Gemini Series Electroporator User’s Manual
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Table of Contents
Safety Information .......................................................................................................................2
Introduction .................................................................................................................................3
Table of Contents ........................................................................................................................4
Product Overview ........................................................................................................................5
Electroporation Overview ............................................................................................................6
General Optimization Guide for Electroporation .................................................................... 7-8
Wave Forms ..................................................................................................................................... 7
Field Strength ................................................................................................................................... 7
Pulse Length ..................................................................................................................................... 8
Number of Pulses ............................................................................................................................. 8
Electroporation Buffer ...................................................................................................................... 8
DNA/RNA Concentrations ................................................................................................................. 8
Applications ........................................................................................................................... 9-11
Mammalian Cell Transfection ............................................................................................................ 9
In Vivo, In Utero, In Ovo ................................................................................................................... 9
Bacteria and Yeast Transformation .................................................................................................... 9
Plant and Insect Transfection ............................................................................................................ 9
References ........................................................................................................................................ 9
General References ...................................................................................................................... 9-11
Glossary of Terms ......................................................................................................................12
Electroporation Buffers .............................................................................................................13
Unpacking the System ...............................................................................................................14
Touchscreen Button Reference ..................................................................................................15
Software Setup .................................................................................................................... 16-17
Setting Time/Date ........................................................................................................................... 16
Setting Audible Alarm Preferences .................................................................................................. 16
Setting Backlight Preferences .......................................................................................................... 17
Displaying Device Information ........................................................................................................ 17
Preset Protocols ................................................................................................................... 18-20
Using Preset Protocols .................................................................................................................... 18
Customizing a Preset Protocol ................................................................................................... 19-20
Performing Experiments ...................................................................................................... 20-26
Creating New Protocols .................................................................................................................. 21
Creating an Exponential Decay Wave Protocol ..................................................................... 21-23
Creating a Square Wave Protocol ........................................................................................ 24-26
Using Specialty Protocols .................................................................................................... 27-29
Using an HT Plate Handler ......................................................................................................... 27-28
Using Specialty Electrodes ......................................................................................................... 28-29
Managing Protocols ............................................................................................................. 30-32
Saving a Copy of a Protocol ............................................................................................................ 30
Renaming a Protocol ...................................................................................................................... 30
Deleting a Protocol ......................................................................................................................... 31
Password Protecting a Protocol ....................................................................................................... 32
Protocol Manager Software ................................................................................................ 33-35
Installation ..................................................................................................................................... 33
Overview ........................................................................................................................................ 33
Upload– Generator to PC ................................................................................................................ 33
Download– PC to Generator ........................................................................................................... 34
Upload Log Files ............................................................................................................................. 35
Remote Control Software ..........................................................................................................36
Generator Specifications ..................................................................................................... 37-38
Maintenance ..............................................................................................................................39
Upgrading Gemini Series Software ..................................................................................... 40-42
SB Virtual Commport Driver Installation ..................................................................................... 40-41
Device Updater Program ................................................................................................................. 42
Error Messages & Troubleshooting ..................................................................................... 43-45
Ordering Information .......................................................................................................... 46-47
Warranty Information ................................................................................................................48
Declaration of Conformity .........................................................................................................49