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3.5 Preparation of the Cassettes
1. After polymerization rinse gel plate assembly with D.I. water to remove excess
acrylamide or denaturants from plate exterior.
2. Remove comb and quickly transfer gel sandwich to cassette by removing all #2
clamps.
LEAVE GEL WRAP IN PLACE
. The Gel Wrap acts as a barrier and prevents
perpendicular electrical fields from intenferring with outside lanes. Re-clamp to
cassette using #1 clamps/4 per side. Dislodge by pulling the bottom section of the Gel
Wrap from the plates to allow buffer contact with the gel. See figure 3.8.
3. Immerse cassette into pre-heated buffer tank (fig.3.8), and attach recirculating tubing to
each upper reservoir (fig. 3.9 a,b,c). Rinse loading interface of gel with tank buffer to
remove non-polymerized acrylamide or excess urea. Rinse each well with buffer and fill
upper reservoir
Dislodge Gelwrap
Fig. 3-8
