C.B.S. SCIENTIFIC DGGE-1001, Instruction Manual

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SECTION 1
General Information
1.1 Introduction
Denaturing Gradient Gel Electrophoresis (DGGE) is a powerful genetic analysis technique that can be used
for detecting single base changes and polymorphisms in genomic (1, 2), cloned, and PCR amplified DNA
(3,4). Two of the most valuable uses for DGGE in human genetics are in directly detecting single base
changes that cause disease and in detecting polymorphisms with DNA probes for genetic-linkage analysis.
Also, DNA fragment melting points can be determined using Perpendicular DGGE (1).
C.B.S. Scientific has designed five different DGGE Systems, which are reliable and easy-to-use. The
DGGE-1001 is a 2gel system and features one dual gel cassette. The DGGE-2001 features two single gel
cassettes. The DGGE-2401 has the same buffer tank as the DGGE-1001 and DGGE-2001 but features two
dual gel cassettes, for a capacity of 4 gels per run. Also available is the DGGE-4001, which has a larger
buffer tank and four single cassettes. Finally there is the DGGE-4801, which features the larger buffer tank
and four dual cassettes enabling the researcher to run up to 8 gels simultaneously. Improvements to the
technique include: a simplified method for casting perpendicular and vertical gels using Gel Wrap
®
, single or
dual gel cassettes have been redesigned eliminating the use of agarose plugs, the need for an external
peristaltic pump for buffer cycling has been eliminated, bulky screw clamps have been replaced with
polypropylene spring clamps, buffer tank dimensions have been changed to use bench space more
efficiently, and a safety cover with an electrical interlock has been added for protection and to help maintain
temperature and reduce evaporation.
1.2 Specifications
Constructions
DGGE Tank A
crylic
DGGE Lid
Sodalime glass, acrylic
Electrodes
Platinum wire .010" diameter
Power cords
FR Silicone, rated 7500V,
200mA, 65
°
C
DGGE Cassette
Acrylic
Heater/Stirrer Buffer Cycler
Stainless steel
Combs
Teflon
Spacers
Polymer
Glass Plates
Sodalime or Borosilicate
Gradient Maker
Acrylic, teflon, stainless steel
DGGE-
1001
DGGE-
2001
DGGE-
2401
DGGE-
4001
DGGE-
4801
Shipping weight 40lbs 58lbs 58lbs 68lbs 108lbs
Gel Size
15x20cm 15x20cm 15x20cm 15x20cm 15x20cm
Buffer Volume 15.2 L** 21 L 21L
Distance between
electrodes
20cm 20cm 20cm 20cm 20cm
Voltage limit 250 250 250 250 250
**
requires the use of a buffer displacement chamber