TABLE OF CONTENTS
Page
Important
User
Information
3-4
Section 1
General Information
1.1
Introduction
5
1.2
Specifications
5
1.3
Safety
6
Section
2
Description
of
parts
2.1
Unpacking
7
2.2
Components/Assembly
7
Section
3
Instructions
for
Use
3.1
Unit
Set-up
and
Unpacking
8-11
3.2
Preparation/Cleaning of Glass Plates for Gel Casting
12
3.3
Gel
Casting
Techniques
12-16
3.4
Vertical
Gradient
Formation 17
3.5
Preparation
of
the
Cassettes
18
3.6
Buffer
Cycling
Connections 19-20
3.7
Running
the
Gels
21
3.8
Removing
the
Gels
22
3.9
Perpeniduclar
Gel
Casting
23
3.10
Perpendicular
Gradient
Formation 24-25
3.11 Buffer Siphon Pump Instructions for use
26
3.12
Maximum
Well/Comb
Volumes
27
Section
4
Running
Conditions
4.1
Recommended
Power
27
4.2
Recommended
Buffers
27-28
4.3
References
28
Section 5
Maintenance of Equipment
5.1
Care
and
Handling
29
5.2
Maintenance
29
Section 6
DGGE System and Accessories
30-31