Discover Bio
™
Manual Microwave Peptide Synthesizer
600423 • Revision 2 • August 2017
14
7. Slide the drain line out of the groove in the top of the vessel assembly. Open the reaction vessel by
pressing the vessel body up and rotating it counterclockwise.
8. Add the cleavage cocktail to the vessel. Seal the vessel by pressing the vessel body up into the
vessel top and rotating. Slide the drain line back into the groove in the top of the vessel assembly.
Place the vessel in the Discover.
9.
Load the Cleavage microwave method, and press the
Play/Pause key to begin the method.
10.
When the microwave is finished, remove the reaction vessel from the Discover cavity and place it in
the vessel holder.
11.
Connect a clean, labeled centrifuge tube to the product manifold.
12. Turn the right knob to “Product” to drain the cleaved peptide into the centrifuge tube.
13. Remove the centrifuge tube containing the cleaved peptide. The peptide is now ready to be
precipitated and analyzed.
14. Set the right knob to “Stop”. Set the left knob to “Wash Solvent” and add approximately 7 mL of
DCM to the vessel. Set the left know to “N2 Purge (Wash)”, then set the right knob to “Waste” to
drain the reaction vessel. Set both knobs to “Stop”.
15. Repeat step 12 two more times.
16. Open the reaction vessel and remove the spent resin for disposal.
17. Repeat step 12.
18. Set both knobs to “Stop.” Turn off the nitrogen source.
19.
Remove the solvent bottle, then connect a solvent bottle of wash solvent.
20. Turn on the nitrogen source.