Discover Bio
™
Manual Microwave Peptide Synthesizer
600423 • Revision 2 • August 2017
5
4. Set up the Waste bottle.
4.1. Connect a bottle cap to the 1 L bottle to be used for waste. Cut the dip tube to
approximately 1.5” (~4 cm) in length.
4.2. Connect the bottle tubing to the cap. The tube labeled “LIQ” should be connected to the
same port as the dip tube. The unlabeled tube should be connected to the port without a dip
tube.
4.3. Connect the other end of the bottle tubing to the rear of the Wash Station. The tube labeled
“LIQ” should be connected to position 6. The unlabeled tube should be connected to position
3.
Nitrogen and Vent Line Setup
1. Connect the nitrogen source to stainless steel port 1. Set the incoming pressure to approximately
20 psi.
2. Cut a piece of tubing (no greater than 10’ (~3 m) in length) and connect it to stainless steel port 2
using a stainless nut. This will serve as the vent line for the entire system. This tubing should be
run into a ventilated fume hood.
Product Collection Position Setup
1. Cut two pieces of tubing approximately 18” (45 cm).
2.
Take one piece of tubing and, using yellow ferrules and nuts, connect one end to the left port on
the rear of the Product Collection Position, then connect the other end to position 10.
3.
Take one piece of tubing and, using yellow ferrules and nuts, connect one end to the right port on
the rear of the Product Collection Position, then connect the other end to position 11.
Standard (25 mL) Vessel Setup
1. Open the reaction vessel by pressing the vessel body up into the vessel top and rotating.
2. Connect the sprayhead line.
2.1. Unscrew the sprayhead. Cut a length of tubing (~48” (120 cm)). Thread one end through the
center port on the reaction vessel top.
2.2. Put a yellow ferrule onto the end of the tubing so that the smaller end is near the end of the
tube, then press the end of the tubing into the sprayhead. Screw the sprayhead back into
the sprayhead port to secure the line.
2.3.
Using a yellow ferrule and nut, connect the other end of the tubing to position 7 on the rear
of the Wash Station.