient sterilization may be caused if the bag is closed during
sterilization. When the bag is opened excessively, steam is prevented from circulating in the chamber.
This may also result in insufficient sterilization.
Do not pile specimen on top of one another. When the chamber is overly packed, steam fails to
penetrate all points, resulting in an incomplete sterilization.
In sterilizing liquids such as chemicals and media pay attention to the quantity of the liquid in relation
to its container. For an Erlenmeyer flask, the amount of chemical should be approx. 3/4 of the capacity
of the container; for a test tube, the appropriate quantity of chemical is approx. half of the capacity of
the container. Too much chemical may result in overflow from the container during the temperature
rising or cooling process.
Use container caps that are loose fitting and allow the passage of air. Containers may break if venting
is not possible.
In the case of dissolution of agar medium, its quantity should be 2 liters or less per container. Two
liters or more of agar medium may not be completely dissolved.
Closing lid
Please check that the lid seal is correctly inserted. The signature "Place This Side Facing Down" must
be readable. See figure below. Then close the lid in exactly the opposite way, as it was previously
opened.
